Decoding the interspecies interaction in anammox process with inorganic feeding through metagenomic and metatranscriptomic analysis

Ji, Xiaoming; Chen, Zheng; Wang, Yong-Li; Jin, Ren‐Cun

doi:10.1016/j.jclepro.2020.125691

Cited by 45 publications

(10 citation statements)

References 52 publications

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“…Heterotrophs are assumed to be supported by soluble microbial products actively or passively released by autotrophic PN/A members [32]. In addition, others have suggested heterotrophs asessential in providing growth factors to autotrophs [14,33], a claim not consistent with our ndings. Auxotrophy for AA and vitamin biosynthesis were present in both autotrophs as heterotrophs; the most auxotrophic MAGs were heterotrophs, and AnAOB MAGs were the only MAGs encoding potential for cobalamin biosynthesis (Fig 4).…”

Section: Absence Of Nobcontrasting

confidence: 70%

Efficient Management of the Nitritation-Anammox Microbiome Through Intermittent Aeration: Absence of the NOB Guild and Expansion and Diversity of the NOx Reducing Guild Suggests A Highly Reticulated Nitrogen Cycle

Palomo

Silva

Touceda‐Suárez

et al. 2022

Preprint

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Obtaining efficient autotrophic ammonia removal (aka partial nitritation-anammox, or PNA) requires a balanced microbiome with abundant aerobic and anaerobic ammonia oxidizing prokaryotes and scarce nitrite oxidizing bacteria. Here, we analyzed the microbiome of an efficient PNA process that was obtained by sequential feeding and periodic aeration. The genomes of the dominant community members were inferred from metagenomes obtained over a 6 month period. Three Brocadia spp. genomes and three Nitrosomonas spp. genomes dominated the autotrophic community; no NOB genomes were retrieved. Two of the Brocadia spp. genomes lacked the genomic potential for nitrite reduction. A diverse set of heterotrophic genomes was retrieved – each containing genomic potential for only a fraction of the denitrification pathway. A mutual dependency in amino acid and vitamin synthesis was noted between autotrophic and heterotrophic community members. Our analysis suggests a highly-reticulated nitrogen cycle in the examined PNA microbiome with nitric oxide (NO) exchange between the heterotrophs and the anammox guild.

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Section: Absence Of Nobcontrasting

confidence: 70%

Efficient Management of the Nitritation-Anammox Microbiome Through Intermittent Aeration: Absence of the NOB Guild and Expansion and Diversity of the NOx Reducing Guild Suggests A Highly Reticulated Nitrogen Cycle

Palomo

Silva

Touceda‐Suárez

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Heterotrophs are assumed to be supported by soluble microbial products actively or passively released by autotrophic PNA members [32]. In addition, others have suggested heterotrophs as essential in providing growth factors to autotrophs [14,33], a claim not consistent with our findings. Auxotrophy for AA and vitamin biosynthesis were present in both autotrophs as heterotrophs; the most auxotrophic MAGs were heterotrophs, and AnAOB MAGs were the only MAGs encoding potential for cobalamin biosynthesis (Fig.…”

Section: The Types Of Hb and The Role Of Auxotrophy Vs Prototrophy Of Hbcontrasting

confidence: 69%

Efficient management of the nitritation-anammox microbiome through intermittent aeration: absence of the NOB guild and expansion and diversity of the NOx reducing guild suggests a highly reticulated nitrogen cycle

Palomo

Touceda‐Suárez

et al. 2022

Environmental Microbiome

View full text Add to dashboard Cite

Obtaining efficient autotrophic ammonia removal (aka partial nitritation-anammox, or PNA) requires a balanced microbiome with abundant aerobic and anaerobic ammonia oxidizing bacteria and scarce nitrite oxidizing bacteria. Here, we analyzed the microbiome of an efficient PNA process that was obtained by sequential feeding and periodic aeration. The genomes of the dominant community members were inferred from metagenomes obtained over a 6 month period. Three Brocadia spp. genomes and three Nitrosomonas spp. genomes dominated the autotrophic community; no NOB genomes were retrieved. Two of the Brocadia spp. genomes lacked the genomic potential for nitrite reduction. A diverse set of heterotrophic genomes was retrieved, each with genomic potential for only a fraction of the denitrification pathway. A mutual dependency in amino acid and vitamin synthesis was noted between autotrophic and heterotrophic community members. Our analysis suggests a highly-reticulated nitrogen cycle in the examined PNA microbiome with nitric oxide exchange between the heterotrophs and the anammox guild.

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“…The relative abundance of Proteobacteria in A1 (phenol), A2 (sodium acetate), and A3 (glucose) was 37.05%, 33.17%, and 43.52%, respectively. Proteobacteria widely exist in anammox consortia and the nitrification reactors [ 30 ]. Some Proteobacteria may potentially perform SRAO (Sulfammox) [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Applications of Sponge Iron and Effects of Organic Carbon Source on Sulfate-Reducing Ammonium Oxidation Process

Zhu

Shidong

Wang

et al. 2022

IJERPH

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The typical characteristics of wastewater produced from seafood, chemical, textile, and paper industries are that it contains ammonia, sulfate, and a certain amount of chemical oxygen demand (COD). The sulfate-reducing ammonium oxidation process is a biochemical reaction that allows both ammonia and sulfate removal, but its low growth rate and harsh reaction conditions limit its practical application. Due to the adsorption properties of the iron sponge and its robust structure, it provides a suitable living environment for microorganisms. To reduce the negative impact on the environment, we employed 4.8 kg of sponge iron in a 2.0 dm3 anaerobic sequencing batch reactor (ASBR). We investigated the effects of the type and concentration of carbon sources on the performance of the sulfate-reducing ammonium oxidation (SRAO) process. The results demonstrated that during a start-up period of 90 days, the average ammonium removal efficiency and the sulfate conversion efficiency of the reactor containing the sponge iron were 4.42% and 8.37% higher than those of the reactor without the sponge iron. The addition of the sponge iron shortens the start-up time of this greenhouse gas-free denitrification process and reduces future costs in practical applications. The removal of total nitrogen (TN) significantly increased after adding organic carbon sources and then declined sharply, while the most considerable reduction of ammonium removal efficiency from 98.4% to 30.5% was observed with adding phenol. The performance of the group employing glucose as the carbon source was recovered on the 28th day, with the average ammonium removal efficiency increasing from 49.03% to 83.5%. The results of this simulation study will help the rapid start-up of SRAO in the water treatment industry and can precisely guide the application of the SRAO process for wastewater containing different organic carbon sources.

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Decoding the interspecies interaction in anammox process with inorganic feeding through metagenomic and metatranscriptomic analysis

Cited by 45 publications

References 52 publications

Efficient Management of the Nitritation-Anammox Microbiome Through Intermittent Aeration: Absence of the NOB Guild and Expansion and Diversity of the NOx Reducing Guild Suggests A Highly Reticulated Nitrogen Cycle

Efficient Management of the Nitritation-Anammox Microbiome Through Intermittent Aeration: Absence of the NOB Guild and Expansion and Diversity of the NOx Reducing Guild Suggests A Highly Reticulated Nitrogen Cycle

Efficient management of the nitritation-anammox microbiome through intermittent aeration: absence of the NOB guild and expansion and diversity of the NOx reducing guild suggests a highly reticulated nitrogen cycle

Applications of Sponge Iron and Effects of Organic Carbon Source on Sulfate-Reducing Ammonium Oxidation Process

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