Deconstructing the winding path to the recapitulation of mammalian oogenesis ex vivo

Albertini, David F.; Telfer, Evelyn E.

doi:10.1073/pnas.1610646113

Cited by 6 publications

(4 citation statements)

References 19 publications

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“…General consensus exists among reproductive biologists that a mammalian ovary has finite number of follicles at birth that get depleted over time and a sudden depletion of follicles with age leads to menopause [ 1 ]. Several groups have reported ovarian stem cells (OSCs) in adult mammalian ovary that are expected to undergo neo-oogenesis and primordial follicle assembly just like spermatogenesis in testis and menopause occurs probably due to compromised somatic niche with age that becomes unable to support normal differentiation of stem cells and primordial follicle assembly [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Further characterization of adult sheep ovarian stem cells and their involvement in neo-oogenesis and follicle assembly

2018

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BackgroundStem cells in the ovary comprise of two distinct populations including very small embryonic-like stem cells (VSELs) and slightly bigger progenitors termed ovarian stem cells (OSCs). They are lodged in ovary surface epithelium (OSE) and are expected to undergo neo-oogenesis and primordial follicle (PF) assembly in adult ovaries. The ovarian stem cells express follicle stimulating hormone (FSH) receptors and are directly activated by FSH resulting in formation of germ cell nests (GCN) in vitro. Present study was undertaken to further characterize adult sheep OSCs and to understand their role during neo-oogenesis and PF assembly.MethodsStem cells were collected by gently scraping the OSE cells and were characterized by H&E staining, immuno-localization, immuno-phenotyping and RT-PCR studies. Expression of FSH receptors and markers specific for stem cells (OCT-4, SSEA-4) and proliferation (PCNA) were studied on stem/progenitor cells in OSE culture and on adult sheep ovarian cortical tissue sections. Effect of FSH on stem cells was also studied in vitro. Asymmetric cell division (ACD) was monitored by studying expression of OCT-4 and NUMB.ResultsAdditional evidence was generated on the presence of two populations of stem cells in the OSE including VSELs and OSCs. FSHR expression was observed on both VSELs and OSCs by immuno-localization and immuno-phenotyping studies. FSH treatment in vitro stimulated VSELs that underwent ACD to self-renew and give rise to OSCs which divided rapidly by symmetric cell divisions (SCD) and clonal expansion with incomplete cytokinesis to form GCN. ACD was further confirmed by differential expression of OCT-4 in VSELs and NUMB in the OSCs. Immuno-histochemical expression of OCT-4, PCNA and FSHR was noted on stem cells located in the OSE in sheep ovarian sections. GCN and cohort of PF were observed in the ovarian cortex and provided evidence in support of neo-oogenesis from the stem cells.ConclusionResults of present study provide further evidence in support of two stem cells populations in adult sheep ovary. Both VSELs, OSCs and GCN express FSH receptors and FSH possibly regulates their function to undergo neo-oogenesis and primordial follicle assembly.Electronic supplementary materialThe online version of this article (10.1186/s13048-017-0377-5) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Further characterization of adult sheep ovarian stem cells and their involvement in neo-oogenesis and follicle assembly

2018

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“…In the next decade, such a process is likely to radically change patient perspectives about their fertility potential into advancing age, once they can be given the ability to produce autologous sperm or eggs at all ages in vitro. This gametic production would also revolutionize how fertility services are currently offered to infertile patients and would allow same-sex couples conception with shared genetic parenthood 19 . In the mouse, male and female gametes have already been successfully produced from stem cells 20 , and multiple laboratories around the world are currently trying to repeat this accomplishment in humans.…”

Section: A New Horizon For Reproductive Medicine Induced By Basic Researchmentioning

confidence: 99%

How will our understanding of human development evolve over the next 10 years

2021

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In the next 10 years, the continued exploration of human embryology holds promise to revolutionize regenerative and reproductive medicine with important societal consequences. In this Comment we speculate on the evolution of recent advances made and describe emerging technologies for basic research, their potential clinical applications, and, importantly, the ethical frameworks in which they must be considered.

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“…During its genesis and differentiation process, the female oocyte coordinates appropriate chromosome segregation and ooplasm and molecular organisation (cytoplasmic maturation). Although the latter process is poorly understood, it is known to involve a physical growth of the oocyte, provided by the active synthesis of macromolecules, proteins and stage-specific and timely messenger RNAs (mRNA) and their specific posttranslational modification [ 1 ]. mRNAs work as templates of genes which move from the nucleus of the cell to the cytoplasm, where they will be translated to a series of amino acids to subsequently form proteins.…”

Section: Introductionmentioning

confidence: 99%

Systematic review of mRNA expression in human oocytes: understanding the molecular mechanisms underlying oocyte competence

Gonzalez

Almutlaq

Gupta

2023

J Assist Reprod Genet

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The biggest cell in the human body, the oocyte, encloses almost the complete machinery to start life. Despite all the research performed to date, defining oocyte quality is still a major goal of reproductive science. It is the consensus that mature oocytes are transcriptionally silent although, during their growth, the cell goes through stages of active transcription and translation, which will endow the oocyte with the competence to undergo nuclear maturation, and the oocyte and embryo to initiate timely translation before the embryonic genome is fully activated (cytoplasmic maturation). A systematic search was conducted across three electronic databases and the literature was critically appraised using the KMET score system. The aim was to identify quantitative differences in transcriptome of human oocytes that may link to patient demographics that could affect oocyte competence. Data was analysed following the principles of thematic analysis. Differences in the transcriptome were identified with respect to age or pathological conditions and affected chromosome mis segregation, perturbations of the nuclear envelope, premature maturation, and alterations in metabolic pathways—amongst others—in human oocytes.

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Deconstructing the winding path to the recapitulation of mammalian oogenesis ex vivo

Cited by 6 publications

References 19 publications

Further characterization of adult sheep ovarian stem cells and their involvement in neo-oogenesis and follicle assembly

Further characterization of adult sheep ovarian stem cells and their involvement in neo-oogenesis and follicle assembly

How will our understanding of human development evolve over the next 10 years

Systematic review of mRNA expression in human oocytes: understanding the molecular mechanisms underlying oocyte competence

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