Decoupled fluorescence Monte Carlo model for direct computation of fluorescence in turbid media

Abstract

Abstract. We present a decoupled fluorescence Monte Carlo (dfMC) model for the direct computation of the fluorescence in turbid media. By decoupling the excitation-to-emission conversion and transport process of the fluorescence from the path probability density function and associating the corresponding parameters involving the fluorescence process with the weight function, the dfMC model employs the path histories of the excitation photons and the corresponding new weight function to directly calculate the fluorescence. We verify the model's accuracy using phantom experiments and compare it with that of the perturbation fluorescence Monte Carlo model. The results indicate that the model is accurate for the direct fluorescence calculation and, thus, has great potential for application in fluorescence-based in vivo tomography. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Accurate quantification of fluorescent targets within turbid media based on a decoupled fluorescence Monte Carlo model

“…In addition, the pfMC method does not rely on the reciprocity of the source and detector and is more efficient than the afMC method when many detectors are employed to improve the quality of the reconstructed image [9]. However, the pfMC method invalidly assumes that the fluorescence photons are launched anisotropically instead of isotropically.Recently, a decoupled fluorescence Monte Carlo (dfMC) model was directly derived from the RTEs [10,11]. The decoupling transformation makes the excited fluorescence photons and their propagation equivalent to the relevant excitation photons, so the dfMC model is unbiased and thus highly accurate over a wide range of optical parameters in complex Letter…”

“…Recently, a decoupled fluorescence Monte Carlo (dfMC) model was directly derived from the RTEs [10,11]. The decoupling transformation makes the excited fluorescence photons and their propagation equivalent to the relevant excitation photons, so the dfMC model is unbiased and thus highly accurate over a wide range of optical parameters in complex heterogeneous media.…”

“…On the basis of the dfMC model [10], if an excitation photon beam q with weight w ex q p passes through fluorescence zones, the weight of the resulting fluorescence photon beam can be calculated along the path history of the excitation photon beam by the following relation:…”