A total of seventy mature hens were used in three trials to study the relationship of serum calcium (Ca), fecal Ca and Ca intake when related to oviposition or to time of day. In all trials blood was taken at 8 a.m., 12 noon, and 4 p.m. each day and eggs were collected at two hour intervals. In trial 1, birds were fed a practical corn-soybean type diet containing 3 percent Ca ad libitum. In trial 2, birds had access (from 8 a.m. to 8 p.m. for three days) to either a 3 percent Ca diet {ad libitum) or were given a 4 percent Ca diet restricted to 75 percent that of the intake of the control birds. The restricted birds were fed at two hour intervals. Feces were collected at 8 a.m., 12 noon, 4 p.m., and 8 p.m. for three days, and at 8 a.m. and 12 noon on the fourth day. The birds were returned to a 3 percent Ca diet for three days, after which the treatments for the groups were reversed and the entire procedure repeated. In trial 3, feeding regimes for the three day period were as follows: 1) not restricted, 2) withholding feed for 16 hours on day two, and 3) withholding feed for 16 hours each day.When related to time of day, the serum Ca peaks (high or low) of the control group were the reverse of the Ca intake and fecal Ca peaks; however, when Ca intake was held constant in the restricted birds the serum Ca pattern remained unchanged, but the pattern of fecal Ca was much more pronounced. The total intake of Ca in both groups was the same. When plotted in relation to oviposition serum Ca again was highest when fecal Ca was lowest. The pattern of serum Ca was altered by dietary restriction; however, the birds adjusted to this restriction after approximately two days.
ABSTRACTExperiments using 7 day old chicks of both sexes from broiler crosses were carried out to study the effect of tryptophan supplementation to opaque-2 or normal corn 9.5% protein diets for chicks and tryptophan supplementation to opaque-2 corn sesame meal or normal corn-sesame meal 12% protein diets. A second group of studies was to determine the effects of methionine supplementation to the above diets. Each experiment lasted two weeks. The results indicated that chicks fed opaque-2 corn or opaque-2 corn-sesame meal diets do not respond to tryptophan supplementation. No growth response was observed with the normal corn-sesame meal diet. However the 9.5% normal corn diet gave significantly growth responses with the addition of this amino acid. Methionine supplementation to 9.5 or 12% protein diets from either corn did not improve performance.