Decolourization of Diazo Evans Blue by Two Strains of Pseudomonas fluorescens Isolated from Different Wastewater Treatment Plants

Abstract

face water cause harmful environmental effects. The aim of the present study was evaluation of effectiveness of diazo Evans blue decolourization by two Pseudomonas strains and estimation of process byproducts toxicity. In static conditions, both tested strains removed more than 85 % of dye after 48 h and completely decolorized samples after 120 h. Agitation had negative impact on Evans blue removal (less than 70 % of dye removed after 120 h). Ecotoxicological effects were different for both studied strains beside comparable decolourization effectiveness. Increase of zootoxicity was noticed for strain Sz6 and decrease from IV to III class was noticed for strain SDz3. Optimization of process conditions for the most promising strain SDz3 should be deeply examined.

Decolourisation of Different Dyes by two Pseudomonas Strains Under Various Growth Conditions

“…a <0.4IBG18.69 ± 3.15.35III111.11 ± 7.510.9IIMix47.62 ± 3.72.1III1.56 ± 0.4764.1IVSemistaticEB b 37.04 ± 2.802.7IIIn.d. a <0.4IBG18.69 ± 0.175.35III111.11 ± 5.660.9IIMix11.49 ± 2.28.7III1.56 ± 0.2364.1IVShakenEB b 18.87 ± 3.205.3III76.92 ± 8.301.3IIIBG9.43 ± 0.7610.6IV35.71 ± 2.132.8IIIMix7.81 ± 0.1612.8IV1.56 ± 0.1164.1IVDead biomassEB b 10.64 ± 1.809.4III24.39 ± 9.504.1IIIBG1.76 ± 0.1256.7IV3.07 ± 0.6732.6IVMix52.63 ± 5.141.9III1.56 ± 0.2264.1IVControlsEB b 9.43 ± 0.2210.6IV22.22 ± 2.104.5IIIBG0.98 ± 0.07102V3.07 ± 0.5232.6IVMix0.92 ± 0.15108.7V1.2 ± 0.0183.3IV a Not detected b Results of toxicity of Evans blue decolourization end-products were published in Zabłocka-Godlewska et al 2012 …”

“… a Not detected b Results of toxicity of Evans blue decolourization end-products were published in Zabłocka-Godlewska et al 2012 …”

“…Both selected strains were classified by the API 20NE test (Biomerieux) as Pseudomonas fluorescens (Zabłocka-Godlewska et al 2012). …”

Effectiveness of Dyes Removal by Mixed Fungal Cultures and Toxicity of Their Metabolites

“…Differences between results reached on dead and living biomass are related to changes of biomass properties after autoclaving. It is necessary to remember that thermal processes change physicochemical properties of biological material and have an influence on sorption properties (Zabłocka-Godlewska et al 2012). …”

“…These organisms are used as sorbents and/or enzymes producers for biodegradation/biotransformation. Most of studies are concentrated on dyes removal by single fungal strains (Banat et al 1996; Azmi et al 1998; Kumar Sani and Chand Banerjee 1999; Swamy and Ramsay 1999; Pointing and Vrijmoed 2000; Padamavathy et al 2003; Forgacs et al 2004; Deng et al 2008; Przystaś et al 2009; Zabłocka-Godlewska et al 2012; Kwang-Soo 2004; Revankar and Lele 2007; Knapp et al 1995; Wesenberg et al 2003; Novotny et al 2004; Gill et al 2001; Reddy 1995). Significantly fewer studies are concentrated on decolorization by mixed fungal cultures (Ramalingam et al 2010; Nascimento et al 2011; Asgher et al 2012).…”