Decolorization of Malachite green dye by Stenotrophomonas maltophilia a compost bacterium

Alaya, Vasudhaudupa; Kodi, Rajesh Kumar; Ninganna, Earanna; Gowda, Balakrishna; Shivanna, M. B.

doi:10.1186/s42269-021-00518-w

Cited by 23 publications

(17 citation statements)

References 69 publications

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“…The samples of culture tubes were centrifuged at 5000 rpm for 20 min. The remaining concentration in the aliquots were determined through UV-Visible spectrophotometer [16].…”

Section: The Impact Of Ph On Dye Degradationmentioning

confidence: 99%

Biodegradation of Brown 706 Dye by Bacterial Strain Pseudomonas aeruginosa

Khan

Rehman

Zahoor

et al. 2021

Water

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Dyes are the most challenging pollutants for the aquatic environment that are not only toxic, but also interfering photosynthesis as light penetration into deep water is changed. A number of methods are used for the water reclamation, however, among them biological methods are preferably used due to their compatibility with nature. In the present research, 15 different bacterial strains were used to decolorize Brown 706 dye. Among the bacterial strains, Pseudomonas aeruginosa showed maximum decolorization activity; hence in the subsequent experiments Pseudomonas aeruginosa was used. First the decolorization activities were carried out under different physicochemical conditions to obtain the optimum decolorization benefits of the selected microorganism. The optimum conditions established were 37°C, pH of 7 and operation cycle time 72 h. In the subsequent experiment all optimum conditions were combined in a single experiment where 73.91% of decolorization efficiency was achieved. For the evaluation of metabolites formed after decolorization/degradation the aliquots containing bacteria were homogenized, filtered and then subjected to extraction. The extracted metabolites were then subjected to the silica gel column isolation. UV–Vis, FTIR, and NMR techniques were used to elucidate structures of the metabolites. Out of the collected metabolites only P-xylene was identified, which has been formed by cleavage of azo linkage by azo reductase enzyme of bacteria following the deamination and methylation of nitro substituted benzene ring.

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“…The samples of culture tubes were centrifuged at 5000 rpm for 20 min. The remaining concentration in the aliquots were determined through UV-Visible spectrophotometer [16].…”

Section: The Impact Of Ph On Dye Degradationmentioning

confidence: 99%

Biodegradation of Brown 706 Dye by Bacterial Strain Pseudomonas aeruginosa

Khan

Rehman

Zahoor

et al. 2021

Water

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“…Cheng et al also reported that bioremoval of RB5 was performed by O. anthropi with the highest efficiency at pH 7 and 9 19 . Alaya et al reported that S. maltophilia had an efficient malachite green removal yield at pH 8 21 . Johansson et al concluded that pollutant reduction efficiency was dye‐specific and pH‐dependent due to dye‐enzyme interactions 22 .…”

Section: Resultsmentioning

confidence: 99%

“…19 Alaya et al reported that S. maltophilia had an efficient malachite green removal yield at pH 8. 21 Johansson et al concluded that pollutant reduction efficiency was dye-specific and pH-dependent due to dye-enzyme interactions. 22 In this study, the highest Setazol Black B removal rate was achieved at pH 8, likely due to the pH-dependent nature of dye-enzyme interactions.…”

Section: Effect Of Ph On Bioremediation By Bacterial Consortiummentioning

confidence: 99%

Nanoremediation of toxic dyes using a bacterial consortium immobilized on cellulose acetate nanofiber mats

Erkoç,

Tüzün,

Korkmaz

et al. 2023

Polymer Engineering & Sci

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Stenotrophomonas maltophilia and Ochrobactrum sp. demonstrated the highest rates of dye bioremediation. The trials were performed at pH 8, which resulted in the highest bioremediation rate of 64.6% in media containing 21.2 mg L−1 dye. As the dye concentration increased, the pollutant removal decreased, with the maximum bioremoval rate of 70.3%. The removal capacity was increased with an increase in biomass concentration; the highest yield of 91.3% was obtained in media containing 14.2 mg L−1 dye and 12% (v/v) biomass. In nanoremediation studies, the bacterial consortium was immobilized on cellulose acetate nanofiber mats (CA‐NFM). Scanning electron microscopic micrographs showed that bead‐free nanofiber mats were effective in immobilizing bacterial cells. Moreover, nanofiber structures were capable of supporting exopolysaccharides formation, as confirmed by Fourier transform ınfrared spectroscopy. The bacterial consortium immobilized on CA‐NFM showed a maximum bioremoval rate of 56.5%. Reusability tests demonstrated that the consortium immobilized CA‐NFM could be used at least five times. Furthermore, after leaving the mat for 1 month at 4°C, it was still usable, and the removal efficiency was found to be 45.4%. Based on our findings, bacteria immobilized on CA‐NFM have the potential to be used as highly effective and versatile nanobiotechnological biological sorbents in the treatment of wastewater containing dyes.Highlights Stenotrophomonas maltophilia and Ochrobactrum sp. were used as a bacterial consortium. Bacterial consortium immobilized on cellulose acetate nanofiber mats (CA‐NFM) was tested. EPS production by the consortium was confirmed by Fourier transform ınfrared spectroscopy analysis. CA‐NFM could be used at least five times.

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“…We believe that the present study is one of the few reports that deal with the screening of bacterial laccases as far as our knowledge. As a result of a literature survey, it was shown that some S. maltophilia E1 strains had been implicated in the production of laccase (Galai et al 2009 ; Alaya et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes

Albulaihed,

Adnan,

Jamal

et al. 2023

Bioresour. Bioprocess.

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Enzymatic degradation of synthetic dyes holds an immense promise for addressing the environmental concerns associated with the textile and dye industries. This study aimed to isolate bacteria capable of producing laccase enzymes from an anthropogenic environment. Subsequently, viability of utilizing cost-effective agricultural residues as substrates for laccase production was assessed. Response Surface Methodology (RSM) and the One Variable at a Time (OVAT) approach was pursued for the optimization of laccase production, followed by pH and temperature stability, dye degradation and decolorization experiments, toxicological studies on the degraded dye metabolites. In results, laccase-producing bacterial strain was identified as Stenotrophomonas maltophilia strain E1 (S. maltophilia). Among variety of substrates, coconut husk exhibited optimal efficacy. In a statistical optimization study, it was found that S. maltophilia was capable of producing laccase 51.38 IU/mL, i.e., three times higher than the amount of laccase produced by unoptimized medium (16.7 IU/mL), and the enzyme activity was found to be steady at an acidic pH, and a mesophilic temperature range. The laccase obtained from S. maltophilia E1 demonstrated proficient dye decolorization capabilities, achieving a notable 92.1% reduction in Malachite green dye coloration at a concentration of 500 ppm. Gas chromatography–mass spectrometry (GC–MS) analysis of the decolorized derivatives of Malachite green revealed a conversion into a distinct compounds. Moreover, after undergoing laccase treatment, Malachite green exhibited decreased phytotoxic effects on Oryza sativa, pointing to enzymatic detoxification. Collectively, insights gained from the present study will contribute to the development of efficient enzymatic approaches for addressing the environmental pollution caused by synthetic dyes. Graphical Abstract

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Decolorization of Malachite green dye by Stenotrophomonas maltophilia a compost bacterium

Cited by 23 publications

References 69 publications

Biodegradation of Brown 706 Dye by Bacterial Strain Pseudomonas aeruginosa

Biodegradation of Brown 706 Dye by Bacterial Strain Pseudomonas aeruginosa

Nanoremediation of toxic dyes using a bacterial consortium immobilized on cellulose acetate nanofiber mats

Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes

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