Decreased Nitric Oxide Production in Patients with Behçet’s Disease

Örem, Asım; Vanizor, Birgül; Çimşit, Gülseren; Kiran, Emine; Değer, Orhan; Malkoç, Mehtap

doi:10.1159/000018061

Cited by 62 publications

(58 citation statements)

References 13 publications

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“…Serum NO levels were found to be either significantly elevated 33 or decreased 34 in BD patients compared with control subjects. The major metabolic pathway for endogenously formed NO in healthy human subjects is uptake into the red blood cells and conversion to NO 3 − and methemoglobin 35 .…”

Section: Discussionmentioning

confidence: 93%

Oxidases and oxygenases in regulation of neutrophil redox pathways in Behçet’s disease patients

Melikoğlu

Kaçar

Çört

et al. 2011

Journal of Enzyme Inhibition and Medicinal Chemistry

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Section: Discussionmentioning

confidence: 93%

Oxidases and oxygenases in regulation of neutrophil redox pathways in Behçet’s disease patients

Melikoğlu

Kaçar

Çört

et al. 2011

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…Acute inflammatory indicators including α 1 -antitrypsin, α 2 -macroglobulin and ESR were reported to be increased in the active stage of BD [14]. In the present study, mean plasma concentrations for all biochemical acute-phase parameters including α 1 -antitrypsin and α 2 -macroglobulin, neutrophil count and the ESR were found to be significantly higher (for each, p < 0.01) among the patients with active BD when compared with inactive disease and the control subjects (table 1).…”

Section: Resultsmentioning

confidence: 99%

Increased Plasma Adrenomedullin Levels in Patients with Behçet’s Disease

Evereklioğlu

Yürekli²,

et al. 2000

Dermatology

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Background: Behçet’s disease (BD) is a chronic systemic inflammatory disorder affecting multiple organs with a generalized vasculitis of arteries and veins. Endothelial dysfunction is one of the prominent features of BD. Adrenomedullin (AM) is a peptide produced not only in normal adrenal medulla but also in the vascular smooth muscle cells and endothelial cells, and its role in the course of BD has not been previously described. Objective: To detect changes of plasma AM concentrations in patients with BD compared with age- and sex-matched healthy subjects by using high-performance liquid chromatography (HPCL). We also investigated if disease activity or the duration of BD correlates with AM levels. Methods: Forty-two consecutive patients with BD (38.5 ± 11.1 years, 19 male and 23 female) and 20 healthy age- and sex-matched control subjects (39.5 ± 10.9 years, 8 male and 12 female) were included in this study. We measured plasma AM levels by HPCL, and acute-phase reactants including α₁-antitrypsin and α₂-macroglobulin, neutrophil count and the erythrocyte sedimentation rate. Results: Mean ± SD plasma AM levels in patients with BD (73.22 ± 25.55 pmol/l) were significantly higher (p < 0.001) than in healthy control volunteers (21.35 ± 12.37 pmol/l). Patients with active BD had similar plasma AM concentrations (79.32 ± 21.89 pmol/l) with patients with inactive disease (67.44 ± 29.92 pmol/l). On the other hand, patients with longer duration of the disease (mean duration, 13.9 ± 3.8 years) had significantly higher plasma AM levels (83.99 ± 19.71 pmol/l; p = 0.005) than patients (62.45 ± 26.57 pmol/l) with shorter duration of the disease (mean duration, 5.5 ± 2.3 years). All acute-phase reaction parameters were found to be significantly increased in the active disease. Conclusion: Considering its endothelial cell implications, AM may be involved in reparatory vessel endothelium mechanisms, especially in the chronic disease.

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“…In a previous study, we reported that the levels of plasma NO metabolites in patients with active Behçet's disease were lower than normal [8]. This finding may not necessarily imply functional impairment of vascular endothelium because the source of NO metabolites in the circulation may vary.…”

Section: Discussionmentioning

confidence: 84%

“…In a previous study, we showed decreased levels of plasma NO end products, nitrite and nitrate in patients with Behçet's disease [8]. As mentioned above, the source of measured plasma NO products does not represent endothelial-derived NO synthesis.…”

Section: Introductionmentioning

confidence: 83%

Effect of Plasma from Patients with Behçet’s Disease on the Production of Nitric Oxide in Cultured Human Umbilical Vein Endothelial Cells

Örem¹,

Ertürk

Çimşit

et al. 2003

Med Princ Pract

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Objective:To determine the effect of plasma from patients with Behçet's disease (BD) on the production of nitric oxide (NO) in cultured human umbilical vein endothelial cells (HUVECs). Subjects and Methods: NO levels were measured in cell culture media after 24-hour incubation of the cells with plasma obtained from 22 BD patients and 16 age/sex-matched healthy control subjects. After treatment of the patients with colchicine and/ or nonsteroidal anti-inflammatory drugs, 12 of the patients were considered to be in the inactive phase of the disease. Levels of NO production were also measured in these 12 patients. In addition to the in vitro experiments, erythrocyte sedimentation rate, · 1 -antitrypsin, · 2 -macroglobulin and neutrophil counts were measured in the patients and controls. Results: Levels of NO in active state BD patients (15.9 Ìmol/10 6 cells) were significantly lower than values obtained from both patients in the inactive period (19.2 Ìmol/10 6 cells) and the control group (19.7 Ìmol/10 6 cells). No significant differences were observed in induced NO products between the patients in the inactive stage and control subjects. Conclusion: Plasma from BD patients decreased the level of NO production in the HUVECs, and therefore may cause dysfunction in the endothelial NO synthase activity.

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Decreased Nitric Oxide Production in Patients with Behçet’s Disease

Cited by 62 publications

References 13 publications

Oxidases and oxygenases in regulation of neutrophil redox pathways in Behçet’s disease patients

Oxidases and oxygenases in regulation of neutrophil redox pathways in Behçet’s disease patients

Increased Plasma Adrenomedullin Levels in Patients with Behçet’s Disease

Effect of Plasma from Patients with Behçet’s Disease on the Production of Nitric Oxide in Cultured Human Umbilical Vein Endothelial Cells

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