Decreased level of phosphatidylcholine (16:0/20:4) in multiple myeloma cells compared to plasma cells: a single-cell MALDI–IMS approach

Abstract

Lipid metabolic changes under diseased conditions, particularly in solid tumors, are attracting increased attention. However, in non-solid tumors, including most hematopoietic tumors, lipid analyses are scarce. Multiple myeloma (MM) is a plasma cell disorder arising from bone marrow, and the lipid status of MM cells has not been reported yet. In this study, we analyzed flow cytometry-sorted single MM cells and normal plasma cells (NPCs) using matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), a two-dimensional label-free mass spectrometry technique for biomolecular analysis, to obtain specific lipid information. We isolated 1.31-5.77% of MM cells and 0.03-0.24% of NPCs using fluorescence-activated cell sorting (FACS). Analysis of purified cells using MALDI-IMS at the single-cell level revealed that the peak intensity and ion signals of phosphatidylcholine [PC (16:0/20:4) + H](+) at m/z 782.5 were significantly decreased in MM cells compared to NPCs. By examining particular cell populations rather than cell mixtures, our method can become a suitable tool for the analysis of rare cell populations at the single-cell level and advance the understanding of MM progression.

Exploring the Fundamental Structures of Life: Non‐Targeted, Chemical Analysis of Single Cells and Subcellular Structures

“…[85] Each MALDI-MS measurement strikes ab alance between extraction efficiencya nd analyte diffusion, which is especially problematic for tissue imaging. [83] Thec hoices for MALDI matrices and their application continue to expand as novel compounds are found to be suitable matrices for specific analyses or classes of analytes.M ALDI-MS provides broad chemical coverage of analytes,including proteins, [86] peptides, [87] lipids, [88] and small metabolites. [89] Af requently cited limitation is isobaric interference from the MALDI matrix, which complicates metabolite detection without high-resolution mass analyzers.…”

Erforschung der fundamentalen Strukturen des Lebens: Nicht zielgerichtete chemische Analyse von Einzelzellen und subzellulären Strukturen

“…[85] Jede MALDI-MS-Messung stellt ein Gleichgewicht her zwischen Extraktionseffizienz und Analytdiffusion, was insbesondere fürd ie Gewebebildgebung problematisch ist. MALDI-MS bietet einen breiten chemischen Bereich von Analyten, einschließlich Proteinen, [86] Peptiden, [87] Lipiden [88] und kleinen Metaboliten. MALDI-MS bietet einen breiten chemischen Bereich von Analyten, einschließlich Proteinen, [86] Peptiden, [87] Lipiden [88] und kleinen Metaboliten.…”

“…[83] Die Auswahl an MALDI-Matrizen und deren Anwendungen werden sich vergrçßern, sobald sich neue Verbindungen als geeignete Matrizen fürs pezifische Analysen oder Klassen von Analyten herausstellen. MALDI-MS bietet einen breiten chemischen Bereich von Analyten, einschließlich Proteinen, [86] Peptiden, [87] Lipiden [88] und kleinen Metaboliten. [89] Eine häufig genannte Einschränkung ist die isobare Beeinflussung durch die MALDI-Matrix, die die Metabolitenbestimmung ohne hochauflçsende Massenanalysatoren erschwert.…”

Surface‐printed microdot array chips coupled with matrix‐assisted laser desorption/ionization mass spectrometry for high‐throughput single‐cell patterning and phospholipid analysis

“…Especially, its ability to obtain thousands of spectra from tissues or other samples by MALDI‐MSI makes it an optimal technology for high‐throughput single‐cell analysis. Some researchers have reported the direct analysis of cellular components at thre single‐cell level using MALDI‐MS, such as single myeloma cells and single‐yeast cells . However, the disorderly scattered single cells made it difficult to collect effective and exact information by MALDI‐MSI, and manually selected cells for MALDI‐MS turned out to be low throughput.…”