Decreased biofilm formation by planktonic cells of Listeria monocytogenes in the presence of sodium hypochlorite

Bansal, Mohit; Dhowlaghar, Nitin; Nannapaneni, Ramakrishna; Kode, Divya; Chang, Sam K. C.; Sharma, Chander Shekhar; McDaniel, C. D.; Kiess, Aaron S.

doi:10.1016/j.fm.2020.103714

Cited by 19 publications

(7 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar phenomenon as well was observed by He et al ., 28 US decreased the membrane fluidity of E. coli O157:H7 ATCC 35150. This phenomenon may be due to the interaction between NaClO and the cytoplasmic membrane 30 . NaClO was hydrolyzed to HOCl and penetrated target cells due to the strong bactericidal effect, 31 which incapacitated bacteria through oxidation and pH changes.…”

Section: Resultsmentioning

confidence: 99%

“…This phenomenon may be due to the interaction between NaClO and the cytoplasmic membrane. 30 NaClO was hydrolyzed to HOCl and penetrated target cells due to the strong bactericidal effect, 31 which incapacitated bacteria through oxidation and pH changes. US significantly improved the outer membrane permeability, further enhanced penetration of NaClO into E. coli O157:H7 ATCC 35150 bacterial cells to increase antibacterial effect.…”

Section: Effect Of Naclo and Naclo-us Treatments On Cell Membrane Per...mentioning

confidence: 99%

See 1 more Smart Citation

Antibacterial mechanism of ultrasound combined with sodium hypochlorite and their application in pakchoi (Brassica campestris L. ssp. chinensis)

Zhang

Mujumdar

et al. 2022

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND In order to prolong the storage and inhibit microorganisms of pakchoi, the antibacterial activity and mechanism of ultrasound combined with sodium hypochlorite (NaClO‐US), the efficiency of NaClO‐US in reducing Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa as well as preserving quality of pakchoi were investigated. RESULTS Ultrasound treatment could significantly reduce the usage of NaClO solution from 800 ppm to 500 ppm. NaClO‐US decreased the counts of E. coli, S. aureus and P. aeruginosa, which disrupted the bacterial cell membrane with cytoplasmic leakage. In addition, NaClO‐US significantly increased cell membrane permeability, while cell membrane integrity decreased, the secondary structure of bacterial proteins showed several obvious changes, such as the increase of random coil content, as well as the decrease of α‐helix content. The bacterial counts, E. coli, S. aureus and P. aeruginosa population in pakchoi treated with NaClO‐US reduced by 1.89, 1.40, 1.60, 1.72 log CFU g−1, respectively compared to control sample after storage for 15 days. NaClO‐US resulted in minimum chlorophyll depletion, flavor and sensory deterioration. CONCLUSION NaClO‐US solution treatment inhibited microorganisms and prolonged storage of pakchoi. © 2022 Society of Chemical Industry.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Effect Of Naclo and Naclo-us Treatments On Cell Membrane Per...mentioning

confidence: 99%

Antibacterial mechanism of ultrasound combined with sodium hypochlorite and their application in pakchoi (Brassica campestris L. ssp. chinensis)

Zhang

Mujumdar

et al. 2022

J Sci Food Agric

View full text Add to dashboard Cite

show abstract

“…The strain was recovered on Brain Heart Infusion (BHI; Solarbio, China) medium at 37°C for 24 h, and a single colony was collected. Prior to each experiment, the strain was revived twice in 50 mL of BHI by successively culturing at 37°C for 24 h (Bansal et al, 2021). L. monocytogenes cells were collected by centrifuging at 3,000 g for 10 min at 4°C.…”

Section: Strain Suspension Preparationmentioning

confidence: 99%

Sterilising effect of high power pulse microwave on Listeria monocytogenes

Zhang¹,

Wang²,

Wu³

et al. 2022

IFRJ

View full text Add to dashboard Cite

In the present work, Listeria monocytogenes was used as the target strain to investigate the sterilising potential and mechanism of high power pulse microwave (HPPM). Results showed that the inactivation was positively correlated with the pulse frequencies and operating times. The count of Listeria monocytogenes was decreased by 5.09 log CFU/mL under 200 Hz for 9 min, which was used as the optimised condition to further explore the sterilisation mechanism. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images showed that the L. monocytogenes cells of untreated group presented intact surfaces, clear boundary, and its intracellular contents distributed uniformly in the cytoplasm. Following treatment, the cell wall surfaces began to deform in small areas, and cell membranes were severely ruptured, thus resulting in the appearance of electron transmission areas. Extracellular protein and nucleic acid contents, represented by OD260 nm and OD280 nm, increased with the increase in operating time significantly. After treatment, SDS-PAGE profiles of whole-cell proteins displayed that the protein bands became lighter or even disappeared. Na+ K+-ATPase activities and intracellular ATP content decreased by 72.97 and 79.09%, respectively. This was consistent with the cell viability of L. monocytogenes observed by confocal laser scanning microscopy. Overall, the sterilisation mechanism of HPPM on L. monocytogenes may be caused by membrane damage, intracellular component leakage, and energy metabolism hindrance.

show abstract

“…Many scientists are preoccupied with the tolerance of L. monocytogenes to biocides [ 11 – 16 ]. Other scientists are also studying the effects of various substances on biofilms of L. monocytogenes [ 17 , 18 ]. Hua et al [ 19 ] examined the efficacy of the pilot blancher for steaming to kill Listeria innocua and L. monocytogenes on surfaces, including surfaces in food processing plants in contact with food.…”

Section: Introductionmentioning

confidence: 99%