Decontamination and Biomodification of Periodontally Affected Root Surface for Successful Regeneration

Shibli, Jamil Awad; Feres, Magda; Figueiredo, Luciene Cristina; Santos, Nídia Cristina Castro dos; Retamal‐Valdes, Belén

doi:10.1016/j.cden.2021.06.001

Cited by 4 publications

(2 citation statements)

References 53 publications

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“…Bearing this in mind, multiple therapies have been suggested in addition to mechanical disruption for the treatment of oral diseases caused by biofilms, with the aim of gaining direct control of the infectious agent [ 1 ]. In the literature, different treatments have been proposed for decontaminating teeth and implant surfaces as adjunctive therapies in treating periodontal and peri-implant diseases of bacterial origin [ 7 , 8 ]. These adjunctive therapies include the use of antibiotics and antiseptics [ 9 , 10 ], lasers [ 11 , 12 ], biological approaches such as the use of prebiotics and probiotics [ 13 , 14 ], the use of phages and Lysins [ 15 , 16 , 17 ], and dietary interventions [ 18 ], among others.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Methylene Blue Associated with Photodynamic Therapy: In Vitro Study in Multi-Species Oral Biofilm

Bueno-Silva,

Parma-Garcia,

Frigo

et al. 2024

Pathogens

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The control of infectious diseases caused by biofilms is a continuing challenge for researchers due to the complexity of their microbial structures and therapeutic implications. Photodynamic therapy as an adjunctive anti-infective treatment has been described as a possible valid approach but has not been tested in polymicrobial biofilm models. This study evaluated the effect of photodynamic therapy in vitro with methylene blue (MB) 0.01% and red LEDs (λ = 660 nm, power density ≈ 330 mW/cm2, 2 mm distance from culture) on the metabolic activity and composition of a multispecies subgingival biofilm. Test Groups LED and MB + LED showed a more significant reduction in metabolic activity than the non-LED application group (~50 and 55%, respectively). Groups LED and MB equally affected (more than 80%) the total bacterial count in biofilms. No differences were noted in the bacterial biofilm composition between the groups. In vitro LED alone or the MB + LED combination reduced the metabolic activity of bacteria in polymicrobial biofilms and the total subgingival biofilm count.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Methylene Blue Associated with Photodynamic Therapy: In Vitro Study in Multi-Species Oral Biofilm

Bueno-Silva,

Parma-Garcia,

Frigo

et al. 2024

Pathogens

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“…[ 14 15 ] Recent systematic reviews have found no substantial therapeutic advantages of decontamination or biologic modification of currently available RB agents on damaged roots, emphasizing the need for additional biologically acceptable agents to improve regeneration. [ 16 17 18 ]…”

Section: Introductionmentioning

confidence: 99%

Scanning electron microscopic analysis of 5% glycolic acid as a novel alternative to 17% ethylenediaminetetraacetic acid in root biomodification: An in vitro study

Gottumukkala,

Lakshmi,

Penmetsa

et al. 2024

Journal of Indian Society of Periodontology

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Background: Nonsurgical periodontal therapy results in the formation of a smear layer which inhibits tissue regeneration. Root biomodification (RB) using various agents has been tried for the enhancement of new attachment formation. However, no substantial therapeutic advantages of currently available root conditioning agents have been reported emphasizing the need for additional biologically acceptable agents. Glycolic acid (GA) due to its antimicrobial nature and ability of initiation and proliferation of fibroblasts may potentially modify root surface enabling regeneration. Materials and Methods: Eighty specimens from 40 single-rooted teeth were treated with 17% ethylenediaminetetraacetic acid (EDTA) and 5% GA and scanning electron microscopy analysis was done. The micrographs were examined for the evaluation of smear layer removal, total number of dentinal tubules, total number of patent dentinal tubules, mean diameter and surface area of dentinal tubules, and dentin erosion. Statistical analysis was done using unpaired t-test for intergroup comparison. Results: The efficacy of smear layer removal (P = 0.01) and dentin erosion (P = 0.042) was significantly better in the GA group. Both the groups showed no difference in dentinal tubule-related parameters. Conclusion: GA showed improved RB with greater smear layer removal and lesser dentin erosion, indicating its use as a potent alternative to the conventional EDTA root conditioning.

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Root surface biomodification in periodontal therapy: Biological rationale and clinical applications

Saleh,

Dias,

Ravida

et al. 2024

Periodontology 2000

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Regenerative periodontal therapy aims to form new cementum, periodontal ligament, and alveolar bone, all sealed by gingival tissue. The root surface acts as the wound margin during this regeneration process. Root surface biomodification (root conditioning/root decontamination), therefore, seems instrumental in promoting surface decontamination and enhancing tissue attachment by removing the smear layer, exposing collagen fibrils, and facilitating blood clot formation and stabilization. This review attempted to provide an all‐encompassing, evidence‐based assessment of the role of root surface biomodification in regenerative periodontal therapy, particularly in intrabony defects, furcation defects, and root coverage procedures. The reviewed evidence suggested that root conditioning agents, whether used independently or in conjunction with bone graft materials, biological agents, membranes, or connective tissue grafts, do not offer any clinical advantage regarding clinical attachment gain. Thus, integrating chemical methods with the mechanical root instrumentation process does not necessarily contribute to superior clinical outcomes.

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Decontamination and Biomodification of Periodontally Affected Root Surface for Successful Regeneration

Cited by 4 publications

References 53 publications

Antimicrobial Activity of Methylene Blue Associated with Photodynamic Therapy: In Vitro Study in Multi-Species Oral Biofilm

Antimicrobial Activity of Methylene Blue Associated with Photodynamic Therapy: In Vitro Study in Multi-Species Oral Biofilm

Scanning electron microscopic analysis of 5% glycolic acid as a novel alternative to 17% ethylenediaminetetraacetic acid in root biomodification: An in vitro study

Root surface biomodification in periodontal therapy: Biological rationale and clinical applications

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