Decreased cellular Mg2+ concentrations in a subgroup of hypertensives – cell models for the pathogenesis of primary hypertension

Decreased membrane Mg2+ concentrations in a subgroup of hypertensives☆Membrane model for the pathogenesis of primary hypertension

“…3 For these reasons free and total intracellular Mg 2ϩ concentrations in essential hypertensives are of interest. 4 Intracellular free Mg 2ϩ concentrations in erythrocytes of essential hypertensive patients have been described by some, whereas other authors were unable to confirm these findings. [3][4] These different results in intracellular Mg 2ϩ content in essential hypertension may be due to various analytical techniques and different kinds of cell models.…”

“…4 Intracellular free Mg 2ϩ concentrations in erythrocytes of essential hypertensive patients have been described by some, whereas other authors were unable to confirm these findings. [3][4] These different results in intracellular Mg 2ϩ content in essential hypertension may be due to various analytical techniques and different kinds of cell models. Principally, either a disturbed transmembranous Mg 2ϩ transport through one of the several Mg 2ϩ transport systems, or shifts of intracellular Mg 2ϩ between the cellular compartments, come into account.…”

“…The Mg 2ϩ concentrations in plasma were measured with an atomic absorption spectroscope (Thermo Jarrell Ash Video 12, Thermo Jarrell Instrumentation Laboratory, Andover, MA), as described earlier. 4 As in previously described methods, lymphocytes were obtained from 20 mL heparinized blood by differential centrifugation. 4 -9 Then free Mg 2ϩ content was determined in lymphocytes by the fluorescent dye technique using Mag-fura-II (Molecular Probes, Eugene, OR) as an indicator for intracellular Mg 2ϩ , according to a recently described method.…”

Effects of Glutathione on Red Blood Cell Intracellular Magnesium

“…[7][8][9][10][11][12] Thus, suppressed levels of Mg i (1) directly promote cellular insulin resistance, 27 (2) are characteristic of insulin-resistant states, such as hypertension, and NIDDM, [7][8][9][10][11][12]27,28 and (3) contribute to the pressor effects of dietary salt loading in salt-sensitive hypertension. 29 Intracellular magnesium levels are lower in hypertensive than in normotensive subjects 11,12,25,30 and have been quantitatively and inversely related to systolic and diastolic blood pressure, to fasting blood glucose values, and to the hyperinsulinemic response to oral glucose loading. 11,12 Thus, we wondered to what extent the effects of glutathione on peripheral insulin action and blood pressure may be explained by interactions between glutathione and intracellular magnesium content.…”

Lead and blood pressure

“…Intracellular measurements of lead were performed in lymphocytes as a cell model. 10 Lymphocytic protein content was chosen as a reference, determined according to Bradford's method. 11 In plasma lead concentrations we found no significant difference in the two studied groups.…”