Decreased oral colonization of Streptococcus mutans during aging of Sprague-Dawley rats

Houte, J. van; Upeslacis, V.N.; Edelstein, Susan

doi:10.1128/iai.16.1.203-212.1977

Cited by 26 publications

(8 citation statements)

References 23 publications

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“…7 of conventional animals. It has been shown that the age of the tooth is critically important for susceptibility to colonization by S. mutans and the resultant formation of caries (22,32). It has been shown that in humans, high counts of salivary lactobacilli can be correlated with prevalance of dental caries (2).…”

Section: Caries Induction In Various Animal Species With a Strain Of mentioning

confidence: 99%

Dental Caries Induction in Experimental Animals by Clinical Strains of Streptococcus mutans Isolated from Japanese Children

Hamada

Ooshima

Torii

et al. 1978

Microbiology and Immunology

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Oral implantation and the cariogenic activity of clinical strains of Streptococcus mutans which had been isolated from Japanese children and labeled with streptomycin-resistance were examined in specific pathogen-free Sprague-Dawley rats. All the seven strains tested were easily implanted and persisted during the experimental period. Extensive carious lesions were produced in rats inoculated with clinical strains of S. mutans belonging to serotypes c, d, e, and f, and maintained on caries-inducing diet #2000. Noninfected rats did not develop dental caries when fed diet #2000. Type d S. mutans preferentially induced smooth surface caries in the rats. Strains of other serotypes primarily developed caries of pit and fissure origin. Caries also developed in rats inoculated with reference S. mutans strains BH-TR and FAlR (type b) that had been maintained in the laboratories for many years. However, the cariogenicity of the laboratory strains was found to have decreased markedly. All three S. sanguis strains could be implanted, but only one strain induced definite fissure caries. Two S. salivarius strains could not be implanted well in the rats and therefore they were not cariogenic. Four different species of lactobacilli also failed to induce dental caries in rats subjected to similar caries test regimen on diet #2000. S. mutans strain MT6R (type c) also induce caries in golden hamsters and ICR mice, but of variable degrees.

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Section: Caries Induction In Various Animal Species With a Strain Of mentioning

confidence: 99%

Dental Caries Induction in Experimental Animals by Clinical Strains of Streptococcus mutans Isolated from Japanese Children

Hamada

Ooshima

Torii

et al. 1978

Microbiology and Immunology

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“…Ninety days after inoculation, all rats were killed by carbon dioxide inhalation and decapitated, and the heads were skinned. Three maxillary and three mandibular molars from two quadrants of the mouth were extracted, ground in tissue grinders, and cultured for enumeration of the test organism on the teeth as described earlier (21).…”

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confidence: 99%

“…At the end of the experiments, 30 days after inoculation, rats were killed for enumeration of the test organisms on the molars of all four quadrants as outlined earlier (21).…”

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confidence: 99%

Role of Colonization in the Virulence of Actinomyces viscosus Strains T14-Vi and T14-Av

Brecher¹,

Houte²,

Hammond

1978

Infect Immun

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Germfree rats fed a high-sucrose diet were inoculated with Actinomyces viscosus strain T14-Vi (virulent) or T14-Av (avirulent). The mean recovery of strain T14-Vi from six extracted finely ground molars of rats sacrificed after 90 days was 1.1 x 108 colony-forming units (CFU). The mean recovery of strain T14-Av was 5.7 x 107 CFU, which was significantly less. Strain T14-Vi caused severe alveolar bone loss, but only minimal bone loss occurred in rats infected with strain T14-Av. Scanning electron microscopy of teeth of germfree rats revealed that strain T14-Vi colonized in the fissures as well as on tooth surface areas near the gingiva; strain T14-Av also colonized in fissures but was unable to colonize the teeth near the gingiva. In studies with conventional rats fed a high-sucrose diet, streptomycin-resistant strain T14-Vi colonized on the teeth of all rats inoculated with in the order of 108 or 107 CFU and on the teeth of about half of the rats inoculated with

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“…Streptomycin-resistant (SR) mutants of S. mutans have been used in many investigations because they can be easily and sensitively quantitated in samples of mixed bacterial populations by use of media containing streptomycin (10,11,19,30,33). Studies which have relied upon use of SR mutants of S. mutans include those investigating: (i) implantation of the organism in the mouths of humans, primates, and rodents (6,8,11,19,21,24,36); (ii) its transmission between individuals (17); (iii) its interdental transmission (7); (iv) its ability to attach to teeth and other oral surfaces (6,31,33,35); (v) its cariogenic potential in animal models (3,10,11,19,24,29); and (vi) the effects of antibodies on its colonization and virulence (8,22,24,25,29). During the course of studying the colonization of gnotobiotic rats by S. mutans strains isolated from humans and rodents, we observed that SR mutants colonized less effectively than wild-type strains; the present report describes these studies.…”

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confidence: 99%

Impaired colonization of gnotobiotic and conventional rats by streptomycin-resistant strains of Streptococcus mutans

Bammann¹,

Clark²,

Gibbons³

1978

Infect Immun

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Colonization of streptomycin-resistant mutants derived from Streptococcus mutans strain LB1, a human isolate, and strain FA-1, a rodent isolate, was studied in gnotobiotic and conventional rats. Mutants resistant to 2.0 mg of streptomycin per ml were isolated by using both stepwise (suffix "R"M) and one-step (suffix "R"1) selections. Rats were infected with mixtures of parental and streptomycinresistant strains, and the proportions of each strain present in samples from the intestinal canal, tongue dorsum, teeth, and fissure plaque were determined. Combinations of strains investigated were LB1 and FA-1"R"M; FA-1 and LB1"R"M; LB1 and LB1"R"1; FA-1 and FA-1"R"1. In gnotobiotic rats, nonresistant strains predominated in every oral sample studied at 7 and 21 days after infection. Similarly, when conventional exgermfree rats were infected with FA-1 and FA-1"R"1, FA-1 dominated in all samples. Streptomycin-sensitive revertants were not detected in rats monoinfected with strains LB1"R"1 and FA-l"R"l for 21 days. No antagonistic interactions were observed between the strains in in vitro experiments. Streptomycin-resistant mutants attached to hydroxyapatite treated with rat or human saliva in equal or higher numbers than did parental strains. However, parental strains appeared to grow faster in Trypticase soy broth than streptomycin-resistant mutants. These observations indicate that induction of streptomycin resistance frequently impairs the colonization properties of S. mutans strains, possibly by altering their rate of growth.

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Decreased oral colonization of Streptococcus mutans during aging of Sprague-Dawley rats

Cited by 26 publications

References 23 publications

Dental Caries Induction in Experimental Animals by Clinical Strains of Streptococcus mutans Isolated from Japanese Children

Dental Caries Induction in Experimental Animals by Clinical Strains of Streptococcus mutans Isolated from Japanese Children

Role of Colonization in the Virulence of Actinomyces viscosus Strains T14-Vi and T14-Av

Impaired colonization of gnotobiotic and conventional rats by streptomycin-resistant strains of Streptococcus mutans

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