Decrease in the Density of IgG-Fc Receptor III (CD16) on ‘Toxic’ Neutrophils

Kabutomori, Osamu; Iwatani, Yoshinori; Koh, Taichin; Fushimi, Ryo; Amino, Nobuyuki

doi:10.1159/000204512

Cited by 11 publications

(9 citation statements)

References 7 publications

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“…In addition, no correlation was found between the density of the CD16 antigen and LAP activity in patients with PV. This lack of correlation is consistent with the finding that the density of the CD16 antigen on neutrophils was negatively correlated with the percentage of "toxic" neutrophils in patients with neutrophilia, 12 because the LAP activity of neutrophils is increased in patients with a high percentage of "toxic" neutrophils.…”

Section: Discussionsupporting

confidence: 87%

“…The red blood cells in the samples were lysed and fixed with FACS lysing solution (Becton Dickinson), and the fluorescence intensity of CD16 + cells in neutrophils was examined with a flow cytometer (FACScan, Becton Dickinson). 12 The density of CD16 antigens on neutrophils was expressed as the mean channel number of fluorescence intensity of CD16 + cells by analysis of single histograms containing 1,500 to 9,500 cells. The fluorescence compensation for the measurement was performed with the same lot of Calibrite beads (Becton Dickinson).…”

Section: Measurement Of the Cd16 Antigen Densitymentioning

confidence: 99%

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CD16 Antigen Density on Neutrophils in Chronic Myeloproliferative Disorders

et al. 1997

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Using flow cytometry, we quantitatively examined the density of the CD16 (IgG Fc receptor III) antigen on neutrophils in healthy control subjects, in patients with neutrophilia due to bacterial infection, and in patients with chronic myeloproliferative disorders (chronic myeloid leukemia [CML], polycythemia vera, or essential thrombocythemia). The density was expressed as the mean fluorescence intensity of neutrophils stained with fluorescein isothiocyanate-labeled anti-CD16 monoclonal antibody. We also determined leukocyte alkaline phosphatase activity semiquantitatively in the same population. The mean (± SD) density of the CD16 antigen on neutrophils in patients with CML (n = 13; 240.4 ± 134.8) was lower (P<.001) than in healthy control subjects (n = 25; 656.6 ± 238.0), and the density was Alkaline phosphatase activity is decreased in the neutrophils of patients with chronic myeloid leukemia (CML), but not in these cells of patients with other hematologic disorders.1 The determination of this activity is therefore useful for the differential diagnosis of CML. However, this determination is performed by a cytochemical method and is not quantitative, and measurement takes a long time. Many other biochemical and functional defects are present in the neutrophils of patients with CML.2 -3 The phagocytic capacity of neutrophils is also decreased in CML. -4 Phagocytosis is mediated mostly by neutrophils, 5 and is enhanced by the interaction of specific antibodies binding foreign bodies, such as bacteria, and IgG-Fc receptor III (CD16) on neutrophils.6 This immunophagocytosis is also decreased in CML.7 This decrease may be caused by the decrease in the density of the CD16 antigen on neu-

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Section: Discussionsupporting

confidence: 87%

Section: Measurement Of the Cd16 Antigen Densitymentioning

confidence: 99%

CD16 Antigen Density on Neutrophils in Chronic Myeloproliferative Disorders

et al. 1997

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“…The level of CD16 (Fcg receptor IIIb; CD16b is the form of CD16 expressed on human neutrophils) increases with neutrophil maturation and serves as a marker of neutrophil differentiation from myelocytes to metamyelocytes, banded neutrophils, and mature segmented neutrophils (48). Low level of neutrophil CD16 is observed in severe bacterial sepsis, infections, and inflammatory states and marks a population of "toxic" or "left-shift" neutrophils with elevated myeloperoxidase activity and compromised phagocytic and bactericidal activity (49,50). The level of CD16 is low on neutrophils induced in healthy volunteers following administration of G-CSF (51).…”

Section: Discussionmentioning

confidence: 99%

Neutrophil and Granulocytic Myeloid-Derived Suppressor Cell–Mediated T Cell Suppression Significantly Contributes to Immune Dysregulation in Common Variable Immunodeficiency Disorders

