Decoding the carbon and nitrogen metabolism mechanism in anammox system treating pharmaceutical wastewater with varying COD/N ratios through metagenomic analysis

Chen, Hongying; Li, Xiangkun; Liu, Gaige; Zhu, Jing; Ma, Xiaochen; Piao, Chenyu; You, Shijie; Wang, Ke

doi:10.1016/j.cej.2023.141316

Cited by 17 publications

(9 citation statements)

References 48 publications

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“…Kuenenia stuttgartiensis HNU0043 was mainly responsible for the nitrogen removal of annamox systems. It harbored functional genes of nitrate/nitrite transporter, ammonium transporter, and the formate nitrite transporter (Figure 6), verifying its ability to utilize NH 4 + -N, NO 2 − -N, and NO 3 − -N. 22 The expression level of NIRS in Ca. Kuenenia stuttgartiensis HNU0043 was relatively low in the sidestream anammox processes, indicating that the activity of nitrite reductase was inhibited by cefalexin.…”

Section: Resultsmentioning

confidence: 81%

“…It might be attributed to the promotion of organic matter for the DNRA process of anammox consortia. 22 Whereas, 200 μg L −1 cefalexin reduced their SAAs, which were still higher than those in P0, indicating that the activity of anammox bacteria was not completely inhibited. In addition, .…”

Section: Resultsmentioning

confidence: 89%

“…As shown in Figure , 20 μg L –1 cefalexin caused a significant increase in SAAs of MAR 1 and MAR 2 by 95.7 and 83.4%, respectively. It might be attributed to the promotion of organic matter for the DNRA process of anammox consortia . Whereas, 200 μg L –1 cefalexin reduced their SAAs, which were still higher than those in P0, indicating that the activity of anammox bacteria was not completely inhibited.…”

Section: Resultsmentioning

confidence: 92%

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Mixotrophic Metabolism and Oxygen Detoxification Mitigate Antibiotic Stress in Mainstream Anammox Process Under Microaerobic Condition

Wang,

Han,

Shi

et al. 2024

ACS EST Water

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Given the prevalence and risk of antibiotics and advantages of the anaerobic ammonium oxidation (anammox) process, this study evaluated and compared the mainstream and sidestream anammox process performance in treating wastewater containing typical β-lactam antibiotic under microaerobic conditions. The mainstream anammox process was able to tolerate higher concentrations of cefalexin under the relatively low dissolved oxygen (DO) concentration, while higher DO slightly reduced the nitrogen removal efficiency of the anammox process, but it was still higher than 70%. In contrast, the sidestream anammox process exhibited a poor tolerance to cefalexin stress. Combined with metagenomics and transcriptomics, the comparative analysis of 154 high-quality metagenomic assembly genomes in anammox consortia revealed the potential mechanism of low DO in mitigating cefalexin stress. Results showed that the expressions of genes related to the dissimilatory nitrate reduction to ammonia process and anammox process were significantly upregulated under low DO condition. As the dominant functional bacteria, Candidatus Kuenenia stuttgartiensis HNU0043 had the detoxification mechanism of •O 2 − metabolism and secondary product H 2 O 2 , which mainly depended on the cooperation of Fe−Mn family superoxide dismutase and catalase. Expressions of genes involved in various carbon metabolism pathways were also detected in Ca. Kuenenia stuttgartiensis HNU0043, explaining the resistance of anammox consortia to antibiotic stress through biodegradation. Therefore, the mainstream anammox process tolerated the antibiotic stress under microaerobic conditions, while low DO was more beneficial for maintaining its stability. This work provides insights into the molecular mechanisms underlying anammox microbiota adaptability to antibiotics and oxygen, further expending the implementation of the anammox process.

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Section: Resultsmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 92%

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Mixotrophic Metabolism and Oxygen Detoxification Mitigate Antibiotic Stress in Mainstream Anammox Process Under Microaerobic Condition

Wang,

Han,

Shi

et al. 2024

ACS EST Water

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“…Thanks to microorganisms’ inherent self-solidification properties, AGS maintains its structural integrity even under dynamic conditions, forming agglomerated organisms without swelling or negatively affecting water quality ( Mota et al, 2014 ). This characteristic enables AGS to efficiently remove high concentrations of toxic organic substances, nitrogen, and phosphorus ( Kishida et al, 2009 ; He et al, 2020 ; Chen H. Y. et al, 2023 ).…”

Section: Novel Bio-denitrification Processmentioning

confidence: 99%

Research progress of novel bio-denitrification technology in deep wastewater treatment

Huang,

Fu,

Zhang

et al. 2023

Front. Microbiol.

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Excessive nitrogen emissions are a major contributor to water pollution, posing a threat not only to the environment but also to human health. Therefore, achieving deep denitrification of wastewater is of significant importance. Traditional biological denitrification methods have some drawbacks, including long processing times, substantial land requirements, high energy consumption, and high investment and operational costs. In contrast, the novel bio-denitrification technology reduces the traditional processing time and lowers operational and maintenance costs while improving denitrification efficiency. This technology falls within the category of environmentally friendly, low-energy deep denitrification methods. This paper introduces several innovative bio-denitrification technologies and their combinations, conducts a comparative analysis of their denitrification efficiency across various wastewater types, and concludes by outlining the future prospects for the development of these novel bio-denitrification technologies.

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“…Biodegradable organics are inclined to promote heterotrophic bacteria overgrowth, outcompeting AnAOB. Refractory organics also affect AnAOB activity ( Chen et al, 2023 ; Wang et al, 2020 ; Zhu et al, 2023 ). Thirdly, the COD/TIN of HSAD reject water is significantly higher than that of conventional AD reject water, which doesn't directly satisfy the low COD/TIN requirement of PN/A.…”

Section: Introductionmentioning

confidence: 99%

A two-stage partial nitritation-denitritation/anammox (PN-DN/A) process to treat high-solid anaerobic digestion (HSAD) reject water: Verification based on pilot-scale and full-scale projects

Zhang,

Gong,

Zhu

et al. 2024

Water Research X

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Decoding the carbon and nitrogen metabolism mechanism in anammox system treating pharmaceutical wastewater with varying COD/N ratios through metagenomic analysis

Cited by 17 publications

References 48 publications

Mixotrophic Metabolism and Oxygen Detoxification Mitigate Antibiotic Stress in Mainstream Anammox Process Under Microaerobic Condition

Mixotrophic Metabolism and Oxygen Detoxification Mitigate Antibiotic Stress in Mainstream Anammox Process Under Microaerobic Condition

Research progress of novel bio-denitrification technology in deep wastewater treatment

A two-stage partial nitritation-denitritation/anammox (PN-DN/A) process to treat high-solid anaerobic digestion (HSAD) reject water: Verification based on pilot-scale and full-scale projects

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