Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving

Abstract

During the postpartum period there is a high incidence of mastitis in dairy cows. The reason for this increased risk of mastitis still remains unclear. Since leukocytes in colostrum have an important role in preventing the onset of mastitis, we investigated the leukocyte populations, which express CD4, CD8, CD14, CD21 or WC1, in colostrum as well as in blood obtained from 14 Holstein cows. Eight cows developed mastitis within a week after calving and the other 6 remained healthy. The percentage of CD14+ cells in colostrum was significantly lower in mastitic cows than in healthy cows. There were no significant differences in other marker positive cells either in the colostrum or in the blood. The CD14+ cells in colostrum play an important role of defense against invading microorganisms in the mammary glands. Our results suggested that the lower percentage of CD14+ cells in colostrum might predict the incidence of mastitis in the following period.

Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

“…In the present study, the mean percentages of CD2 + and CD14 + CMC were comparable or lower than percentages found in previous studies (Park et al, 1992;Yang et al, 1997;Kiku et al, 2010). The mean percentage of CD21 + CMC was much lower than the percentage of B-lymphocytes found in bovine colostrum in other studies (Park et al, 1992;Kiku et al, 2010). Our results concerning B-lymphocytes agree with others (Taylor et al, 1994;Harp et al, 2004), as T-lymphocytes traffic selectively into bovine mammary gland secretions whereas B-lymphocytes represent a minor population as compared with peripheral blood.…”

“…Cows in parity ≥4 suffered from more profound periparturient impairment of neutrophil function than younger cows (Gilbert et al, 1993), again confirming the present results. In the present study, the mean percentages of CD2 + and CD14 + CMC were comparable or lower than percentages found in previous studies (Park et al, 1992;Yang et al, 1997;Kiku et al, 2010). The mean percentage of CD21 + CMC was much lower than the percentage of B-lymphocytes found in bovine colostrum in other studies (Park et al, 1992;Kiku et al, 2010).…”

“…In general, the composition of the lymphoid compartment in the lactating mammary gland differs from that in peripheral blood, as demonstrated in the present and other studies (Park et al, 1992;Wirt et al, 1992;Van Kampen et al, 1999). Furthermore, the percentages of the different CMC subpopulations depend on differences in the techniques used to identify them (Prescott and Breed, 1910;Concha et al, 1980;Nonnecke and Kehrli, 1985), the gating strategy (Ohtsuka et al, 2010), the definition of colostrum (Archambault et al, 1988a), and the health status of the udder (Kiku et al, 2010).…”

Sladek Z, Rysanek D: CD14 expression, apoptosis and necrosis in resident and inflammatory macrophages from virgin bovine mammary gland

“…+ proportion of about 60% in the peripheral blood of lactating dairy cows and Kiku et al (2010) who reported a rate of about 40% in cows postpartum.…”

The effect of buffalo CD14 shRNA on the gene expression of TLR4 signal pathway in buffalo monocyte/macrophages

“…The gene function of CD14 and several inflammatory cytokines has been widely explored in certain diseases that are caused by Gram-negative bacteria in humans, mice and livestock. Examples include endometritis, Brucella and mastitis [12][13][14]. Most of our understanding of the molecular regulatory mechanism of CD14 has been based on the results of mouse RAW264.7 macrophage studies [15][16][17][18].…”