The constructed wetland integrated with microbial fuel cell (CW-MFC) has gained attention in wastewater treatment and electricity generation owing to its electricity generation and xenobiotic removal efficiencies. This study aims to use the CW-MFC with different macrophytes for domestic wastewater treatment and simultaneously electricity generation without chemical addition. The various macrophytes such as Crinum asiaticum, Canna indica, Hanguana malayana, Philodendron erubescens, and Dieffenbachia seguine were used as a cathodic biocatalyst. The electrochemical properties such as half-cell potential and power density were determined. For wastewater treatment, the chemical oxygen demand (COD) and other chemical compositions were measured. The results of electrochemical properties showed that the maximal half-cell potential was achieved from the macrophyte D. seguine. While the maximal power output of 5.42±0.17 mW/m 2 (7.75±0.24 mW/m 3 ) was gained from the CW-MFC with D. seguine cathode. Moreover, this CW-MFC was able to remove COD, ammonia, nitrate, nitrite, and phosphate of 94.00±0.05%, 64.31±0.20%, 50.02±0.10%, 48.00±0.30%, and 42.05±0.10% respectively. This study gained new knowledge about using CW-MFC planted with the macrophyte D. seguine for domestic wastewater treatment and generation of electrical power as a by-product without xenobiotic discharge.