Decolorization of the azo dye Acid Orange 51 by laccase produced in solid culture of a newly isolated Trametes trogii strain

Daâssi, Dalel; Zouari-Mechichi, Héla; Frikha, Fakher; Martínez, Miguel Ángel Herráiz; Mechichi, Tahar

doi:10.1007/s13205-012-0076-2

Cited by 29 publications

(24 citation statements)

References 42 publications

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“…Eventually, low concentration of laccase (5 U/ml) was also able to achieve higher decolorization percentage which was comparable to earlier reports . Similar studies of Dassai et al gave 87.87% decolorization of acid orange 51 azo dye in 48 hr using Box–Behnken design . The findings are also in good agreement with the reports of Zhang et al wherein >80% decolorization of a wide range of dyes have been reported using same quantity of laccase .…”

Section: Resultssupporting

confidence: 90%

“…19 Several chemical and physical methods are available for such detoxification of dyes through removal, adsorption, coagulation, ozonation, electrochemical destruction, membrane filtrations, ion exchange, and irradiation. 20,21 Most of these methods are also associated with disadvantages such as high operational cost, long retention time, formation of undesirable and toxic by-products like aromatic amines. [21][22][23] This necessitate addressing problems related to pollution with more eco-friendly process.…”

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confidence: 99%

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Bioprocess optimization of laccase production through solid substrate fermentation using Perenniporia tephropora‐L168 and its application in bioremediation of triaryl‐methane dye

Patil¹,

Chopade

Narkhede³

et al. 2019

Biotechnology Progress

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Laccases are multi copper oxidases that can oxidize both phenolic and nonphenolic lignin related compounds. Consequently, there has been continuous demand for laccases for the oxidative degradation of phenolic dyes in effluents. In view of this, the present work was focused on laccase production by solid substrate fermentation using a newly isolated fungus Perenniporia tephropora‐L168. To intensify the laccase production, the process parameters pH, nitrogen, inducer, and substrate: water ratio were optimized by using statistical model. A set of optimal conditions noted were pH 3, nitrogen 0.001 g/L; inducer 0.5% and substrate: water ratio (1:10), which yielded laccase 1,160 U/g. The crude laccase exhibited noteworthy potential to degrade a triaryl‐methane dye especially Malachite green. Also, during bioremediation studies, the statistical process optimization could achieve 81% decolourization within 180 min. The laccase treatment brought chemical transformation in malachite green as evident from UV–Visible spectra, FTIR, HPLC while toxicity against bacteria and fungi was also reduced. During phytotoxicity study, effect of treated and untreated dye on germination of seed was analyzed. Interestingly, the germination index for Vigna aconitifolia and Vigna radiata was increased by two and fourfold, respectively. Overall, this work demonstrates optimized production of laccase using Perenniporia tephropora‐L168 and its efficient bioremediation potential for triaryl‐methane dye.

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Section: Resultssupporting

confidence: 90%

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confidence: 99%

Bioprocess optimization of laccase production through solid substrate fermentation using Perenniporia tephropora‐L168 and its application in bioremediation of triaryl‐methane dye

Patil¹,

Chopade

Narkhede³

et al. 2019

Biotechnology Progress

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“…These molecules are chemically stable and difficult to biodegrade. Several studies reported the toxicity of RB-5 [36] and AO51 [37]. …”

Section: Resultsmentioning

confidence: 99%

“…SSF using sawdust can be a suitable process for RB-5 decolorization. Several studies reported that the azo dye RB-5 is difficult to biodegrade and needs a mediator redox in the case of enzymatic decolorization [36, 37]. …”

Section: Resultsmentioning

confidence: 99%

Sawdust waste as a low-cost support-substrate for laccases production and adsorbent for azo dyes decolorization

