Decreased intracellular histamine concentration and basophil activation in anaphylaxis

Yamaga, Satoshi; Yanase, Yuhki; Ishii, Kaori; Ohshimo, Shinichiro; Shime, Nobuaki; Hide, Michihiro

doi:10.1016/j.alit.2019.05.009

Cited by 23 publications

(18 citation statements)

References 20 publications

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“…Several reports suggested the possible involvement of basophils in systemic anaphylaxis. The number of blood basophils and the amount of intracellular histamines in basophils is decreased during anaphylaxis in humans, 155,156 Moreover, recent development of molecularly targeted therapy, such as omalizumab and dupilumab, has enabled us to gain insights into basophil pathophysiology in human allergic diseases. Further elucidation of basophil functions in mice and humans would be of great help in identification of novel therapeutic targets for the treatment of allergic disorders.…”

Section: Systemic Anaphylaxismentioning

confidence: 99%

“…Several reports suggested the possible involvement of basophils in systemic anaphylaxis. The number of blood basophils and the amount of intracellular histamines in basophils is decreased during anaphylaxis in humans, 155,156 implying the possible contribution of basophils to the development of systemic anaphylaxis. Tsujimura et al demonstrated in mice that basophils play a crucial role in systemic anaphylaxis mediated by IgG1, but not IgE through the release of platelet‐activating factor 47 .…”

Section: Roles For Basophils In the Development Of Allergic Disordersmentioning

confidence: 99%

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Basophils and their effector molecules in allergic disorders

Miyake

Shibata

Yoshikawa

et al. 2020

Allergy

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Basophils are the least common granulocytes which possess basophilic granules in their cytoplasm. Although they were first documented by Paul Ehrlich more than 140 years ago, their functional significance had long been an enigma, partly because of their rarity and the lack of analytical tools for basophils. In addition, basophils had been erroneously regarded as "blood-circulating mast cells," due to their similarities to tissue-resident mast cells, such as the expression of high-affinity IgE receptor (FcεRI) on their cell surface and the release of histamine upon their activation. Nevertheless, basophils and mast cells differ in a number of aspects. Basophils circulate in the peripheral blood under homeostatic conditions while mast cells typically reside in peripheral tissues and are hardly detected in the peripheral blood. Transcriptomic analyses revealed that basophils display distinct transcriptomic profiles from mast cells in both mice and humans. [1][2][3] Recent development of analytical tools for basophils has enabled us to identify nonredundant functions of basophils in multiple immune reactions, including allergic inflammation, and protective immunity against parasites. [4][5][6][7][8] In addition, the involvement of basophils is also demonstrated in several nonallergic disorders, such as lupus nephritis, 9-11 chronic obstructive pulmonary disease, 12 allograft rejection, 13,14 and carcinogenesis. 15,16 In this review, we summarize recent advances in our understanding of basophil biology, especially focusing on the roles of basophils in the context of allergic inflammation (Boxes 1 and 2). | ONTOG ENY OF BA SOPHIL SAlthough it is widely accepted that basophils differentiate from hematopoietic stem cells (HSCs), 17,18 the pathway of their differentiation

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Section: Systemic Anaphylaxismentioning

confidence: 99%

“…Several reports suggested the possible involvement of basophils in systemic anaphylaxis. The number of blood basophils and the amount of intracellular histamines in basophils is decreased during anaphylaxis in humans, 155,156 implying the possible contribution of basophils to the development of systemic anaphylaxis. Tsujimura et al demonstrated in mice that basophils play a crucial role in systemic anaphylaxis mediated by IgG1, but not IgE through the release of platelet‐activating factor 47 .…”

Section: Roles For Basophils In the Development Of Allergic Disordersmentioning

confidence: 99%

Basophils and their effector molecules in allergic disorders

Miyake

Shibata

Yoshikawa

et al. 2020

Allergy

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“…Снижение количества базофилов в крови (базопения) наблюдается реже -при аутоиммунной крапивнице, в остром периоде при анафилаксии и в начале проведения аллергенспецифической иммунотерапии [35,55,85].…”

Section: базофилы -клетки-эффекторы аллергического воспаленияunclassified

“…На результат теста активации базофилов влияет острота и тяжесть состояния пациента. При тяжелых острых аллергических реакциях интерпретация теста иногда затруднена, потому что может наблюдаться значительное снижение количества базофилов в периферической крови, как показано для острого периода анафилаксии [85]. Выраженная базопения может привести к статистически недостоверному результату теста, потому что для корректной интерпретации, согласно инструкциям к наборам, требуется оценить не менее 500 базофилов в каждой пробе.…”

Section: активация базофилов при аллергииunclassified

Basophil activation: theoretical aspects and use in the diagnosis of allergic diseases

Бычкова¹

2021

Med. immunol.

