Nitric oxide (NO)
plays a crucial and important role in cellular
physiology and also acts as a signaling molecule for cancer in humans.
However, conventional detection methods have their own limitations
in the detection of NO at low concentrations because of its high reactivity
and low lifetime. Herein, we report a strategy to fabricate Pt nanoparticle-decorated
electrochemically reduced graphene oxide (erGO)-modified glassy carbon
electrode (GCE) with efficiency to detect NO at a low concentration.
For this study, Pt@erGO/GCE was fabricated by employing two different
sequential methods [first GO reduction followed by Pt electrodeposition
(SQ-I) and Pt electrodeposition followed by GO reduction (SQ-II)].
It was interesting to note that the electrocatalytic current response
for SQ-I (184 μA) was ∼15 and ∼3 folds higher
than those of the bare GCE (11.7 μA) and SQ-II (61.5 μA).
The higher current response was mainly attributed to a higher diffusion
coefficient and electrochemically active surface area. The proposed
SQ-I electrode exhibited a considerably low LOD of 52 nM (S/N = 3)
in a linear range of 0.25–40 μM with a short response
time (0.7 s). In addition, the practical analytical applicability
of the proposed sensor was also verified.