Decolorization of textile azo dye Novacron Red using bacterial monoculture and consortium: Response surface methodology optimization

Abstract

The present study was intended toward the optimization of a textile dye Novacron Red decolorization by single and mixed culture of Bacillus strains namely, B. firmus, B. filamentosus and B. subterraneus. Optimization of dye decolorization using Bacillus monocultures was conducted using central composite design. The maximum dye decolorization achieved under optimized conditions for B. firmus, B. filamentosus and B. subterraneus was 89.24%, 88.28% and 88.45%, respectively. The effect of various consortia of selected Bacillus strains on dye removal was evaluated by applying a mixture design. The best dye (100 mg/L) decolorization yield (84%) was achieved using the consortium of B. filamentosus and B. subetrraneus.The Fourier Transform Infrared Spectroscopy analyses confirmed biodegradation potential of the two Bacillus strains. The results highlighted the potential of mono‐ and co‐cultures of Bacillus strains for application in textile wastewater treatment. Practitioner points Novel dye‐decolorizing Bacillus strains were isolated from marine sediment. Optimization of decolorization was conducted using response surface methodology. Efficient decolorization of textile dye by Bacillus strains on mono‐ and co‐cultures. The efficiency of the consortium B. filamentosus and B. subetrraneus on dye removal.

Biodegradation and Decolorization of Crystal Violet Dye by Cocultivation with Fungi and Bacteria

“…However, the study discovered that the strains in the coculture method lacked the potential to efficiently exert their synergistic impact in increasing enzyme production. Furthermore, some researchers have suggested that single strains may be a more preferable option for dye decolorization compared to mixed cultures. , …”

“…Furthermore, some researchers have suggested that single strains may be a more preferable option for dye decolorization compared to mixed cultures. 40,6 2.4. Enzyme Activity in Dye Decolorization.…”

“…The discharge of textile dye wastewater can pose serious health risks to the ecosystem, and unfortunately, a considerable amount (10–15%) of dye wastewater is directly discharged without any treatment . Synthetic dyes, which are commonly used in the textile industry, are highly resistant to light, temperature, and microbial attack, making it challenging to decolorize them. , A number of methods, including biodegradation, adsorption, photocatalytic degradation, electro-Fenton process, and advanced oxidation processes (AOPs), have been attempted to degrade synthetic dyes, such as CV . However, it has been found that physical or chemical treatment methods, such as adsorption, precipitation, chemical degradation, and photodegradation, are costly, inefficient, and environmentally unfriendly.…”

Response Surface Optimization of Vat Blue 4 Degradation Process Using <i>Pseudomonas aeruginosa</i> WYT

“…The optimization of culture conditions is important for the development of any biotreatment processes for removing dyes contamination [23,24]. To explore the effect of different factors on Pseudomonas aeruginosa WYT degradation of VB4, single colonies of VB4 discoloration were inoculated and grown in 50 mL flasks containing 10 mL of LB medium at 30ºC and 200 rpm.…”

Whole genome sequencing and comparative genomic studies ofPriestia filamentosaJURBA-X for its drought-tolerance, plant-growth promotion, and fluorescent characteristics

“…Earlier, B. subtilis (K7), B. filamentosus (K1) and Lysinibacillus cresolivorans (K5) were isolated from desert soil samples of Cholistan, Pakistan and several thermophilic proteases isolated from B. filamentosus (K1) show optimum temp at 65°C, whereas these proteases can de-stain the blood from cotton cloth and de-hair the cow’s skin (Cresolivorans et al, 2021). The monoculture and consortium of B. filamentosus, B. firmus and B. subterraneus show decolorization of Novacron Red textile dye (Guembri et al, 2021). These studies show the use of B. filamentosus , the constitution of this consortia, and its potential application in textile water treatment.…”