Decreased malondialdehyde levels in fish (Astyanax altiparanae) exposed to diesel: Evidence of metabolism by aldehyde dehydrogenase in the liver and excretion in water

Garcia, Danielly; Lima, Daína; Silva, Danilo Grünig Humberto da; Almeida, Eduardo Alves de

doi:10.1016/j.ecoenv.2019.110107

Cited by 56 publications

(17 citation statements)

References 64 publications

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“…Although other studies have shown that AK reduces the activities of SOD and CAT enzymes in kidney tissue, we could not nd any signi cant changes in levels of antioxidant enzymes in our study protocol (Abdel-Daim et al 2019). In accordance with our result, there are also studies showing that the levels of antioxidant enzymes such as SOD and CAT may not change due to increased oxidation and antioxidant use (Ohta and Nishida 2003;Yılmaz et al 2018) It has also been shown that Cu (II)-aminoglycoside complexes are formed by holding the copper ions of aminoglycosides in another study (Szczepanik et al 2004 (Garcia et al 2020;Lima et al 2019;Ubani-Rex et al 2017). In addition, not all lipid peroxidation process produces MDA and MDA is produced by reactions other than lipid peroxidation (Jenkins 2000).…”

Section: Discussionsupporting

confidence: 91%

“…However, there was no signi cant increase in MDA level in the AK-induced nephrotoxicity group when compare to the control group in our study. In contrast, some studies have shown that there is no change or may decrease in MDA level in oxidant exposure (Garcia et al 2020;Lima et al 2019;Ubani-Rex et al 2017). In addition, not all lipid peroxidation process produces MDA and MDA is produced by reactions other than lipid peroxidation (Jenkins 2000).…”

Section: Discussionmentioning

confidence: 96%

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Carvacrol attenuates amikacin-induced nephrotoxicity in the rat

Dost

Gunata

Özhan

et al. 2021

Preprint

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Amikacin (AK) is frequently used in the treatment of gram-negative and some gram-positive infections. However, its use is limited due to nephrotoxicity due to the increase in reactive oxygen radicals. The aim of this study was to investigate the role of carvacrol (CAR) against AK-induced nephrotoxicity in rats. Thirty-two Sprague Dawley rats were randomly divided into four groups as control (Vehicle), AK (400 mg/kg), CAR + AK (80 mg/kg CAR + 400 mg/kg AK), and AK + CAR (400 mg/kg AK + 80 mg/kg CAR) groups. AK and CAR were administered via intramuscular and per-oral for 7 days, respectively. Blood and kidney tissue samples were taken at the end of the experiment. Renal function and histopathological changes were compared, and the relevant parameters of oxidative stress and inflammation were detected. Histopathological findings (necrotic changes and dilatation and inflammatory cell infiltration) significantly increased in the AK group compared to the control group. Also, the rats in the AK group lost weight significantly. It was found that CAR treatment before and after AK significantly improved nephrotoxicity histopathologically (p < 0.05). However, this improvement was not detected biochemically. These results show that CAR treatment before and after AK improves nephrotoxicity in the histopathological level.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 96%

Carvacrol attenuates amikacin-induced nephrotoxicity in the rat

Dost

Gunata

Özhan

et al. 2021

Preprint

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“…In this research, passive immunization anti-ExbB serum was shown to decrease the expression of the inflammation-related genes IL-6, IL-8, IL-1β, and TNF-α after C. auratus challenged with P. fluorescens, whereas that of the passive cross-immunization (challenging A. hydrophila) was not obvious. In addition, the antioxidant level is one of the important indicators for fish health, and some studies have used antioxidant markers (MDA, SOD, GSH-PX, and CAT) to reveal the effect of drug, forage, and pollutants to fish (Dawood et al 2020;Garcia et al 2020;Abd El-Kader et al 2021). This research found that passive immunization and passive cross-immunization of anti-ExbB serum were unable to inhibit the expression of antioxidant factors (MDA, SOD, GSH-Px, and CAT) induced by bacteria.…”

Section: Discussionmentioning

confidence: 99%

Immune responses and protective efficacy of outer membrane protein ExbB of Pseudomonas fluorescens against Aeromonas hydrophila and Pseudomonas fluorescens affecting Carassius auratus

