Decreas of BCL-2 Expression by Ethanol Extract of Ocinum basilicum L. Leaves in Breast Cancer Cells

Abstract

Ocimum basilicum L has proven to have in vitro cytotoxic activity against breast cancer cells. Pathways that cause cell death can involve one of the proteins, which is BCL-2. This study aims to determine the decrease of BCL-2 protein expressions in breast cancer cells (T47D and MCF-7) tat are treated with the ethanol extract of Ocimum basilicum L. Ocimum basilicum L. was extracted using the maceration method with 70% ethanol solvent. The concentration of ethanol extract of Ocimum basilicum L. used to see the expression of BCL-2 protein in T47D and MCF-7 cells was 199 µg/ml and 388 µg / mL. The observation of BCL-2 protein expression is using immunocytochemical methods of T47D and MCF-7 cancer cells. The results showed that the ethanol extract of Ocimum basilicum L could reduce BCL-2 protein expression in breast cancer cells (T47D and MCF-7) at concentrations of 199 µg/ml and 388 µg/ml, respectively.

Ocimum basilicum L. (basil) presents pro-apoptotic activity in an Ehrlich’s experimental tumor murine model

“…Corroborating this study, it was previously demonstrated that the ethanolic extract of O. basilicum decreases the expression of the Bcl-2 protein in breast cancer cells (T47D and MCF-7) in vitro at concentrations of 199 and 388 µg/ mL, respectively 39 . Moreover, O. basilicum extract reverts the increase of Bcl-2 content from testicular tissue induced by acute exposure of cadmium in albino rats, suggesting that the effect of Basil extract on Bcl-2 is maintained in tissues other than breast tissue.…”