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Diagnostics of allergic diseases is a difficult issue, which requires distinct solutions, since this disorder is very common among the population. The overview focuses on complex diagnostics, including various methods that are most in demand at the present stage. The allergy diagnostics primarily include taking anamnesis, physical examination, instrumental and functional tests. Less often, the provocative tests are used, due to risk of severe adverse reactions. At the present stage, the role of laboratory diagnostics of allergies is growing, since, firstly, there is an increase in difficult-to-diagnose cases that require involvement of the entire medical armamentarium, and, secondly, the sensitivity and specificity of laboratory tests are improving. Among laboratory methods, the most significant are the assessment of the level of specific IgE, and the relatively new basophile activation test. The latter test is the main focus of the present review. It is functional and combines the advantages of provocative tests, during which conditions are created for the interaction of a potential allergen and effector cells of allergic inflammation, keeping safety for the patient. The data on the life cycle of basophils, their expression of membrane receptors, the content of granules, and ability to produce additional inflammatory mediators by the cells are presented. Participation of these cells in pathogenesis of allergic inflammation is being considered. Various mechanisms of basophil activation are discussed, both IgE-mediated and IgE-independent, which are similar in vivo and in vitro. Theoretical aspects of using the in vitro basophil activation test to estimate the hypersensitivity to a wide range of allergens are discussed. High sensitivity and specificity of the test for diagnosing allergies to food, household, pollen, insect and drug allergens are presented. Specific features of the basophil activation test related to the preanalytical, analytical and postanalytical stages of the study are highlighted. The factors influencing evaluation of this method are known. For example, difficulties in interpreting the test may arise while taking glucocorticosteroid hormones, in acute period of inflammation, with severe edema. The possibility of using this test to assess effectiveness of allergen-specific and anti-IgE therapy is being considered. A comparison of the basophil activation test, measurement of specific IgE and skin tests by various parameters related to performance and interpretation of results is carried out. Comprehensive diagnostics of allergic diseases, including usage of pathogenetically determined laboratory methods, will contribute to adequate treatment and, as a result, improve the health of the population.

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“…Protocols for the measurement of histamine as a major mediator released in allergic reactions were already established in the 1960s. Once released in the peripheral blood, histamine is immediately degraded by histamine methyltransferases resulting in a plasma half-life of only a few minutes, making its analysis quite challenging 13 . Aside from its instability, the monitoring of histamine was shown to have a low specificity and sensitivity for drug allergies as well as commercial food proteins and wasp venoms 12 .…”

Section: Introductionmentioning

confidence: 99%

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Wenger

Bethanis

Johnson

et al. 2021

JoVE

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Mediator release assays analyze in vitro immunoglobulin E (IgE)-mediated degranulation and secretion of mediators by effector cells, such as mast cells and basophils, upon stimulation with serial dilutions of putative allergens. Therefore, these assays represent an essential tool that mimics the in vivo degranulation process, which occurs upon allergen exposure in sensitized patients or in skin prick tests.Additionally, these assays are usually employed to investigate the allergenic potential of proteins and the reactivity of patients' sera's reactivity. Herein, we describe a simple 2-day protocol using an immortalized rat basophil leukemia cell line transfected and humanized with the human high-affinity IgE plasma-membrane receptor (FcεRI). This variant of the mediator release assay is a robust, sensitive, and reproducible in vitro cell-based system without the need to immobilize the antigen to solid matrices. The protocol consists of the following steps: (1) complement inactivation of human sera,(2) harvesting, seeding, and passive sensitization of the cells, (3) stimulation with antigen to cause mediator release, and (4) measuring of β-hexosaminidase activity as a surrogate for the released inflammatory mediators, such as histamine. The assay represents a useful tool to assess the capacity of the allergen-IgE cross-linking to trigger cell degranulation and can be implemented to standardize allergen extracts, to compare patients' reactivity to minor or major allergens and to allergenic extracts (pollen, cat dander, etc.), to investigate the potency of allergen homologs, isoforms, and fold-variants (e.g., hypoallergenicity), as well as the effects of ligands on the allergenic activity. A more recent application includes the use of the assay to monitor the treatment efficacy in the course of allergen immunotherapy.

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Decreased intracellular histamine concentration and basophil activation in anaphylaxis

Cited by 23 publications

References 20 publications

Basophils and their effector molecules in allergic disorders

Basophils and their effector molecules in allergic disorders

Basophil activation: theoretical aspects and use in the diagnosis of allergic diseases

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

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