Deconvolution improves the detection and quantification of spike transmission gain from spike trains

Spivak, Lidor; Levi, Amir; Sloin, Hadas E.; Someck, Shirly; Stark, Eran

doi:10.1038/s42003-022-03450-5

Cited by 12 publications

(25 citation statements)

References 79 publications

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“…b the number of putative inhibitory connections increased significantly following the washout of gabazine at DIV 14. Putative pair-wise inhibitory connections were inferred by a cross-correlogram approach and using an algorithm to infer reductions in spike transmission probability 102 . c The wiring parameters of the best performing (matching) generative model.…”

Section: Resultsmentioning

confidence: 99%

“…To further examine the extent to which washing out gabazine returned endogenous inhibitory activity as GABA A receptors are released from blockade, we also computed the spike transmission probability (STP; Supplementary Figure 13 ). This cross-correlogram based metric has been used to infer putative inhibitory functional connectivity from ongoing extracellular spiking activity and to identify neuron pairs with a reduction in spike transmission gain (STG) 102 . Indeed, we find that washout of gabazine led to a significant increase in the number of putative inhibitory connections (Wilcoxon signed rank: p=0.012; Figure 6b ).…”

Section: Resultsmentioning

confidence: 99%

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Homophilic wiring principles underpin neuronal network topologyin vitro

Akarca

Dunn

Hornauer

et al. 2022

Preprint

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Economical efficiency has been a popular explanation for how networks organize themselves within the developing nervous system. However, the precise nature of the economic negotiations governing this self- organization remain unclear. We approach this problem by combining high-density microelectrode array (HD-MEA) recordings, which allow for detailed characterization of the ongoing extracellular electrical activity of individual neurons in vitro, with a generative modeling approach capable of simulating network formation. The best fitting model uses a homophilic generative wiring principle in which neurons form connections to other neurons with similar connectivity patterns to themselves. This homophily-based mechanism for neuronal network emergence accounts for a wide range of observations that are described, but not sufficiently explained, by traditional analyses of network topology. Using rodent and human monolayer and organoid cultures, we show that homophilic generative mechanisms account for the topology of emerging cellular functional connectivity, representing an important wiring principle and determining factor of neuronal network formation in vitro.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Homophilic wiring principles underpin neuronal network topologyin vitro

Akarca

Dunn

Hornauer

et al. 2022

Preprint

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“…To this end, putative monosynaptic excitatory (PMSE) pairs were sought between all possible mPFC and DMS pairs (Fig.7A). PMSE pairs were identified based on well-established methods with minor modifications 33,34 . The criterion is based on a strongly increased likelihood of spiking of the postsynaptic pair member with minimal jitter within a narrow time window (monosynaptic delay) after the presynaptic member’s spikes (see methods for details).…”

Section: Resultsmentioning

confidence: 99%

Action-outcome based flexible behavior requires medial prefrontal cortex lead and its enhanced functional connectivity with dorsomedial striatum

Kőszeghy,

Xu,

Liu

et al. 2023

Preprint

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Cognitive flexibility plays a key role in ensuring an individual’s survival, and its deficit is a key symptom in many mental conditions and neurodegenerative diseases. The prefrontal cortex and striatum are both essential to cognitive flexibility. However, how the prefrontal cortex and striatum communicate with each other to enable flexible decision-making is not well understood. Competing theories are raised, debating on which structure among these two leads the role in detecting and representing the new circumstances for a change, giving largely opposing predictions on neural activities in the prefrontal cortex and striatum during flexible behavior.To address this question, we trained head-restrained mice to perform an action-outcome based dynamic foraging task and simultaneously recorded single-neuron activities in the medial prefrontal cortex (mPFC) and dorsomedial striatum (DMS). In this task, the animal chooses one of two actions to obtain reward. The animal is guided only by previous reward outcomes. We report that mPFC but not DMS activity stores information about prior reward history. A large fraction of both mPFC and DMS neurons’ activity represents the difference in reward probability between two alternative options, namely the perceived reward probability difference (PRPD), a key decision variable that prescribes which subsequent choice to make. We find that mPFC neural activities track the change of PRPD earlier and faster than those in the DMS, and functional connectivity between mPFC and DMS increases with reducing overall reward proportion.

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“…To determine whether a monosynaptic connection may exist between units, count cross-correlation histograms (CCHs; 0.1 ms bins) were constructed for putative pre- and postsynaptic spike train pairs as previously described 93 . Briefly, the spike transmission curve was estimated by the difference between the deconvolved CCH and the baseline, determined by hollowed median filtering of the count CCH, scaled to spikes/s.…”

Section: Methodsmentioning

confidence: 99%

Positive and biphasic extracellular waveforms correspond to return currents and axonal spikes

Someck

Levi

Sloin

et al. 2023

Preprint

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Multiple biophysical mechanisms may generate non-negative extracellular waveforms during action potentials, but the origin and prevalence of positive spikes and biphasic spikes in the intact brain are unknown. Using extracellular recordings from densely-connected cortical networks in freely-moving mice, we find that a tenth of the waveforms are non-negative. Positive phases of non-negative spikes occur in synchrony or just before wider same-unit negative spikes. Narrow positive spikes occur in isolation in the white matter. Isolated biphasic spikes are narrower than negative spikes, occurring right after spikes of verified inhibitory units. In CA1, units with dominant non-negative spikes exhibit place fields, phase precession, and phase-locking to ripples. Thus, near-somatic narrow positive extracellular potentials correspond to return currents, and isolated non-negative spikes correspond to axonal potentials. Identifying non-negative extracellular waveforms that correspond to non-somatic compartments during spikes can enhance the understanding of physiological and pathological neural mechanisms in intact animals.

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Deconvolution improves the detection and quantification of spike transmission gain from spike trains

Cited by 12 publications

References 79 publications

Homophilic wiring principles underpin neuronal network topologyin vitro

Homophilic wiring principles underpin neuronal network topologyin vitro

Action-outcome based flexible behavior requires medial prefrontal cortex lead and its enhanced functional connectivity with dorsomedial striatum

Positive and biphasic extracellular waveforms correspond to return currents and axonal spikes

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