Pleurotus fungi are one of the biotechnologically treasured fungi may also be known as oyster or tree mushrooms. Pleurotus ostreatus is a widely used oyster mushroom. Edible mushrooms of this category are generally known for their significant roles in the various field of biotechnology like in food industries, bioremediation, enzyme production, medicinal biotechnology, bioengineering and so on. They have various biotechnologically valuable applications as promising bioremediation, anti-diabetic, anti-inflammatory, anti-cancerous, anti-microbial, anti-oxidant, and nematocidal and many more. This short review describes about the recent studies (year 2020) on the biotechnological applications of Pleurotus spp.