Decolourisation of Acid orange 7 in a microbial fuel cell with a laccase-based biocathode: Influence of mitigating pH changes in the cathode chamber

Mani, Priyadharshini; Keshavarz, T.; Chandra, T.S.; Kyazze, Godfrey

doi:10.1016/j.enzmictec.2016.10.012

Cited by 61 publications

(26 citation statements)

References 32 publications

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“…The laccase-based biocathode MFC (lbMFC) produced the maximum open circuit voltage of 250 mV, output voltage of 145 mV (with a 1,000 Ω resistor), power density of 59 mW/m , contributing to the degradation of recalcitrant aromatic compounds [4,5]. In previous studies with the fungal enzyme immobilized on the surface of the cathode, laccase obtained from fungi like Ganodium lucidum strain BCRC 36123, Trametes versicolor, and Pleurotus ostreatus has successfully enhanced the generation of electricity [6][7][8][9][10]. Nevertheless, the enzymes commercially available from the fungal sources and others are quite expensive, as they are produced via a laborious purification process.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Electricity Generation Using a Laccase-Based Microbial Fuel Cell with Yeast Galactomyces reessii on the Cathode

Chaijak¹,

Sukkasem²,

Lertworapreecha³

et al. 2018

Journal of Microbiology and Biotechnology

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Section: Introductionmentioning

confidence: 99%

Enhancing Electricity Generation Using a Laccase-Based Microbial Fuel Cell with Yeast Galactomyces reessii on the Cathode

Chaijak¹,

Sukkasem²,

Lertworapreecha³

et al. 2018

Journal of Microbiology and Biotechnology

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“…, acidification at the anode can decrease bacterial activity and affect biofilm stability and performance. Most studies have shown that a pH ranging from 6 to 9 is suitable for growth and operation of biofilms derived from pH‐neutral wastewater . As pH values differ in both chambers of the MFC, this parameter is crucial to the output power generated from MFC.…”

Section: Operating Principle Of Microbial Fuel Cellmentioning

confidence: 99%

“…Carbon dioxide (CO 2 ) can be used in the cathode as it can combine with the hydroxide ions and create a carbonate or bicarbonate buffered catholyte system. Some studies have implemented this process and found an increase in power density and cell voltage with a decreased pH imbalance …”

Section: Operating Principle Of Microbial Fuel Cellmentioning

confidence: 99%

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Sustainable power generation from wastewater sources using Microbial Fuel Cell

Bose

Gopinath

Parthasarthy

2018

Biofuels Bioprod Bioref

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Microbial fuel‐cell performance depends primarily on five factors: the nature of the electrodes, pH, concentration, temperature, and period of operation. The present work describes work on optimization that has resulted in improved system performance of processes for energy recovery from wastewater by addressing these five parameters. This optimization is related to Monod kinetics, which forms the basis for microbial growth and substrate depletion rate. A difference in energy recovery from wastewater sources has been reported for studies with pure microbial culture and with undefined mixed microbes. Energy utilization research with microbial reactors has grown significantly with varying electrogenic reactor configurations, reductions in material costs, and a global need for power with reduced net CO2 emissions. The potential for future developments of these electrogenic reactor systems is also discussed, including how these systems can be integrated with existing wastewater treatment sources such as anaerobic digesters, and the positive impact they can have on energy security, which is linked with economic stability. Treatment of industrial and domestic wastewater using the microbial reserves can contribute significantly to advancing wastewater treatment infrastructure through effective COD (Chemical Oxygen Demand) removal, and in the process generate value‐added product in the form of bioelectricity. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…It is probably for this reason that high enzyme loadings such as 500 U mL −1 to 2000 U mL −1 are used in various dye decolorizing experiments [16,17]. Therefore methods to decrease the enzyme loading and reduce the cost of mediators should be further examined [18]. In this study natural mediators such as syringaldehyde and acetosyringone were studied with relatively low enzyme loadings (300 U L −1 ) with a view to developing a low cost and sustainable laccase-mediator system.…”

Section: Introductionmentioning

confidence: 99%

The Role of Natural Laccase Redox Mediators in Simultaneous Dye Decolorization and Power Production in Microbial Fuel Cells

et al. 2018

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Redox mediators could be used to improve the efficiency of microbial fuel cells (MFCs) by enhancing electron transfer rates and decreasing charge transfer resistance at electrodes. However, many artificial redox mediators are expensive and/or toxic. In this study, laccase enzyme was employed as a biocathode of MFCs in the presence of two natural redox mediators (syringaldehyde (Syr) and acetosyringone (As)), and for comparison, a commonly-used artificial mediator 2,2 -azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) was used to investigate their influence on azo dye decolorization and power production. The redox properties of the mediator-laccase systems were studied by cyclic voltammetry. The presence of ABTS and As increased power density from 54.7 ± 3.5 mW m −2 (control) to 77.2 ± 4.2 mW m −2 and 62.5 ± 3.7 mW m −2 respectively. The power decreased to 23.2 ± 2.1 mW m −2 for laccase with Syr. The cathodic decolorization of Acid orange 7 (AO7) by laccase indicated a 12-16% increase in decolorization efficiency with addition of mediators; and the Laccase-Acetosyringone system was the fastest, with 94% of original dye (100 mgL −1 ) decolorized within 24 h. Electrochemical analysis to determine the redox properties of the mediators revealed that syringaldehyde did not produce any redox peaks, inferring that it was oxidized by laccase to other products, making it unavailable as a mediator, while acetosyringone and ABTS revealed two redox couples demonstrating the redox mediator properties of these compounds. Thus, acetosyringone served as an efficient natural redox mediator for laccase, aiding in increasing the rate of dye decolorization and power production in MFCs. Taken together, the results suggest that natural laccase redox mediators could have the potential to improve dye decolorization and power density in microbial fuel cells. Energies 2018, 11, 3455 2 of 12The enzyme is widely utilised in the oxidation of phenolic and non-phenolic substrates such as dyes, pesticides, antibiotics etc.The redox potential of the substrate should be lower than that of laccase for oxidation to be thermodynamically feasible. The redox potential range for fungal laccase is between 0.4-0.8 V vs. standard hydrogen electrode (SHE), which is suitable for oxidation of phenolic substrates; for non-phenolic substrates that have a redox potential of >1.3 V vs. SHE, and cannot be oxidized directly by laccase, a redox mediator is required [5]. A redox mediator is a small molecular weight compound that is oxidized by the enzyme and reduced by the substrate continuously. They act as electron shuttles for large substrates that cannot access the active site of the enzyme, e.g., due to steric hindrance [6]. In laccase mediator systems (LMS), the enzyme oxidizes the mediators to form stable radicals with high redox potential that diffuse away from the enzyme active site and oxidize the substrates and get reduced in the process. In this way, laccase indirectly oxidises substrates that have high redox potentials or large molecular sizes [7] (...

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Decolourisation of Acid orange 7 in a microbial fuel cell with a laccase-based biocathode: Influence of mitigating pH changes in the cathode chamber

Cited by 61 publications

References 32 publications

Enhancing Electricity Generation Using a Laccase-Based Microbial Fuel Cell with Yeast Galactomyces reessii on the Cathode

Enhancing Electricity Generation Using a Laccase-Based Microbial Fuel Cell with Yeast Galactomyces reessii on the Cathode

Sustainable power generation from wastewater sources using Microbial Fuel Cell

The Role of Natural Laccase Redox Mediators in Simultaneous Dye Decolorization and Power Production in Microbial Fuel Cells

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