Daâssi

Zouari-Mechichi

Frikha

et al. 2016

J Environ Health Sci Engineer

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BackgroundLaccases are multicopper oxidases with high potential for environmental and industrial applications. Low-cost laccase production could be achieved by solid state fermentation on agro-industrial by-products.MethodsA number of agro-industrial solid wastes were tested as support-substrate for laccase production by Coriolopsis gallica under solid-state fermentation (SSF) conditions. Response surface methodology (RSM) was used to optimize the medium composition for laccase production. Initial screening by Plackett-Burman design was performed to select the major variables out of 20 tow medium components fellowing this Central composite design (CCD) was employed to optimize the level of the selected variables.ResultsSawdust waste was shown to be the best support-substrate for laccase production by the C. gallica. Peptone as source of organic nitrogen, Cd2+ as laccase inducer and liquid/solid (L/S) ratio were found to have significant effects on laccase production. Operating at optimum concentrations of the most significant variables (peptone, 4.5 g L−1, L/S ratio, 5.0 and Cd+2 1.0 mM) extracellular laccase activity was enhanced from 1480 U L−1 (60.5 U g−1), to 4880 U L−1 (200 U g−1) which meant a 3.2-fold increase in laccase activity. On the other hand, sawdust waste was studied as a low cost adsorbent to remove the azo dyes Reactive Black 5 (RB5) and Acid Orange 51 (AO51). Decolorization percentages around 67 and 75 % were obtained in 24 h for RB5 and AO51, respectively.ConclusionWhen used as a support substrate, sawdust yielded the highest laccase production which was increased 3.2 times using RMS optimization.

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“…These enzymes degrade azo dyes through a highly nonspecific free radical mechanism to form phenolic compounds, thereby avoiding the formation of toxic aromatic amines as the final products (Mirzadeh et al 2014;Mohajershojaei et al 2014). Decolorization of synthetic and textile dyes using free laccases produced by white rot fungi has been investigated in a number of studies, many of which exhibited efficiency between 80 and 90%, for example, the decolorization of Acid Orange 51 (87.87%) by crude laccase from Trametes trogii (Daâssi et al 2013), Reactive Black 5 (87.07%) by crude laccase from Trametes gibbosa sp. (Adnan et al 2014), and Malachite green (89.88%) by laccase produced from Cerrena sp.…”

Section: Introductionmentioning

confidence: 99%

A Novel Xylan-Polyvinyl Alcohol Hydrogel Bead with Laccase Entrapment for Decolorization of Reactive Black 5

Bankeeree¹,

Prasongsuk²,

Imai³

et al. 2016

BioResources

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In an attempt to find a more efficient technique for biodegradation of the recalcitrant Reactive Black 5 (RB-5) dye, a composite xylan-polyvinyl alcohol (xylan-PVOH) hydrogel was used to immobilize laccase from the white-rot fungus Trametes versicolor. Xylan was prepared from the black liquor of pulp and paper effluent, and it was esterified with citric acid prior to cross-linking with polyvinyl alcohol (PVOH). The optimum composition for the immobilized laccase bead formation consisted of 4% (w/v) modified xylan, 10% (w/v) PVOH, and 15 U.mL -1 crude laccase. The maximum decolorization of RB-5 (98.45  1.96 %) was obtained within the first cycle (6 h) at 40 °C. In the eighth cycle, the reused beads were able to decolorize 55.35  2.46 % of the RB-5. Moreover, the xylan-PVOH beads extended the optimum pH range of laccase activity from 6 to 10 and tolerated a temperature up to 10 °C higher than that of the free enzyme. These results suggest that the xylan-PVOH bead has great potential as the polymer matrix for enzyme immobilization, which has applications in wastewater treatment.

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Decolorization of the azo dye Acid Orange 51 by laccase produced in solid culture of a newly isolated Trametes trogii strain

Cited by 29 publications

References 42 publications

Bioprocess optimization of laccase production through solid substrate fermentation using Perenniporia tephropora‐L168 and its application in bioremediation of triaryl‐methane dye

Bioprocess optimization of laccase production through solid substrate fermentation using Perenniporia tephropora‐L168 and its application in bioremediation of triaryl‐methane dye

Sawdust waste as a low-cost support-substrate for laccases production and adsorbent for azo dyes decolorization

A Novel Xylan-Polyvinyl Alcohol Hydrogel Bead with Laccase Entrapment for Decolorization of Reactive Black 5

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