Decontamination of Human Xenotransplantable Tumor with Mouse Hepatitis Virus by Implantation in Nude Rat: A Case Report.

Abstract

Abstract:A human tumor xenograft contaminated with mouse hepatitis virus (MHV) was implanted in a nude rat in order to decontaminate the tumor line. The decontamination failed in the first trial, but succeeded in the second trial. The difference between the two trials was the duration of implantation of the tumor in the nude rat, i.e., 12 days in the first and 24 days in the second trial. Duration of implantation might be a factor in the decontamination of transplantable tumors infected with MHV by passaging in the nude rat. Key words: human neoplasm, mouse hepatitis virus, xenograft Moreover, when HTX-bearing mice are infected with MHV, the HTX is easily contaminated with the virus. Therefore, the tumor line itself becomes one of the main sources for transmission of the virus into mouse colonies [6,9], and microbiological control of tumor lines including MHV is very important to assure the reliability and reproducibility of results of experiments using HTX/immune deficient animal models.We examined the microbiological status of established or introduced HTXs by using a quarantine system [14], and found that an HTX line introduced from a certain research institute was contaminated with MHV. In a previous report, Rülicke et al. [9] used nude rats as the host for xenotransplantation of tumors contaminated with MHV, and succeeded in decontamination of the tumors. To eliminate the virus contaminating the HTX, we also passaged the tumor in nude rats.

From Bench to Cageside: Risk Assessment for Rodent Pathogen Contamination of Cells and Biologics

“…There are numerous reports of contamination of tumor cell lines with lymphocytic choriomeningitis virus (LCMV; Bhatt et al 1986;Dykewicz et al 1992), mouse hepatitis virus (MHV 1 ; Takakura et al 2000), lactate dehydrogenase-elevating virus (LDHV 1 ; Chen and Plagemann 1997), mouse parvovirus (MPV 1 ; Garnick 1996), and retroviruses (Suzuki et al 1977). Both the zoonotic potential (Bowen et al 1975) and a recently reported endemic infection (Ike et al 2007) of LCMV underscore the importance of continued surveillance for this organism.…”

Mouse Hepatitis Virus

“…For example, MHV contamination of a human tumor xenograft that was maintained in nude mice (the source of infection) was eliminated by passage in nude rats for 24, but not 12 days (Takakura et al 2000). It requires selecting the minimal number of breeding founders possible, isolating them from the infected colony of mice, isolating them from each other, and building up the rederived colony in isolation.…”