Decontamination

Hawley, Robert J.; Kozlovac, Joseph P.

doi:10.1385/1-59259-764-5:333

Cited by 8 publications

(5 citation statements)

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“…Our oxidative stress model is based on the photocatalytic activity of the Ag-TiO 2 particles 31,35 . During the process of photocatalysis under appropriate (exciting) wavelength, reactive hydroxyl radicals (OH·) are produced, which are primarily responsible for photooxidation of organic materials or inactivating bacteria 36 . Hydroxyl radicals are the most reactive oxygen species and cause irreversible DNA damages which could lead to DNA degradation in bacteria 36 .…”

Section: Resultsmentioning

confidence: 99%

“…During the process of photocatalysis under appropriate (exciting) wavelength, reactive hydroxyl radicals (OH·) are produced, which are primarily responsible for photooxidation of organic materials or inactivating bacteria 36 . Hydroxyl radicals are the most reactive oxygen species and cause irreversible DNA damages which could lead to DNA degradation in bacteria 36 . In our previous work, the amount of reactive hydroxyl radicals formed on Ag-TiO 2 particles was determined by the hydrogen peroxide-induced luminol-dependent chemiluminescence reaction 30 .…”

Section: Resultsmentioning

confidence: 99%

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Small extracellular vesicles convey the stress-induced adaptive responses of melanoma cells

Harmati

Gyukity-Sebestyen

Dobra

et al. 2019

Sci Rep

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Exosomes are small extracellular vesicles (sEVs), playing a crucial role in the intercellular communication in physiological as well as pathological processes. Here, we aimed to study whether the melanoma-derived sEV-mediated communication could adapt to microenvironmental stresses. We compared B16F1 cell-derived sEVs released under normal and stress conditions, including cytostatic, heat and oxidative stress. The miRNome and proteome showed substantial differences across the sEV groups and bioinformatics analysis of the obtained data by the Ingenuity Pathway Analysis also revealed significant functional differences. The in silico predicted functional alterations of sEVs were validated by in vitro assays. For instance, melanoma-derived sEVs elicited by oxidative stress increased Ki-67 expression of mesenchymal stem cells (MSCs); cytostatic stress-resulted sEVs facilitated melanoma cell migration; all sEV groups supported microtissue generation of MSC-B16F1 co-cultures in a 3D tumour matrix model. Based on this study, we concluded that (i) molecular patterns of tumour-derived sEVs, dictated by the microenvironmental conditions, resulted in specific response patterns in the recipient cells; (ii) in silico analyses could be useful tools to predict different stress responses; (iii) alteration of the sEV-mediated communication of tumour cells might be a therapy-induced host response, with a potential influence on treatment efficacy.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Small extracellular vesicles convey the stress-induced adaptive responses of melanoma cells

Harmati

Gyukity-Sebestyen

Dobra

et al. 2019

Sci Rep

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“…When preventive hygiene measures (GHP), including C&D and a hygienic slaughter process, fail to prevent contamination of carcasses with pathogenic bacteria, additional control measures such as carcass disinfection, more correctly called 'decontamination' can be applied (Hawley & Kozlovac 2005;De Busser et al 2013). For beef, removal of Salmonella and STEC is a high priority.…”

Section: Decontamination Of Carcassesmentioning

confidence: 99%

Foodborne bacteria in slaughterhouses : with focus on cleaning and disinfection

Moazzami

2023

Acta Universitatis Agriculturae Sueciae

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Proper cleaning and disinfection (C&D) in slaughterhouses and meat processing premises is a prerequisite for production of safe, high-quality meat. An example of how inadequate C&D can affect human health is provided by the strong increase in number of human cases of campylobacteriosis in Sweden in 2016-2017 due to insufficiently cleaned transport crates for chicken. This thesis evaluated the efficacy of C&D procedures in slaughterhouses and adjacent meat processing premises by testing for pathogenic and hygiene indicator bacteria on equipment, facilities and carcasses. Different sampling methods used for assessing surface cleanliness were compared and slaughterhouses with associated meat processing premises in Sweden were interviewed regarding their C&D routines and monitoring activities. The results indicated that the C&D procedures used for surfaces in the slaughterhouse premises were sufficient regarding removal/inactivation of pathogenic bacteria. However, only approximately half of the surfaces were assessed as sufficiently cleaned, as the amount of hygiene indicator bacteria was high. Campylobacter jejuni, C. coli and Listeria monocytogenes were detected on food contact surfaces, indicating high risk of contamination of carcasses and meat. The same L. monocytogenes strain was found in a red meat slaughterhouse environment on three occasions 15 weeks apart, while a C. jejuni strain showed potential to spread within a poultry slaughterhouse. Extended-spectrum beta-lactamase (ESBL) Escherichia coli was not detected. New technologies such as 265 nm UV-C LED irradiation on chicken transport crates and ultrasound-steam treatment and chilling of broiler carcasses were shown to give significant bacterial reductions. However, these techniques require a certain time of action that is difficult to combine with high slaughter speed in an industrial setting. Dipslides and ATP-bioluminescence were found to be of varying reliability, but are commonly used by Swedish slaughterhouses with associated meat processing premises.

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“…Preparation includes establishing the necessary infrastructure, organizing and staging engineered decontamination processes, ensuring safe working conditions, and preventing the spread of contamination. The choice of decontamination technologies (Hawley and Kozlovac 2004) depends primarily on the BWA, matrix to be decontaminated (e.g., outdoor, semienclosed, indoor, or water system), the extent and magnitude of contamination, effects of decontamination reagents on sensitive and other high-value equipment in the site to be treated, safety and health concerns, and stakeholder issues. Additional logistical factors that determine the feasibility of a particular approach include the adequacy and availability of a decontaminant delivery system, availability of qualified personnel, and accessibility to contaminated locations.…”

Section: Decontaminationmentioning

confidence: 99%

“…Decontaminating an area or item contaminated by a BWA involves numerous issues specific to an individual location (see for example Hawley and Kozlovac 2004; OSHA anthrax etool, available at <http://www.osha.gov/SLTC/etools/anthrax/>; Canter et al 2005; and <www.epa.gov/nhsrc/dcm.htm>). Regulatory requirements will guide decontamination activities, and the efforts will almost certainly be coordinated by the EPA OSC, with input from the CDC, under CERCLA regulations.…”

Section: No Single Decontamination Technology or Strategy Is Effectivmentioning

confidence: 99%