Decreased in n‐3 DHA enriched triacylglycerol in small extracellular vesicles of diabetic patients with cardiac dysfunction

Abstract

PurposeDiabetic cardiomyopathy is the leading cause of death in diabetic patients, and the mechanism by which factors other than hyperglycemia contribute to the development of diabetic cardiomyopathy is unknown. Serum small extracellular vesicles (sEVs) carry bioactive proteins or nuclei, which enter into remote tissues and modulate cell functions. However, in diabetic conditions, the changes of lipids carried by sEVs has not been identified. Our study aims to explore the changes of lipids in sEVs in diabetic patients with cardiovascular disease, we hope to provide new ideas for understanding the role of lipid metabolism in the pathogenesis of diabetic cardiomyopathy.MethodsSEVs samples derived from serum of health controls (Ctrl), diabetic patients without cardiovascular diseases (DM), and diabetic patients with cardiovascular diseases (DM‐CAD) were used for lipidomics analysis. Because AC16 cells are also treated with those sEVs to confirm the entrance of cells and effects on insulin sensitivity, a lipidomics analysis on cells was also performed.Results and ConclusionsIn this study, we found that docosahexaenoic acid (DHA)‐triacylglycerides of sEVs from serums of DM‐CAD patients decreased significantly, and those sEVs could enter into AC16 cells and diminish insulin sensitivity. In addition, DHA‐triacylglycerides were also decreased in cells treated with sEVs from DM‐CAD. Therefore, DHA‐triacylglycerides carried by sEVs may mediate intercellular signaling and be associated with the incidence of diabetic cardiovascular complications.

Non-coding RNAs in lung cancer: molecular mechanisms and clinical applications

“…They participate in the regulation of various biological processes, including transcription, development, and immunity, by altering specific gene expression (18)(19)(20). Therefore, their dysregulation is closely correlated with various diseases, such as brain disease, diabetes, and cancer (21)(22)(23)(24)(25)(26)(27) NcRNA dysregulation has been reported to contribute to almost all aspects of LC development, including apoptosis, cell cycle, metastasis, and autophagy, as well as cell stemness (28-31). However, investigations of LC-related ncRNAs are still lacking.…”