Decorated ultrathin bismuth selenide nanosheets as targeted theranostic agents for in vivo imaging guided cancer radiation therapy

Abstract

An efficient radiotherapeutic agent is synthesized using ultrathin two-dimensional 30-nm-wide and 2-nm-thick Bi 2 Se 3 nanosheets (NSs) as a radiosensitizer. Chitosan (CS) and RGD peptide are employed to enhance the radiotherapy efficiency and biocompatibility. The Bi 2 Se 3 -CS-RGD NSs exhibit excellent targeting ability to αvβ3 integrin-overexpressing cancer cells and potent radiosensitization efficiency with high stability. Detailed in vitro experiments show that the Bi 2 Se 3 -CS-RGD NSs enhance the sensitivity of HeLa cells to X-ray-induced cell death by inhibiting TrxR activities and activating downstream reactive oxygen species-mediated signaling pathways. In vivo experiments using intravenous or intratumor injection demonstrate that the Bi 2 Se 3 -CS-RGD NSs are more efficient tumor growth inhibitors compared to bare Bi 2 Se 3 NSs. The multifunctionality of the NSs enables the use of photoacoustic imaging and magnetic resonance imaging to examine their targeting ability and therapeutic effects, respectively. In addition, the RGD-decorated Bi 2 Se 3 NSs show much better in vivo biocompatibility and can be efficiently expelled from the body after 48 h post injection. This study reveals an effective and safe theranostic agent for next-generation cancer radiotherapy.

The versatile biomedical applications of bismuth-based nanoparticles and composites: therapeutic, diagnostic, biosensing, and regenerative properties

“…Similar to this study, successful active targeting of the tumor cells was reported using LyP-1 peptide conjugated PEG-Bi 2 S 3 NPs, 210 Trastuzumab (Tam) conjugated Bi 2 S 3 @MSN core-shell NPs, 313 chitosan (CS)-RGD modified Bi 2 Se 3 nanosheets (Fig. 15c), 314 and folate conjugated perfluorohexane core-lipid shell particles carrying Bi 2 S 3 NPs in the lipid shell (Fig. 15d).…”

Selenadiazole Derivatives Inhibit Angiogenesis‐Mediated Human Breast Tumor Growth by Suppressing the VEGFR2‐Mediated ERK and AKT Signaling Pathways

“…The effects of 1 a on the expression levels of proteins associated with different signaling pathways were examined by western blotting analysis as previously described …”

“…The effects of 1a on the expression levels of proteins associated with different signaling pathways were examined by western blotting analysis as previously described. [30] Statistical Analysis Experiments were carried out at least in triplicate and repeated three times. All data were expressed as mean AE standard deviation (SD).…”

Tea regimen, a comprehensive assessment of antioxidant and antitumor activities of tea extract produced by Tie Guanyin hybridization

“…The antioxidative activities of STE and ATE were measured by ABTSc + free radical scavenging assay as previously described. 26,27 Briey, 0.1 mL ABTS reagent was mixed with 0.1 mL of sample or PBS buffer and trolox. The absorbance was measured at 734 nm and half maximal inhibitory concentration (IC 50 ) was calculated to evaluate antioxidant capacity of the STE and ATE.…”