Decreased cariogenicity of a mutant of Streptococcus mutans

Stoppelaar, Joyce de; König, Klaus; Plasschaert, A.J.M.; Hoeven, J.S. van der

doi:10.1016/0003-9969(71)90186-5

Cited by 166 publications

(81 citation statements)

References 10 publications

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“…The tolerance to 40% bile, the bile-esculin reaction, and 6.5% NaCl tolerance are characteristics of S. faecalis and its varieties, S. faecium and S. durans. The majority of these strains grow at 10 C, tolerate 0.1% methylene blue milk, and yield serological group D reactions ( 16). These characteristics along with other physiological differences easily differentiate (by spectrum analysis) these organisms from S. mutans.…”

Section: Discussionmentioning

confidence: 96%

Characteristics of Streptococcus mutans Isolated from Human Dental Plaque and Blood

Facklam¹

1974

International Journal of Systematic Bacteriology

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Section: Discussionmentioning

confidence: 96%

Characteristics of Streptococcus mutans Isolated from Human Dental Plaque and Blood

Facklam¹

1974

International Journal of Systematic Bacteriology

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“…The formation of adherent glucans by cell-associated glucosyltransferase (GTF) of cariogenic streptococci has been implicated in the pathogenesis of dental caries by several investigators (De Stoppelaar et al, 1971;Hamada and Slade, 1980;Larrimore et al, 1983;Tanzer et al, 1985). However, recent studies demonstrating active, cell-free GTF in saliva and in the initial pellicle which forms on teeth have added a new dimension to the role of the enzyme in caries development Temperature (C) Fig.…”

Section: Discussionmentioning

confidence: 99%

The Activity of Glucosyltransferase Adsorbed onto Saliva-coated Hydroxyapatite

1988

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This study aimed to determine physical and kinetic properties ofglucosyltransferase (GTF) adsorbed onto hydroxyapatite (HA) surfaces.For development of a solid-phase enzyme assay, 4.0-mg samples of washed HA powder were exposed to centrifuged whole saliva (WSHA) or buffer, and subsequently exposed to a GTF solution. The activities of GTF adsorbed to H4 and that remaining in solution were measured. WSH4 was more effective in adsorbing GTF than was naked HA. Enzyme activity on the surface of WSHA was enhanced; more activity was detected on WSHA than was apparently removed from solution. A similar effect was observed when GTF was adsorbed to naked HA from a mixture with lysozyme or saliva; however, no enhancement was seen when GTF was adsorbed from a mixture with albumin. Compared with GTF in solution, adsorbed GTF displayed activity over a much wider range ofpH values. Temperature-activity profiles indicated that GTF adsorbed to surfaces had a lower temperature optimum (400C) than did soluble enzyme (45'C), and that the bound enzyme was more resistant to adverse effects of heat at elevated temperatures. The majority of glucan made by GTF adsorbed to parotid saliva-coated HA remained attached to the surface. The activity of lysozyme adsorbed to HA was reduced by adsorption of GTF to the same surface and was almost completely abolished by formation of glucans by the adsorbed GTF. These results suggest that soluble bacterial enzymes found in saliva can be incorporated into pellicle, interact with host-derived molecules on the surfaces of teeth, express enzymatic activity, and potentially influence the biological properties ofpellicle.

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“…Glucosyltransferase (GTF; EC 2.4.1.5) enzymes produced by Streptococcus mutans have been recognized as virulence factors in the pathogenesis of dental caries (13,45,54). GTF enzymes catalyze the formation of soluble and insoluble ␣-linked glucans from sucrose and contribute significantly to the dental plaque matrix polysaccharide composition (41).…”

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confidence: 99%

Effects of Compounds Found in Propolis onStreptococcus mutansGrowth and on Glucosyltransferase Activity

Koo

Rosalen²,

Cury³

et al. 2002

Antimicrob Agents Chemother

300

246

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Propolis, a resinous bee product, has been shown to inhibit the growth of oral microorganisms and the activity of bacterium-derived glucosyltransferases (GTFs). Several compounds, mainly polyphenolics, have been identified in this natural product. The present study evaluated the effects of distinct chemical groups found in propolis on the activity of GTF enzymes in solution and on the surface of saliva-coated hydroxyapatite (sHA) beads. Thirty compounds, including flavonoids, cinnamic acid derivatives, and terpenoids, were tested for the ability to inhibit GTFs B, C, and D from Streptococcus mutans and GTF from S. sanguinis (GTF Ss). Flavones and flavonols were potent inhibitors of GTF activity in solution; lesser effects were noted on insolubilized enzymes. Apigenin, a 4,5,7-trihydroxyflavone, was the most effective inhibitor of GTFs, both in solution (90.5 to 95% inhibition at a concentration of 135 g/ml) and on the surface of sHA beads (30 to 60% at 135 g/ml). Antibacterial activity was determined by using MICs, minimum bactericidal concentrations (MBCs), and time-kill studies. Flavanones and some dihydroflavonols, as well as the sesquiterpene tt-farnesol, inhibited the growth of S. mutans and S. sobrinus; tt-farnesol was the most effective antibacterial compound (MICs of 14 to 28 g/ml and MBCs of 56 to 112 g/ml). tt-Farnesol (56 to 112 g/ml) produced a 3-log-fold reduction in the bacterial population after 4 h of incubation. Cinnamic acid derivatives had negligible biological activities. Several of the compounds identified in propolis inhibit GTF activities and bacterial growth. Apigenin is a novel and potent inhibitor of GTF activity, and tt-farnesol was found to be an effective antibacterial agent.

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Decreased cariogenicity of a mutant of Streptococcus mutans

Cited by 166 publications

References 10 publications

Characteristics of Streptococcus mutans Isolated from Human Dental Plaque and Blood

Characteristics of Streptococcus mutans Isolated from Human Dental Plaque and Blood

The Activity of Glucosyltransferase Adsorbed onto Saliva-coated Hydroxyapatite

Effects of Compounds Found in Propolis onStreptococcus mutansGrowth and on Glucosyltransferase Activity

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