Sun

Jian

et al. 2021

Aquacult Int

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The outer membrane protein (ExbB) of Pseudomonas fluorescens present in the outermost layer may possess immune functions and potential applications in subunit vaccines. ExbB bioinformatic analysis elucidated that the relationship among Pseudomonas species was closer than others, and anti-ExbB serum might provide cross-protective ability to resist Pseudomonas bacterial infection in animals. ExbB was obtained via molecular cloning, prokaryotic expression, and purification. The optimal expression conditions were a strain OD 600 value of 1.0 and IPTG concentration of 0.3 mmol/L, inducing time of 3 h, and inducing temperature of 32℃. Mice immunized to ExbB could significantly increase (p < 0.05) the spleen index and leukocyte phagocytosis parameters such as phagocytic percentage (PP) and phagocytic index (PI). The specific antiserum titer (1:12,800) was obtained, which had an in vitro immune recognition effect on both P. fluorescens and Aeromonas hydrophila. Passive immunization results for anti-ExbB mouse serum to Carassius auratus showed that ExbB serum could significantly enhance PP (p < 0.05) and PI (p < 0.05). Moreover, the passive protective rate of anti-ExbB serum against P. fluorescens infection was 54% (p < 0.05), and the passive cross-protective rate against A. hydrophila was 38.4% (p < 0.05). Furthermore, the expression of immune-related genes such as IL-6, IL-8, IL-1β, and TNF-α was significantly decreased (p < 0.05) in kidneys and gills after challenging with P. fluorescens, while the downward trend was not obvious after challenging with A. hydrophila. The trend changes of antioxidant-related factors such as malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were not obvious after bacterial challenge. Interestingly, there was a significant reduction in the histopathological lesions in the kidney, spleen, and intestine of C. auratus challenged by P. fluorescens and A. hydrophila. Altogether, the results suggest that ExbB has an immune function in passive protection against P. fluorescens and passive cross-protection against A. hydrophila and can boost resistance to infection against the most dangerous bacterial pathogens infecting freshwater fish.

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“…In fish, increased lipid deposition could increase the degree of lipid peroxidation and increased MDA concentrations are commonly considered an indicator of lipid peroxidation caused by oxidative stress (Garcia et al, 2020; Xie et al, 2021). The anti‐oxidative enzymes (AOEs) play important roles in protecting fish from stress (Mu et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

High dietary lipid level decreases the immunity and disease resistance of abalone Haliotis discus hannai and affects the perilipin‐2/TLR4, JNK and Keap1/Nrf2 pathways

et al. 2021

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A 100‐day feeding trial was conducted to investigate the influences of dietary lipid levels on the immunity and mechanism in abalone Haliotis discus hannai Ino. Abalones (initial weight: 10.98 ± 0.05 g) were fed with graded levels of dietary lipid, which were 15.73, 23.41, 31.72, 38.25, 46.35, 55.63, 61.70 and 67.19 g/kg. Results showed that compared with the treatment with 38.25 g/kg of dietary lipid, 67.19 g/kg of dietary lipid increased the cumulative mortality of abalone after a challenge with Vibrio parahaemolyticus. Meanwhile, 67.19 g/kg of dietary lipid decreased the activities of lysozyme (LZ) and acid phosphatase (ACP) in serum, and the mRNA levels of β‐defensin and mytimacin 6 in hepatopancreas of abalone. The mRNA levels of tumour necrosis factor α (tnfα), activator protein 1 (ap‐1), nuclear factor κB (nf‐κb), interleukin 1 receptor‐associated kinase‐4 (irak4), myeloid differentiation primary response protein MyD88 (myd88), toll‐like receptor 2 (tlr2), tlr4 and perilipin‐2, and the protein level of interleukin‐1β (IL‐1β), TNFα, NF‐κBp65, AP‐1, MyD88 and Perilipin‐2 in hepatopancreas were up‐regulated by 67.19 g/kg of dietary lipid. However, the mRNA levels of arginase and inhibitor of κBα (iκbα) were down‐regulated. Besides, 67.19 g/kg of dietary lipid up‐regulated the mRNA levels of executor apoptosis‐related cysteine peptidase 3 (caspase‐3), caspase‐7 and B‐cell lymphoma protein‐2‐associated X protein (bax), and the protein levels of caspase‐3 and c‐Jun N‐terminal kinases (JNK) in hepatopancreas. Moreover, high dietary lipid level down‐regulated the mRNA levels of copper/zinc superoxide dismutase (cuznsod), manganese superoxide dismutase (mnsod), catalase (cat), glutathione peroxidase (gpx), glutathione‐S‐transferase (gst) and NF‐E2‐related factor 2 (nrf2), while up‐regulated the mRNA and protein levels of Kelch‐like ECH‐associated protein 1 (Keap1) in hepatopancreas. It was concluded that a high level (67.19 g/kg) of dietary lipid decreased the immunity of abalone, which might be related to the aggravated inflammation and apoptosis, and decreased anti‐oxidative capacity under Perilipin‐2/TLRs/MyD88/IRAK4/(NF‐κB or AP‐1), JNK/Bcl‐2/Bax and Keap1/Nrf2 signalling.

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Decreased malondialdehyde levels in fish (Astyanax altiparanae) exposed to diesel: Evidence of metabolism by aldehyde dehydrogenase in the liver and excretion in water

Cited by 56 publications

References 64 publications

Carvacrol attenuates amikacin-induced nephrotoxicity in the rat

Carvacrol attenuates amikacin-induced nephrotoxicity in the rat

Immune responses and protective efficacy of outer membrane protein ExbB of Pseudomonas fluorescens against Aeromonas hydrophila and Pseudomonas fluorescens affecting Carassius auratus

High dietary lipid level decreases the immunity and disease resistance of abalone Haliotis discus hannai and affects the perilipin‐2/TLR4, JNK and Keap1/Nrf2 pathways

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