Decoding the resistome, virulome and mobilome of clinical versus aquatic Acinetobacter baumannii in southern Romania

Gheorghe-Barbu, Irina; Surleac, Marius; Barbu, Ilda Czobor; Paraschiv, Simona; Bănică, Leontina Mirela; Rotaru, Liviu-Iulian; Vrâncianu, Corneliu Ovidiu; Niță Lazăr, Mihai; Oțelea, Dan; Chifiriuc, Mariana Carmen

doi:10.1016/j.heliyon.2024.e33372

Heliyon

2024

DOI: 10.1016/j.heliyon.2024.e33372

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Decoding the resistome, virulome and mobilome of clinical versus aquatic Acinetobacter baumannii in southern Romania

Irina Gheorghe-Barbu,

Marius Surleac,

Ilda Czobor Barbu

et al.

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2024

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Tracing Acinetobacter baumannii’s Journey from Hospitals to Aquatic Ecosystems

Gheorghe-Barbu,

Dragomir,

Gradisteanu Pircalabioru

et al. 2024

Microorganisms

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Background: This study provides a comprehensive analysis of Acinetobacter baumannii in aquatic environments and fish microbiota by integrating culture-dependent methods, 16S metagenomics, and antibiotic resistance profiling. Methods: A total of 83 A. baumannii isolates were recovered using culture-dependent methods from intra-hospital infections (IHI) and wastewater (WW) and surface water (SW) samples from two southern Romanian cities in August 2022. The antibiotic susceptibility was screened using disc diffusion, microdilution, PCR, and Whole Genome Sequencing assays. Results: The highest microbial load in the analyzed samples was found in Glina, Bucharest, for both WW and SW samples across all investigated phenotypes. For Bucharest isolates, the resistance levels corresponded to fluoroquinolones > aminoglycosides > β-lactam antibiotics. In contrast, A. baumannii from upstream SW samples in Târgoviște showed the highest resistance to aminoglycosides. The blaOXA-23 gene was frequently detected in IHI, WW, and SW isolates in Bucharest, but was absent in Târgoviște. Molecular phylogeny revealed the presence of ST10 in Târgoviște isolates and ST2 in Bucharest isolates, while other minor STs were not specifically correlated with a sampling point. Using 16S rRNA sequencing, significant differences in microbial populations between the two locations was identified. The low abundance of Alphaproteobacteria and Actinobacteria in both locations suggests environmental pressures or contamination events. Conclusions: These findings indicate significant fecal contamination and potential public health risks, emphasizing the need for improved water quality monitoring and management.

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Tracing Acinetobacter baumannii’s Journey from Hospitals to Aquatic Ecosystems

Gheorghe-Barbu,

Dragomir,

Gradisteanu Pircalabioru

et al. 2024

Microorganisms

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show abstract

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Decoding the resistome, virulome and mobilome of clinical versus aquatic Acinetobacter baumannii in southern Romania

Cited by 1 publication

References 63 publications

Tracing Acinetobacter baumannii’s Journey from Hospitals to Aquatic Ecosystems

Tracing Acinetobacter baumannii’s Journey from Hospitals to Aquatic Ecosystems

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