Decolorization of sulfonphthalein dyes by manganese peroxidase activity of the white-rot fungusPhanerochaete chrysosporium

Abstract

Manganese peroxidase (MnP) was produced by shallow stationary cultures of Phanerochaete chrysosporium growing on N-limited medium. Decolorization of sulfonphthalein (SP) dyes by MnP was investigated. The MnP activity profile and decolorization of SP dyes was correlated and almost all dyes were decolorized at pH 4.0. The influence of various inhibitors on Bromocresol Purple decolorization suggested an oxidative nature of the MnP-catalyzed decolorization of SP dyes.

Peroxidase mediated decolorization and remediation of wastewater containing industrial dyes: a review

“…The order of preference for SP dyes as substrate for MnP was Phenol Red (Christian et al 2003;Shrivastava et al 2005). Harazono and Nakamura (2005) investigated the decolorization of mixtures of four reactive textile dyes, including azo and anthraquinone dyes, by a white-rot basidiomycete Phanerochaete sordida.…”

Molecular Characterization of a Novel Peroxidase from the Cyanobacterium Anabaena sp. Strain PCC 7120

“…There are several reports of microorganisms capable of decolorizing synthetic dyes. This has been attributed to their growth and production of enzymes such as laccase (1,9,40), azoreductases (3), and peroxidases, for example, lignin peroxidase (12,25,36), manganese peroxidase (10,38), and versatile peroxidase (16). However, most of the synthetic dyes are xenobiotic compounds that are poorly degraded using the typical biological aerobic treatments.…”

“…They are one of the most studied enzymes because of their inherent spectroscopic properties and potential use in both diagnostic and bioindustrial applications. In particular, their ability to degrade a wide range of substrates has recently stimulated interest in their potential application in environmental bioremediation of recalcitrant and xenobiotic wastes (10,25,26).…”

Functional Suitability of Soluble Peroxidases from Easily Available Plant Sources in Decolorization of Synthetic Dyes

“…However, the decolorization of textile and other dyes by BGP in presence of 1.0 mM HOBT appeared without the formation of any precipitate. It suggested that the decolorization of dyes took place via degradation of aromatic ring of the compounds or by cleaving certain functional groups as reported elsewhere (Christian et al, 2003;Husain et al, 2009). HOBT could have a dual role, first as a mediator by increasing the substrate range of dyes for BGP and second enhancing the rate of oxidation.…”