Decoding multifarious role of cow dung bacteria in mobilization of zinc fractions along with growth promotion of C. annuum L.

Abstract

Zinc is one of the micronutrients, required by all types of crops. About 10–100ppm of zinc is present in soil which is generally immobile. The cow dung sustains all life and being practice since aeons. Exploitation of cow dung bacteria can mobilize nutrients besides contributing in sustainable agriculture. Therefore, to examine mobilization of Zn, cow dung is used as a source of bacteria. The objectives of the present study were to isolate an array of bacteria from cow dung and to characterize them for their Zn (ZnO and ZnCO3) mobilization ability in addition to establish the optimum conditions for dissolution of zinc. A total of seventy bacterial isolates have been screened for Zn mobilization. Out of which most potent (CDK15 and CDK25) were selected to study the effect of various parameters viz. pH, temperature and concentration of Zn. These parameters were assessed qualitatively in diverse growth medium and quantitatively using Atomic absorption spectroscopy. Optimum pH and temperature for mobilization was recorded at pH 5 (ZnO) and 37 °C (ZnCO3) by CDK25, whereas, optimum zinc concentration for mobilization was recorded at 0.05% (ZnO) by CDK15. Maximum amount of Zn solubilized was recorded by CDK25 in ZnO (20ppm). Considering the abilities of most potent bacterial isolates with reference to P-mobilization and growth promoting traits, pot culture assay of C. annuum L. was carried out. The findings of which conclude that, bacterium CDK25 (Bacillus megaterium) could be exploited for factors viz. nutrient management of Zn, growth promoting agent, and Zn augmentation in soil.

Bacillus megaterium Strain CDK25, a Novel Plant Growth Promoting Bacterium Enhances Proximate Chemical and Nutritional Composition of Capsicum annuum L

“…of Botany and Microbiology, Gurukula Kangri University, Haridwar (Uttarakhand), India. Both the isolates were obtained from our previous study (Bhatt and Maheshwari, 2019) and maintained in Luria-Bertani medium (LB) comprising the ingredients viz., 5 g yeast extract, 10.0g tryptone, 10.0 g NaCl L −1 respectively, at 30 ± 1°C. The isolates were characterized for various plant growth-promoting assets viz., phosphate solubilization, zinc solubilization, Indole-3-acetic acid production, siderophore production, and hydrogen cyanide production, of which, the phosphate solubilization potential was assessed qualitatively and quantitatively on Pikovskaya's agar medium (Dubey and Maheshwari, 2011).…”

Antibacterial assay guided isolation of a novel hydroxy-substituted pentacyclo ketonic compound from Pseudomonas aeruginosa MBTDCMFRI Ps04

“…Thus, we have employed activity-guided isolation and characterization of the antibacterial secondary metabolite from P. aeruginosa Ps04 with high inhibitory potential against aquaculture pathogens. The productions of secondary metabolites by the organisms are greatly influenced by the physical and chemical environment and their growth conditions [7,[32][33][34][35]. Thus, we have addressed the effects of fermentation medium, incubation time and fermentation conditions such as pH, salinity and temperature to ensure the enhanced production of the antibacterial metabolite from P. aeruginosa Ps04.…”

“…The optimal physiochemical conditions for growth and production of the antibacterial compound by P. aeruginosa Ps04 were assessed by growing the culture in the optimized glycerol alanine medium at various temperatures (25,30,35 and 40°C), pH (6.0, 7.0, 8.0 and 9.0), salinity (5, 10, 15, 20, 25, 30 and 35 ppt), carbon sources (glycerol and glucose at varying concentrations from 0.5-1%) and nitrogen sources (alanine and glutamic acid at varying concentrations from 0.5-1%) for a period of 7 days (at 30°C at 200 rpm) in duplicates. Samples for cell count and antibacterial activity were drawn at every 24 h.…”

Vermicompost and cow dung admixture increases rhizosphere bacterial population and promotes rapid physiological maturity in Maize (Zea mays L.)

“…Their results have showed the increase P availability to plants through phosphate solubilization by PSol. Bhatt and Maheshwari [46] advocated that the cow dung consists of phosphate solubilizing bacteria that help in the early growth of plants. Moreover, the study highlighted the bacteria groups present in the cow dung promote reproductive growth in plants.…”