This work proposes a novel technique for biological compound transformation by a circulating electrical breakdown in the liquid (EBL) process. Regarding the experimental results, it could be confirmed that the EBL process has the potential to depolymerize the inulin structure. The EBL process has been operated at 50Hz positive half wave 4.32 kV supplied voltage. After 20 min EBL process of one gram of inulin dissolved in 100 ml of deionized water, the inulin structures have been deformed from a 20-100 µm micro spherical shape to small flakes. Moreover, the DPPH analysis results show that the antioxidant activity and prebiotic activity of inulin have been improved by 311%, and 35%, respectively.