Decrease in free-radical production with age in rat peritoneal macrophages

Alvarez, Eloísa; Conde, Manuel; Machado, Alberto; Sobrino, Francisco; Marı́a, Consuelo Santa

doi:10.1042/bj3120555

“…Once the pathogen has been ingested, the next step is its destruction. This set of processes is mediated by destructive granule enzymes and reactive oxygen species that are generated during the respiratory burst via activation of NADPH oxidase complexes (Alvarez et al 1995). Superoxide anion (0 2 -), produced by a membrane oxidase that uses 0 2 and NADPH as substrates (Rossi, 1986), is increased during the phagocytic process and participates in the microbicidal process.…”

Section: Discussionmentioning

confidence: 99%

Ageing modulates some aspects of the non-specific immune response of murine macrophages and lymphocytes

Ortega¹,

Garcı́a²,

Fuente³

2000

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The deterioration of the immune system with ageing, which leads to an increased morbidity and mortality from infections, appears to be related to decreases in specific lymphocyte functions. However, the alteration of non-specific immunity is a more controversial subject. Our purpose was to investigate the age-related changes of different functions of the non-specific immune response in peritoneal macrophages (adherence to tissues, mobility directed to a chemical gradient from an infectious focus or chemotaxis, phagocytosis of foreign agents and destruction of these agents by superoxide anion production) and in lymphocytes (adherence and chemotaxis) from peritoneum, axillary lymph nodes, spleen and thymus. We used young (12 weeks), adult (22 weeks), mature (48 weeks) and old (72 weeks) female BALB/c mice. The adherence capacity of macrophages and lymphocytes was greater in adult and old mice than in young animals. The chemotaxis of macrophages showed higher values in cells from young mice than in those from adult mice, increasing again in macrophages from mature and old animals. A similar behaviour was shown by phagocytosis, which reached its highest values in old animals. Anion superoxide production increased with age and again the highest values were obtained in the oldest mice. Conversely, chemotaxis of lymphocytes was higher in the adult and mature animals than in the young and old animals. We conclude that, although there is a decrease in lymphocyte chemotaxis in old animals, the non-specific immune response of macrophages instead of decreasing, may increase in aged mice with respect to the values seen in adult mice. Experimental Physiology (2000) 85.5, 519-525.

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“…This set of processes is mediated by destructive granule enzymes and reactive oxygen species that are generated during the respiratory burst via activation of NADPH oxidase complexes (Alvarez et al 1995). Superoxide anion (0 2 -), produced by a membrane oxidase that uses 0 2 and NADPH as substrates (Rossi, 1986), is increased during the phagocytic process and participates in the microbicidal process.…”

Section: Discussionmentioning

confidence: 99%

Ageing Modulates some Aspects of the Non‐Specific Immune Response of Murine Macrophages and Lymphocytes

Ortega

¹

,

Garcı́a

²

,

Fuente

³

2000

Experimental Physiology

View full text Add to dashboard Cite

The deterioration of the immune system with ageing, which leads to an increased morbidity and mortality from infections, appears to be related to decreases in specific lymphocyte functions. However, the alteration of non-specific immunity is a more controversial subject. Our purpose was to investigate the age-related changes of different functions of the non-specific immune response in peritoneal macrophages (adherence to tissues, mobility directed to a chemical gradient from an infectious focus or chemotaxis, phagocytosis of foreign agents and destruction of these agents by superoxide anion production) and in lymphocytes (adherence and chemotaxis) from peritoneum, axillary lymph nodes, spleen and thymus. We used young (12 weeks), adult (22 weeks), mature (48 weeks) and old (72 weeks) female BALB/c mice. The adherence capacity of macrophages and lymphocytes was greater in adult and old mice than in young animals. The chemotaxis of macrophages showed higher values in cells from young mice than in those from adult mice, increasing again in macrophages from mature and old animals. A similar behaviour was shown by phagocytosis, which reached its highest values in old animals. Anion superoxide production increased with age and again the highest values were obtained in the oldest mice. Conversely, chemotaxis of lymphocytes was higher in the adult and mature animals than in the young and old animals. We conclude that, although there is a decrease in lymphocyte chemotaxis in old animals, the non-specific immune response of macrophages instead of decreasing, may increase in aged mice with respect to the values seen in adult mice. Experimental Physiology (2000) 85.5, 519-525.

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“…The production of oxidative radicals also appears to decline with age, and the expression of iNOS and the production of NO are reduced in macrophages from aged rodents (10,(150)(151)(152)(153)(154)(155)(156). Similarly, the generation of reactive oxygen intermediates (oxidative burst) is lower in peritoneal macrophages from aged rodents, compared to that in peritoneal macrophages from young rodents (10,150,(155)(156)(157)(158).…”

Section: Apoptotic Cellsmentioning

confidence: 99%

Immunosenescence and macrophage functional plasticity: dysregulation of macrophage function by age‐associated microenvironmental changes

Stout

¹

,

Suttles

²

2005

Immunological Reviews

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The macrophage lineage displays extreme functional and phenotypic heterogeneity, which appears to be because, in large part, of the ability of macrophages to functionally adapt to changes in their tissue microenvironment. This functional plasticity of macrophages plays a critical role in their ability to respond to tissue damage and/or infection and to contribute to clearance of damaged tissue and invading microorganisms, to recruitment of the adaptive immune system, and to resolution of the wound and of the immune response. Evidence has accumulated that environmental influences, such as stromal function and imbalances in hormones and cytokines, contribute significantly to the dysfunction of the adaptive immune system. The innate immune system also appears to be dysfunctional in aged animals and humans. In this review, the hypothesis is presented and discussed that the observed age-associated 'dysfunction' of macrophages is the result of their functional adaptation to the age-associated changes in tissue environments. The resultant loss of orchestration of the manifold functional capabilities of macrophages would undermine the efficacy of both the innate and adaptive immune systems. The macrophages appear to maintain functional plasticity during this dysregulation, making them a prime target of cytokine therapy that could enhance both innate and adaptive immune systems.

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Decrease in free-radical production with age in rat peritoneal macrophages

Cited by 20 publications

References 43 publications

Ageing modulates some aspects of the non-specific immune response of murine macrophages and lymphocytes

Ageing modulates some aspects of the non-specific immune response of murine macrophages and lymphocytes

Ageing Modulates some Aspects of the Non‐Specific Immune Response of Murine Macrophages and Lymphocytes

Immunosenescence and macrophage functional plasticity: dysregulation of macrophage function by age‐associated microenvironmental changes

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