Declining semen quality and steadying seminal plasma ions in heat‐stressed boar model

Li, Yansen; Wang, Aihua; Taya, Kazuyoshi; Li, Chunmei

doi:10.1007/s12522-015-0205-9

Cited by 15 publications

(12 citation statements)

References 28 publications

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“…Long and Guthrie (2006) used the sperm-rich fraction of a native ejaculate, while we used a full ejaculate that was diluted with a commercial semen extender (Beltsville Thawing Solution; BTS). High concentrations of cations in native ejaculates, especially Zn 2+ and Cu 2+ (Li et al, 2015; Lopez Rodriguez et al, 2013; Strzezek et al, 1995; Lavon and Boursnell, 1975) may have a dose-dependent quenching effect on emitted light in an ATP assay (Wen et al, 2001) and thus could impair the sensitivity of the system. BTS extender contains 3.4 mM EDTA (Johnson et al, 2000) and may have buffered free cation concentrations to low levels.…”

Section: Discussionmentioning

confidence: 99%

Energy metabolic state in hypothermically stored boar spermatozoa using a revised protocol for efficient ATP extraction

et al. 2016

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Mammalian spermatozoa utilize ATP as the energy source for key functions on the route to fertilization. ATP and its precursor nucleotides ADP and AMP are regularly investigated in sperm physiology studies, mostly by bioluminescence assays. Assay results vary widely, mainly due to different efficiencies in nucleotide extraction and prevention of their enzymatic degradation. Here, we describe a revised, validated protocol for efficient phosphatase inhibition and adenine nucleotide extraction resulting in consistently high ATP concentrations exceeding previously reported values for boar spermatozoa up to 20-fold. The revised assay is applicable for determining ATP concentrations and adenylate energy charge in extracts from fresh and frozen samples, thereby allowing simultaneous assessment of semen samples from long-term storage experiments. After validation, the assay was applied to liquid-preserved boar spermatozoa stored at 17°C and 5°C for 24 and 72 h. Cooling to 5°C, but not storage duration, reduced ATP concentration in spermatozoa (P<0.05), which was accompanied by the appearance of AMP and ADP in the preservation medium. ATP and energy charge were highly correlated to the proportion of membrane-intact spermatozoa, supporting the idea of nucleotides leaking through disrupted membranes in cold-shocked cells. The present assay allows highly standardized studies of energy metabolism in spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Energy metabolic state in hypothermically stored boar spermatozoa using a revised protocol for efficient ATP extraction

et al. 2016

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“…This response was likely attributed to the Zninduced testosterone inhibition. Considering that the sperm count would not change un til after 21 d after heat stress exposure (Li et al, 2015), the ESC in this study might not change immediately af ter 8 d of HT. Chronic restraint stress could upregulate the erythropoiesis through glucocorticoid stimulation (Voorhees et al, 2013).…”

Section: Discussionmentioning

confidence: 85%

“…The general homeostatic responses to heat stress in clude reductions in production and increases in water drinking, rectal temperatures, skin temperatures, and breathing rates in animals with a large body size such as cows (Rungruang et al, 2014) and pigs (Pearce et al, 2013). The rectal temperatures increased about 1°C in the Large White boar exposed to the environmental temperature at about 35°C (Li et al, 2015). Another pig breed, Chinese miniature pig, showed an increase about 1.5°C in rectal temperatures during HT at 40°C for 5 h (Yu et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Effects of zinc sulfate pretreatment on heat tolerance of Bama miniature pig under high ambient temperature1

