Decrease of mesophyll conductance to CO2 is a possible mechanism of abscisic acid influence on photosynthesis in seedlings of pea and wheat

Sukhov, Vladimir; Gaspirovich, Vladimir; Gromova, Ekaterina; Ladeynova, Maria; Sinitsyna, Yu. V.; Berezina, Ekaterina; Akinchits, Elena; Vodeneev, Vladimir

doi:10.1134/s1990747817030096

Cited by 16 publications

(46 citation statements)

References 38 publications

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“…Seedlings of pea (Pisum sativum L., cultivar "Albumen"), wheat (Triticum aestivum L., cultivar "Zlata"), and pumpkin (Cucurbita pepo L., cultivar "Mozoleevskaya") were used as objects in our investigation, since these are important agricultural plants with photosynthetic parameters, which were investigated in our previous works [15,18,[23][24][25][26][27]. These plants were cultivated in a Binder KBW 240 plant growth chamber (BinderGmbH, Tuttlingen, Germany) at 24 • C under a 16/8 h (light/dark) photoperiod.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Light-Induced Changes in the Photochemical Reflectance Index (PRI) in Leaves of Pea, Wheat, and Pumpkin Using Pulses of Green-Yellow Measuring Light

Sukhova

Sukhov

2019

Remote Sensing

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The photochemical reflectance index (PRI) is a widely used spectral index which can show stress-induced changes in photosynthesis (e.g., increase of the nonphotochemical quenching of chlorophyll fluorescence (NPQ)). The artificial illumination of plants improves the efficiency of estimation of photosynthetic processes on the basis of PRI measurements. However, the simultaneous activity of different light sources with different locations can disturb the measurement of PRI. Using pulses of a green-yellow measuring light can potentially solve this problem. The aim of the present work was to investigate the possibility of using green-yellow light pulses for the investigation of light-induced changes in PRI in higher plants (pea, wheat, and pumpkin) and for the analysis of connection between PRI and the energy-dependent component of NPQ (NPQF). First, we showed that using green-yellow light pulses eliminated shifts of reflected light, which were connected with the application of a red actinic light. Second, analysis of light dependences of NPQF, the absolute value of PRI, and changes in PRI (the difference between the PRI under the actinic light and the initial value of PRI without this light, ΔPRI) showed that the dynamics of the increase of NPQF and the decrease of PRI and ΔPRI were similar. Changes in NPQF and ΔPRI were found to be significant. In contrast, changes in the absolute value of PRI were not significant in most of the variants of the experiments. Third, scatter plots between NPQF and ΔPRI showed similar linear correlations for investigated species; moreover, a total set of experimental points (for pea, wheat, and pumpkin) were also described by the same linear regression. Thus, our results show that (i) pulses of green-yellow measuring light can be used for measurements of PRI, and (ii) ΔPRI is a more effective indicator for the estimation of NPQ than the absolute value of PRI.

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Section: Methodsmentioning

confidence: 99%

Analysis of Light-Induced Changes in the Photochemical Reflectance Index (PRI) in Leaves of Pea, Wheat, and Pumpkin Using Pulses of Green-Yellow Measuring Light

Sukhova

Sukhov

2019

Remote Sensing

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Section: Introductionmentioning

confidence: 99%

“…There are a number of works which have investigated the influence of exogenous ABA treatment on photosynthetic processes [ 28 , 29 , 30 , 31 , 32 , 33 ]. It was shown that the treatment rapidly decreases photosynthetic CO 2 assimilation (A CO2 ) under light conditions and can increase the cyclic electron flow around photosystem I [ 29 , 30 , 31 , 32 , 33 ]. The changes are considered to be caused by a decrease in CO 2 flux into the stroma of chloroplasts because the maximal rate of CO 2 fixation [ 32 , 34 ] and the compensation value of the intercellular CO 2 concentration [ 31 , 32 , 33 ] do not seem to change.…”

Section: Introductionmentioning

confidence: 99%

“…It was shown that the treatment rapidly decreases photosynthetic CO 2 assimilation (A CO2 ) under light conditions and can increase the cyclic electron flow around photosystem I [ 29 , 30 , 31 , 32 , 33 ]. The changes are considered to be caused by a decrease in CO 2 flux into the stroma of chloroplasts because the maximal rate of CO 2 fixation [ 32 , 34 ] and the compensation value of the intercellular CO 2 concentration [ 31 , 32 , 33 ] do not seem to change. The CO 2 flux decrease can be caused by stomata closing [ 32 , 34 ]; the ABA treatment-induced decrease in the intercellular CO 2 concentration [ 29 ] and the weak ABA influence on the CO 2 assimilation in mutants, which has been shown to impair regulation of the stomatal closure by ABA [ 30 ], support this mechanism.…”

