Decreased levels of IL-1.ALPHA. and .BETA. in psoriatic lesional skin.

Abstract

mediates a wide range of biological activities, is thought to play an important role in many inflammatory and immunologic diseases. Normal human epidermal keratinocytes constitutively produce IL-1. Based on our previous data indicating decreased IL-1 activity in psoriatic scale extracts, in the present study, we measured immunoreactive IL-1 a and ,3 levels in the suction blister fluids as well as in the psoriatic scale extracts using enzyme immunoassay for IL-la and /3. The results showed that although similarly low levels of IL-la were detectable in the suction blister fluid from normal and psoriatic Lesional skin, and that no IL-1f was found in most of the blister fluids, indicating that IL-i a is major IL-1 species produced by human skin. As compared to those in the blister fluids, IL-la levels in the horny tissue extracts were found to be much higher, and they were significantly higher in the orthokeratotic stratum corneum extracts than in the psoriatic scale extracts. However, gel filtration of the orthokeratotic horny tissue extracts demonstrated that constituents for immunoreactive IL-la and Q were quite variable depending upon the source of the horny tissues. The present study has confirmed that IL-1 levels in the psoriatic scale extracts are decreased when compared with those in the orthokeratotic horny tissue possibly due to an increased epidermal proliferation activity associated with its high turnover rate. The role of IL-1 psoriatic lesions remains unknown. psoriasis ; suction blister ; horny tissue ; interleukin 1; ETA Interleukin 1 (IL-1), which mediates a wide range of biological reactions, is thought to play an important role in many inflammatory and immunologic diseases ). On the basis of isoelectric points, two major forms of IL-1 have been identified : IL-la (pI 5.0) and /3 (7.0) (Auron et al. 1984 ;March et al. 1985 ;Gubler et al. 1986). Biological analyses of purified native and recombinant human IL-1a and /3 have shown that these proteins possess very similar, if not identical, bioactivities in a broad variety of bioassays (Wood et al.

Regulatory Effects of Antipsoriatic Agents on Interleukin-1 α Production by Human Keratinocytes Stimulated with Gamma Interferon in vitro

“…However, CsA had a relatively weaker effect on ID 1 a production by stimulated HSC-1 cells. [10,[19][20][21], In addition, IFN-y produced by infiltrated T cells was detected in lesional sites [1][2][3]. To determine whether the dysrégula tion of IL-1 could be related to IFN-y, NHKs and HSC-1 cells were cultivated with rIFN-y and IL-1 levels were measured by ELISA.…”

Role of emollients in the prevention of skin diseases in young children

“…Роговой слой осуществляет функции регуляции врожденного и адаптивного иммунитета: представляет собой богатый резервуар цитокинов, включая IL8, IL6, IL-10, TNFa и др., антимикробных пептидов и метаболитов комменсальной флоры. В исследованиях доказано, что при проникновении фрагментов рогового слоя в дерму (при экскориациях, ксерозе кожи, разрыве эпидермальных кист и комедонов) развиваются воспалительные реакции [31,32]. Кератиноциты и другие резидентные клетки эпидермиса продуцируют цитокины, которые отвечают за контроль клеточной коммуникации.…”

Minimally-invasive Sampling of Interleukin-1α and Interleukin-1 Receptor Antagonist from the Skin: A Systematic Review of In vivo Studies in Humans

“…The advantage offered by the assessment of the local inflammatory profile in the suction blister fluid (54,55) was counterbalanced by the suggestion that the local trauma caused by the suction process might induce cytokines release and formation, thereby influencing the measured amounts (46)(47)(48)50). Other reported issues included the cumbersomeness of the technique (56), the possibility of pain during the procedure (57) and the possibility of blood contamination of the blister (52).…”