Vlková

Chovancová

Nechvátalová

et al. 2019

The Journal of Immunology

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Common variable immunodeficiency disorders (CVID) represent a group of primary immunodeficiency diseases characterized by hypogammaglobulinemia and impaired specific Ab response, resulting in recurrent infections due to dysfunctional immune response. The specific mechanisms mediating immune deficiency in CVID remain to be determined. Previous studies indicated that immune dysregulation in CVID patients is associated with chronic microbial translocation, systemic immune activation, and altered homeostasis of lymphocytic and myeloid lineages. A detailed phenotypic, functional characterization of plasma markers and immune cell populations was performed in 46 CVID patients and 44 healthy donors. CVID patients displayed significantly elevated plasma levels of a marker of neutrophil activation neutrophil gelatinase-associated lipocalin. Neutrophils from CVID patients exhibited elevated surface levels of CD11b and PD-L1 and decreased levels of CD62L, CD16, and CD80, consistent with a phenotype of activated neutrophils with suppressive properties. Neutrophils from CVID patients actively suppressed T cell activation and release of IFN-g via the production of reactive oxygen species. Furthermore, CVID was associated with an increased frequency of low-density neutrophils (LDNs)/granulocytic myeloid-derived suppressor cells. LDN/granulocytic myeloid-derived suppressor cell frequency in CVID patients correlated with reduced T cell responsiveness. Exogenous stimulation of whole blood with bacterial LPS emulated some but not all of the phenotypic changes observed on neutrophils from CVID patients and induced neutrophil population with LDN phenotype. The presented data demonstrate that neutrophils in the blood of CVID patients acquire an activated phenotype and exert potent T cell suppressive activity. Specific targeting of myeloid cellderived suppressor activity represents a novel potential therapeutic strategy for CVID.

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“…Previous studies have indicated that CD16 begins to be expressed at the metamyelocyte stage and reaches its peak at the band and segmented stages of neutrophilic development (9,10). The expression of CD16 should not be studied in the presence of infection because it is decreased or even completely absent under such circumstances (24,25). Although the percentage of CD10 + cells in the marrow tended to parallel that of segmented neutrophils, there was an observable difference between both percentages, which may be related to the limitation of morphologic identification of neutrophils.…”

Section: Discussionmentioning

confidence: 99%

Flow cytometric study of neutrophilic granulopoiesis in normal bone marrow using an expanded panel of antibodies: Correlation with morphologic assessments

Elghetany

Patel

et al. 2004

Clinical Laboratory Analysis

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Flow cytometry studies of surface markers of neutrophils have been performed mostly on peripheral blood, and for a limited spectrum of diseases. Studying maturation defects on developing neutrophils in the bone marrow (BM) may be helpful in BM diseases, such as myelodysplastic syndromes and Shwachman-Diamond syndrome. We applied an expanded panel of antibodies to examine normal maturation patterns in 26 control samples of BM together with microscopic correlation. Promyelocytes correlated well with the CD24(-) and CD11b(-) populations, and metamyelocytes correlated well with the CD16(+) population (intermediate positivity). An excellent correlation was also identified between the sum of bands and segmented neutrophils and each of the following: CD16(++) (strong positivity), CD35(+), CD87(+), and CD64(-). Although visually identified segmented neutrophils paralleled CD10 positivity, there was an appreciable difference between both methods. We conclude that neutrophilic granulocyte maturation in the BM is accompanied by a change in surface antigens that reflects certain stages of development. A successful strategy for detecting maturation defects is to include several antibodies that are known to be expressed or absent at the same stage of maturation, such as CD16, CD35, CD64, and CD87.

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Decrease in the Density of IgG-Fc Receptor III (CD16) on ‘Toxic’ Neutrophils

Cited by 11 publications

References 7 publications

CD16 Antigen Density on Neutrophils in Chronic Myeloproliferative Disorders

CD16 Antigen Density on Neutrophils in Chronic Myeloproliferative Disorders

Neutrophil and Granulocytic Myeloid-Derived Suppressor Cell–Mediated T Cell Suppression Significantly Contributes to Immune Dysregulation in Common Variable Immunodeficiency Disorders

Flow cytometric study of neutrophilic granulopoiesis in normal bone marrow using an expanded panel of antibodies: Correlation with morphologic assessments

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