Section: Introductionmentioning

confidence: 99%

“…The CO 2 flux decrease can be caused by stomata closing [ 32 , 34 ]; the ABA treatment-induced decrease in the intercellular CO 2 concentration [ 29 ] and the weak ABA influence on the CO 2 assimilation in mutants, which has been shown to impair regulation of the stomatal closure by ABA [ 30 ], support this mechanism. However, an alternative mechanism of the ABA treatment-induced decrease in photosynthetic activity may be related to a decrease in the plasma membrane conductance for CO 2 [ 33 ]; the decrease influences the CO 2 leaf mesophyll conductance as a whole. It is important that the latter mechanism possibly plays a key role after plant spraying with moderate concentrations of exogenous ABA (e.g., 10 −6 –10 −5 M) [ 33 ], because changes in the stomata conductance are weak at these concentrations.…”

Section: Introductionmentioning

confidence: 99%

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Exogenous Abscisic Acid Can Influence Photosynthetic Processes in Peas through a Decrease in Activity of H+-ATP-ase in the Plasma Membrane

Yudina

Sukhova

Sherstneva

et al. 2020

Biology

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Abscisic acid (ABA) is an important hormone in plants that participates in their acclimation to the action of stressors. Treatment by exogenous ABA and its synthetic analogs are a potential way of controlling the tolerance of agricultural plants; however, the mechanisms of influence of the ABA treatment on photosynthetic processes require further investigations. The aim of our work was to investigate the participation of inactivation of the plasma membrane H+-ATP-ase on the influence of ABA treatment on photosynthetic processes and their regulation by electrical signals in peas. The ABA treatment of seedlings was performed by spraying them with aqueous solutions (10−5 M). The combination of a Dual-PAM-100 PAM fluorometer and GFS-3000 infrared gas analyzer was used for photosynthetic measurements; the patch clamp system on the basis of a SliceScope Pro 2000 microscope was used for measurements of electrical activity. It was shown that the ABA treatment stimulated the cyclic electron flow around photosystem I and decreased the photosynthetic CO2 assimilation, the amplitude of burning-induced electrical signals (variation potentials), and the magnitude of photosynthetic responses relating to these signals; in contrast, treatment with exogenous ABA increased the heat tolerance of photosynthesis. An investigation of the influence of ABA treatment on the metabolic component of the resting potential showed that this treatment decreased the activity of the H+-ATP-ase in the plasma membrane. Inhibitor analysis using sodium orthovanadate demonstrated that this decrease may be a mechanism of the ABA treatment-induced changes in photosynthetic processes, their heat tolerance, and regulation by electrical signals.

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Influence of the variation potential on photosynthetic flows of light energy and electrons in pea

et al. 2017

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Local damage (mainly burning, heating, and mechanical wounding) induces propagation of electrical signals, namely, variation potentials, which are important signals during the life of plants that regulate different physiological processes, including photosynthesis. It is known that the variation potential decreases the rate of CO assimilation by the Calvin-Benson cycle; however, its influence on light reactions has been poorly investigated. The aim of our work was to investigate the influence of the variation potential on the light energy flow that is absorbed, trapped and dissipated per active reaction centre in photosystem II and on the flow of electrons through the chloroplast electron transport chain. We analysed chlorophyll fluorescence in pea leaves using JIP-test and PAM-fluorometry; we also investigated delayed fluorescence. The electrical signals were registered using extracellular electrodes. We showed that the burning-induced variation potential stimulated a nonphotochemical loss of energy in photosystem II under dark conditions. It was also shown that the variation potential gradually increased the flow of light energy absorbed, trapped and dissipated by photosystem II. These changes were likely caused by an increase in the fraction of absorbed light distributed to photosystem II. In addition, the variation potential induced a transient increase in electron flow through the photosynthetic electron transport chain. Some probable mechanisms for the influence of the variation potential on the light reactions of photosynthesis (including the potential role of intracellular pH decrease) are discussed in the work.

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Decrease of mesophyll conductance to CO2 is a possible mechanism of abscisic acid influence on photosynthesis in seedlings of pea and wheat

Cited by 16 publications

References 38 publications

Analysis of Light-Induced Changes in the Photochemical Reflectance Index (PRI) in Leaves of Pea, Wheat, and Pumpkin Using Pulses of Green-Yellow Measuring Light

Analysis of Light-Induced Changes in the Photochemical Reflectance Index (PRI) in Leaves of Pea, Wheat, and Pumpkin Using Pulses of Green-Yellow Measuring Light

Exogenous Abscisic Acid Can Influence Photosynthetic Processes in Peas through a Decrease in Activity of H+-ATP-ase in the Plasma Membrane

Influence of the variation potential on photosynthetic flows of light energy and electrons in pea

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