Decolorization of Azo Dye (Orange MR) by an Autochthonous Bacterium, Micrococcus sp. DBS 2

Abstract

Soil and sediment samples obtained from Orange MR dye contaminated habitat were screened for heterotrophic bacterial population. The heterotrophic bacterial density of dye-contaminated soil was 2.14 9 10 6 CFU/g. The generic composition of heterotrophic bacterial population was primarily composed of 10% of Proteus sp., 15% Aeromonas sp., 20% Bacillus sp., 25% Pseudomonas sp. and 30% Micrococcus sp. The bacterial strain that decolorized the azo dye Orange MR up to 900 ppm was identified as Micrococcus sp. The optimum inoculum load, pH and temperature were found to be 5%, 6 and 35°C, respectively. The rate of decolorization was assessed using spectrophotometer at 530 nm and the percentage of decolorization was ascertained. The autochthonous bacterial isolate was able to utilize the dye as both nitrogen and carbon source.

Fed-Batch Decolourization of Mixture of Brilliant Green and Evans Blue by Bacteria Species Applied as Pure and Mixed Cultures: Influence of Growth Conditions

“…As mentioned previously, additional carbon and nitrogen sources, and a pH value in the range of 5 to 7, generally have a positive influence on decolourization efficiency (Chen et al 2003;Meerbergen et al 2018;Moosvi et al 2005;Rajee and Patterson 2011;Sawhney and Kumar 2011;Solis et al 2012).…”

“…It is worth emphasizing that yeast extract (the growth medium component used) is considered essential to the regeneration of important factors such as NADH, which acts as an electron donor for the reduction of dye bonds. Rajee and Patterson (2011) showed that the highest efficiency of decolourization of orange MR by Micrococcus sp. (80%) was reached when pH = 6.…”

Industrial Wastewater Treatment: A Challenging Task in the Industrial Waste Management

“…The use of non-conventional, economical sources as precursors for activated carbon has been proposed to achieve the cost-effectiveness in the application. As previously stated, adsorption is a non-destructive method in which there is only the change in the phase of the removed impurities and, therefore impose further problems in the form of sludge [121][122][123][124]. The high cost also necessitates the adsorbent regeneration.…”

Environmental Bioremediation: A Low Cost Nature’s Natural Biotechnology for Environmental Clean-up

“…As the extent of decolouration is influenced by the pH of the media, pH also affects the colour of the solution and the solubility of the dye. Candida tropicalis adsorbs 45% of Basic Violet 3 at pH 3, 85% at pH 4 and 33% at pH 9 [83]. Micrococcus sp.…”