Cao

Zhou

et al. 2015

Journal of Animal Science

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The aim of this study was to evaluate the heat tolerance of Bama miniature pigs under high ambient temperature (40°C) and Zn interactive functions during heat treatment (HT). Bama miniature pigs (male; n = 24; 6-mo old; BW = 10.79 ± 0.06 kg) were randomly allotted to 4 groups and were fed a basal diet or the basal diet supplemented with 1,500 mg of Zn (ZnSO4·H2O)/kg diet for 38 d. At 7 mo of age (d 30), the thermal neutral (TN) groups remained at 25°C, whereas the HT groups were exposed to ambient temperature at 40°C for 5 h daily for 8 consecutive days. Pigs in 4 groups were sacrificed on d 38. Individual rectal temperatures, skin temperatures, and breathing rates were recorded at 3 h after the onset of HT and the blood samples were collected immediately after HT on d 30, 34, and 38. Pigs fed diets with or without Zn doubled their breathing rates (P < 0.05) and increased body surface, scrotal, and rectal temperatures during HT on d 30, 34, and 38, respectively. Zinc supplementation increased BW gain (BWG; P < 0.05) during 38-d experiment period, and HT decreased BWG only from d 30 to 34 (P < 0.05). Heat treatment increased serum testosterone on d 30 (P < 0.05). Zinc supplementation decreased the heat-induced increase of testosterone in HT on d 30 and 34 (P < 0.05). The relative weight of liver increased in HT groups (P < 0.05). Zinc supplementation decreased the relative weights of spleen (P < 0.05) and testis (P < 0.01). The values of abnormal lymphocyte count and large unstained cell count declined approximately 5 times in groups of Zn supplementation, whereas Zn supplementation increased the values of red blood cell count, hemoglobin, hematocrit, mean corpuscular volume, and mean corpuscular hemoglobin. Zinc concentrations increased in serum, liver, kidney, epididymis, longissimus, hair, and feces in groups fed with Zn (P < 0.01). However, additional Zn decreased Zn concentrations in lung, spleen, and testis (P < 0.01). Moreover, HT decreased serum Zn concentrations (P < 0.01). In conclusion, Zn supplementation could be used to alleviate the decline of serum Zn during periods of high ambient temperatures. However, pretreated supplementation with pharmacological Zn did not promote heat tolerance but impacted the erythropoiesis, immunity, and reproductive organ development in Bama miniature pigs.

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“…Cerovský et al, (2005) reported that the effect of heat stress is reflected by the amount of abnormal sperm, with the most frequent abnormality being the occurrence of a protoplasmic drop. Li et al, (2015) measured the temperature in boar in the rectum and scrotum at heat stress when the outside temperature reached above 28 °C for more than 3 days and found that the percentage of morphologically abnormal sperm had increased, but the function of the additional glands was not impaired. Elevated temperature in the scrotum causes impaired spermiogenesis, which leads to higher incidence of abnormally developed sperm (Ahmad et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Elimination the Impact of Heat Stress by Supplementation of Antioxidants Into Diet of Duroc Boars

Přibilová

Horký

Nevrkla

et al. 2018

Acta Univ. Agric. Silvic. Mendelianae Brun.

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The aim of this experiment was to find out, if is it possible to eliminate the impact of heat stress on quality of Duroc boar ejaculate by the addition of complex of antioxidants (Selenium, Zinc, Vit. C and Vit. E) into the basic diet. The experiment was carried out at the station of insemination in Velke Mezirici (Czech Republic) from May to September. For the study were chosen 12 Duroc boars, of approximately the same age and weight, and were divided into two groups. The control group (n = 6) was fed only by basic diet, the experimental group (n = 6) was fed by basic diet with the supplementation of antioxidants in amount: 0.5 mg selenium (seleno-methionine), 70 mg zinc (zinc-methionine), 70 mg vit. E (alpha-tocopherol) and 350 mg vit. C (L-ascorbic acid) per kilogram of basic diet. Monitored parameters of ejaculate was volume of ejaculate (ml), concentration of sperm (10 6 / ml), total rate of sperm (10 9 ), motility of sperm (%) and amount of morphologically abnormal sperm (%). The control group reported slight increase in volume of ejaculate (of 19 %), which is common in summer months. At the same time was the concentration of sperm unstable, during August there was an increase of 15 %, but during September concentration decreased below the initial amount. Motility was around 69 % during whole experiment. There was an increase (of 15 %) in amount of morphologically abnormal sperm, but the result was insignificant. The experimental group had a higher volume of ejaculate, but the diagram of volume of ejaculate was almost the same with the control group. Concentration of sperm increased after the supplementation of antioxidants (of 23 %) and during the experiment decreased only slightly. Motility was about average amount 70 % and was not various during the experiment. In amount of morphologically abnormal sperm there was an increase about 12 %. According to the results, the supplementation of antioxidants had no significant effect on improving of semen quality. But the addition could have a positive effect to stable the parameters of ejaculate.

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Declining semen quality and steadying seminal plasma ions in heat‐stressed boar model

Abstract: A single 3-day heat exposure caused poor semen quality, but did not disturb accessory sex gland secretion in boars. Declining semen quality might be mainly due to the damaged germ cells, which were sensitive to elevated temperature in hot summer months.

Cited by 15 publications

References 28 publications

Energy metabolic state in hypothermically stored boar spermatozoa using a revised protocol for efficient ATP extraction

Energy metabolic state in hypothermically stored boar spermatozoa using a revised protocol for efficient ATP extraction

Effects of zinc sulfate pretreatment on heat tolerance of Bama miniature pig under high ambient temperature1

Elimination the Impact of Heat Stress by Supplementation of Antioxidants Into Diet of Duroc Boars

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