Decreased Platelet &lt;sup&gt;3&lt;/sup&gt;H-Paroxetine Binding in Obsessive-Compulsive Patients

Marazziti, Donatella; Rossi, Alessandra; Gemignani, A.; Giannaccini, Gino; Pfanner, Chiara; Milanfranchi, A.; Presta, S.; Lucacchini, Antonio; Cassano, Giovanni B.

doi:10.1159/000119308

Cited by 58 publications

(33 citation statements)

References 17 publications

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“…From a conceptual point of view, the presence of the 5-HT transporter in nucleate cells such as lymphocytes, might permit molecular genetic studies in patients, such as those affected by mood and anxiety disorders, where disturbances at the level of the platelet 5-HT transporter have been reported (Meltzer and Lowy 1987;Marazziti et al 1996), although it is possible that the regulation of the 5-HT transporter may be different in nucleated and nonnucleated cells. Pursuing this line of research, recently Lesch et al (1996) showed that a variant of the polymorphism in the 5-HT transporter reduces the expression of the transporter itself and the 5-HT uptake in lymphoblasts.…”

Section: Discussionmentioning

confidence: 99%

“…Proteins were measured according to the Peterson method (1977) Marazziti et al (1996). Membranes were incubated in a final volume of 2.0 ml B1 buffer.…”

Section: [ 3 H]par Binding Assaymentioning

confidence: 99%

“…However, pharmacological studies in this field have shown a heterogeneity of [ 3 H]IMI binding sites when desipramine is used to define the "specific" binding (Hrdina 1989): desipramine-defined IMI binding appears to consist of two subpopulations, with only that of a proteic nature, 5-HT-sensitive and Na-dependent being present in serotonergic neurons and related to the 5-HT transporter (Marcusson et al 1985). H]PAR), a SSRI, has subsequently seemed to label the 5-HT transporter protein more specifically (Mellerup et al 1983;Marcusson et al 1988), and its use in clinical studies is currently replacing that of [ (D'Haenen et al 1988;Lawrence et al 1993;Faludi et al 1994;Maguire et al 1995;Marazziti et al 1996).…”

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confidence: 99%

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Presence and Characterization of the Serotonin Transporter in Human Resting Lymphocytes

Marazziti

Rossi

Giannaccini

et al. 1998

Neuropsychopharmacology

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Section: Discussionmentioning

confidence: 99%

“…Proteins were measured according to the Peterson method (1977) Marazziti et al (1996). Membranes were incubated in a final volume of 2.0 ml B1 buffer.…”

Section: [ 3 H]par Binding Assaymentioning

confidence: 99%

mentioning

confidence: 99%

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Presence and Characterization of the Serotonin Transporter in Human Resting Lymphocytes

Marazziti

Rossi

Giannaccini

et al. 1998

Neuropsychopharmacology

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“…Par binding was carried out according to the method of Marazziti et al [19]. The incubation mixture consisted of 100 Ìl of platelet membranes (50-100 Ìg protein/tube), 50 Ìl of 3 H-Par at six concentrations ranging from 0.01 to 1 nM, and 1,850 Ìl of assay buffer.…”

Section: Preparation Of Platelet Membranesmentioning

confidence: 99%

Jealousy and Subthreshold Psychopathology: A Serotonergic Link

Marazziti¹,

Rucci²,

Nasso³

et al. 2003

Neuropsychobiology

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Background: A few studies suggest that different neurotransmitters may play a role in the expression of jealousy. Our study aimed to explore the serotonergic system by means of the specific binding of ³H-paroxetine (³H-Par) to platelet membranes of healthy subjects with and without excessive jealousy concerns, according to a specific self-administered questionnaire [Questionnaire for affective relationships (QAR)]. Subjects and Methods: The study sample includes 21 subjects concerned by jealousy thoughts and 21 control subjects without jealousy concerns, as shown by their QAR scores. Subjects of the first group were administered a battery of self-report instruments designed to detect the presence of typical, atypical and subthreshold psychopathology. Platelet membranes and ³H-Par binding were carried out according to standardized protocols. Results: Subjects with excessive jealousy concerns had a reduced density of ³H-Par binding as compared with control subjects without jealousy concerns and had one or more psychiatric spectrum conditions. Conclusions: Our findings suggest that excessive jealousy is associated with various forms of psychopathology and may be underlain by alteration of the serotonergic system, as reflected by the lower density of the platelet serotonin transporter.

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“…Nineteen patients with OCD, out of the total of 21, 20 patients with depression and all healthy controls underwent blood sampling for the evaluation of 3 H-Par binding which was carried out according to the method of Marazziti et al [33]. Briefly, 20 ml of venous blood was drawn from fasting subjects between 8 and 9 a.m., in the months April-June to avoid the possible interference of either circadian or seasonal rhythms, and gently mixed with 1 ml of anticoagulant 0.15 M EDTA.…”

Section: Psychopathological Assessmentmentioning

confidence: 99%

No Correlation between Aggression and Platelet <sup>3</sup>H-Paroxetine Binding in Obsessive-Compulsive Disorder Patients

et al. 2001

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Different findings suggest that the serotonin (5-HT) system may be involved in both the regulation of aggression and the pathophysiology of obsessive-compulsive disorder (OCD). Our study aimed to evaluate the aggressive features of a group of OCD patients and to explore possible correlations with a serotonergic marker, namely platelet 5-HT transporter. Psychopathological and biological patterns were compared with those of a group of healthy controls and those of patients with major depression. Twenty-one patients affected by OCD, 21 by depression and 21 healthy controls were included in the study. Aggressive features were measured by means of the Buss and Durkee Hostility Inventory (BDHI). The platelet 5-HT transporter was evaluated by means of the ³H-paroxetine binding parameters (maximum binding capacity, B_max and dissociation constant, K_d). The OCD patients showed a total score on the BDHI not significantly different from that of healthy controls and lower than that of depressed patients. The factor profile was similar in the 3 groups, but higher in the depressed patients. The irritability, resentment, guilt, negativism and suspiciousness factors were significantly more pronounced in depressed patients. Some sex-related difference in single factors were also observed. The B_max of ³H-paroxetine binding was lower in OCD patients than in depressives or healthy controls. OCD patients were more similar to healthy controls than to depressed patients with regard to aggressive features measured by means of the BDHI. This suggests that aggression in OCD is a complex phenomenon that probably requires specific instruments of evaluation.

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Decreased Platelet <sup>3</sup>H-Paroxetine Binding in Obsessive-Compulsive Patients

Cited by 58 publications

References 17 publications

Presence and Characterization of the Serotonin Transporter in Human Resting Lymphocytes

Presence and Characterization of the Serotonin Transporter in Human Resting Lymphocytes

Jealousy and Subthreshold Psychopathology: A Serotonergic Link

No Correlation between Aggression and Platelet <sup>3</sup>H-Paroxetine Binding in Obsessive-Compulsive Disorder Patients

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