Decreased contractile response of peripheral arterioles to serotonin after CPB in patients with diabetes

Sabe, Sharif A.; Feng, Jun; Liu, Yuhong; Scrimgeour, Laura A.; Ehsan, Afshin; Sellke, Frank W.

doi:10.1016/j.surg.2018.03.007

Cited by 8 publications

(10 citation statements)

References 27 publications

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“…We have previously reported that CPB alters the responsiveness of microvasculature to various vasoactive mediators, such as phenylephrine 9 , endothelin-1 17 , and serotonin 3 . Here, we demonstrate a continuation of this pattern of diminished responsiveness following CPB.…”

Section: Discussionmentioning

confidence: 99%

Skeletal muscle microvasculature response to β-adrenergic stimuli is diminished with cardiac surgery

Ziegler

Anderson

Liu

et al. 2020

Surgery

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Background: Cardiac surgery and cardiopulmonary bypass (CPB) are associated with alterations in blood pressure in the perioperative period, which if uncontrolled, can result in end organ damage or dysfunction. Microvessels, significant contributors to blood pressure, both in the myocardium and peripheral skeletal muscle, have diminished responsiveness to major mediators of vascular tone, including thromboxane and serotonin after CPB. Responsiveness of these vessels to β-adrenergic stimulation, a major mediator of vascular tone, has not yet been studied. Here, we will investigate the role of β-adrenergic receptors in vascular tone regulation in human skeletal muscle microvessels before and after CPB.Methods: Skeletal muscle microvessels were isolated from patients undergoing cardiac surgery before and after CPB. Vessels were exposed in an ex-vivo model to the β-adrenergic agonist isoproterenol, or the direct adenylyl cyclase activator, forskolin, and the selective β-receptor antagonist ICI18.551 hydrochloride plus isoproterenol. Immunofluorescence of β-receptors and western blotting were also performed.Results: Microvessels showed diminished responsiveness to isoproterenol (10 −6 -10 −4 M) after CPB (n = 8/group, p = 0.01). Pretreatment with the selective β−2 blocker ICI18.551 (10-6 M) prevented isoproterenol-induced microvascular relaxation (p = 0.001). Forskolin-induced relaxation response was also significantly diminished post CPB (n = 4/group, p < 0.05 vs. pre CPB). No significant changes in the total protein expression of β−1, β−2 and β−3 receptors were detected by western blotting or immunofluorescence.

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Section: Discussionmentioning

confidence: 99%

Skeletal muscle microvasculature response to β-adrenergic stimuli is diminished with cardiac surgery

Ziegler

Anderson

Liu

et al. 2020

Surgery

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“…Meanwhile, certain vessels such as human umbilical arteries and skeletal muscle arterioles have been shown to dilate in response to stimulation of other serotonin receptors, such as the 5HT2B and 5HT7 receptors [48] . In addition to vasomodulatory effects, serotonin is also capable of activating phospholipase A2 (PLA2), the key initial enzyme involved in arachidonic acid metabolism and generation of inflammatory mediators like prostaglandins, lipoxygenases, and thromboxane A2 (TXA2) [49,50] . Most of the serotonin receptors belong to the GPCR family.…”

Section: Serotoninmentioning

confidence: 99%

“…Prostacyclin (PGI2) is an important vasodilator synthesized in vascular endothelium [50] . It also functions as a counterweight to the pro-thrombotic, vasoconstrictive effects of TXA2 [50] . PGI2 is synthesized from prostaglandin H2 (PGH2) with the assistance of PGI synthase.…”

Section: Pgi2 and Coxmentioning

confidence: 99%

“…PGI2 is synthesized from prostaglandin H2 (PGH2) with the assistance of PGI synthase. PGH2 is itself a product of arachidonic acid metabolism, the result of cyclooxygenase (COX) enzyme action [50] . Two COX isoforms have been identified in human cells: COX1 and COX2.…”

Section: Pgi2 and Coxmentioning

confidence: 99%

“…Studies have shown that enhanced activity of COX2 leads to a shift in favor of PGI2 over TXA2, while reduced COX2 activity (or predominant COX1 activity) produces the opposite effect [84][85][86] . Inflammatory cytokines and endothelial stress have been demonstrated to upregulate COX2 in in vitro models [50] .…”

Section: Pgi2 and Coxmentioning

confidence: 99%

See 2 more Smart Citations

Microvascular dysfunction following cardioplegic arrest and cardiopulmonary bypass

Feng¹,

Kant²,

Sellke³

2021

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Cardioplegia and cardiopulmonary bypass (CP/CPB) during cardiac surgery may cause systemic microvascular dysfunction. CP/CPB is associated with significant alterations in myogenic tone, agonist-induced vasomotor response, and endothelial function in various organs and vascular beds. These alterations can result in vessel spasm, organ malperfusion, and tissue damage. This review summarizes the current state of research in this field.

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Cardiac Surgery and Diabetes Mellitus

Robich

Sellke

2023

Contemporary Cardiology

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Decreased contractile response of peripheral arterioles to serotonin after CPB in patients with diabetes

Cited by 8 publications

References 27 publications

Skeletal muscle microvasculature response to β-adrenergic stimuli is diminished with cardiac surgery

Skeletal muscle microvasculature response to β-adrenergic stimuli is diminished with cardiac surgery

Microvascular dysfunction following cardioplegic arrest and cardiopulmonary bypass

Cardiac Surgery and Diabetes Mellitus

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