Decreased pool of mesenchymal stem cells is associated with altered chemokines serum levels in atrophic nonunion fractures

Maisonneuve, Mathieu; Rigutto, Sabrina; Ingels, Aude; Spruyt, Delphine; Stricwant, Nadia; Kharroubi, Ilham; Albarani, Valentina; Jayankura, Marc; Rasschaert, Joanne; Bastianelli, Enrico; Gangji, Valérie

doi:10.1016/j.bone.2013.01.005

Cited by 53 publications

(59 citation statements)

References 48 publications

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“…Certain risk factors may predispose a patient to the development of a nonunion, including the type and site of fracture, fracture comminution with bone and soft tissue devascularization, instability, bone loss, presence of a chronic illness, infection and tobacco use. However, while it is known that these risk factors may predispose a patient to develop a nonunion, the underlying physiopathology remains to be fully elucidated (22).…”

Section: Discussionmentioning

confidence: 99%

Adenovirus-mediated bone morphogenetic protein-2 promotes osteogenic differentiation in human mesenchymal stem cells in vitro

Cao

Sun

Zhang

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Delayed and failed bone union following fracture is a common clinical complication that requires treatment in orthopedics. Cell-based therapies and tissue-engineering approaches are potential therapeutic strategies for bone repair and fracture healing. However, the effect of adenovirus expressing bone morphogenetic protein-2 (Ad-BMP-2) on the osteogenic ability of human mesenchymal stem cells (hMSCs) has remained to be fully elucidated. Therefore, in the present study, hMSCs were transduced using Ad-BMP-2 to assess the effects of its application and to determine whether Ad-BMP-2 promotes the osteogenic differentiation of hMSCs. The purity of the hMSC cultures was assessed using flow cytometric analysis. In order to assess the osteogenic activity, alkaline phosphatase activity (ALP) was measured and to estimate the osteoblastic mineralization and calcification, von Kossa staining for phosphates was performed. Cells positive for Src homology 2 domain were determined to be hMSCs and the presence of CD34 was used to distinguish hematopoietic lineages. Following treatment, the Ad-BMP-2 and control group had significantly increased ALP levels (P<0.05). Compared to the blank group and the group transfected with adenoviral vector containing LacZ, the phosphate deposition in the Ad-BMP-2 group and the positive control group treated with dexamethasone was markedly increased. The results of the present study suggested that Ad-BMP-2 promotes osteogenic differentiation in hMSCs and may have a potential application in treating delayed union and nonunion following bone fracture.

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Section: Discussionmentioning

confidence: 99%

Adenovirus-mediated bone morphogenetic protein-2 promotes osteogenic differentiation in human mesenchymal stem cells in vitro

Cao

Sun

Zhang

et al. 2017

Experimental and Therapeutic Medicine

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“…MSCs extracted from non-union tissues have an impaired proliferative capacity and function compared to healthy controls [101][102][103][104]. Nevertheless, these non-union MSCs retain their osteogenic differentiation when activated in vitro [103,105].…”

Section: The Uncontrolled Immune Cell Response and Defective Bone Heamentioning

confidence: 99%

The roles of immune cells in bone healing; what we know, do not know and future perspectives

El-Jawhari

Jones

Giannoudis

2016

Injury

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“…MSCs can be detected within non-union tissues but they have an impaired function including increased their senescence (163). Additionally, it has been demonstrated that the number and the proliferative capacity of BM MSCs can be impaired in patients with non-union fractures compared to healthy controls (164,165). Furthermore, the osteogenic activity is lower compared to BM MSCs (166).…”

Section: Msc-immune Cell Interactions; Implication In Defective Bmentioning

confidence: 99%

“…Furthermore, the osteogenic activity is lower compared to BM MSCs (166). However, these non-union MSCs seem to retain their osteogenic differentiation when activated in vitro (165,167). Additionally, these non-union MSCs can regain a full capacity of osteogenesis if treated with BMP-2 (168).…”

Section: Msc-immune Cell Interactions; Implication In Defective Bmentioning

confidence: 99%

Interactions Between Multipotential Stromal Cells (MSCs) and Immune Cells During Bone Healing

El-Jawhari

Jones

McGonagle

et al. 2016

Recent Advances in Stem Cells

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Abstract:The physiological process of bone healing takes place in three sequential stages: inflammation, repair and remodelling. Multipotential stromal cells (MSCs) are the key progenitor cells for osteoblasts and chondrocytes and are also imbued with immunomodulatory capabilities.Although MSCs are well known to be involved in osteogenesis during the later stages of repair, their role during the inflammatory phase and precise interactions with immune cells remain poorly understood. This chapter describes the current knowledge on cellular interactions during the bone repair as well as cytokines and growth factors mediating these processes. The roles of emerging innate immune cell populations, innate lymphoid cells are also discussed.Based on this current knowledge, we conclude that in addition to their differentiation during later bone repair stages, MSCs are likely to have a substantial involvement in the initial stage of bone healing by controlling the fate of inflammation. An improved understanding of complex cell interactions during bone repair has broad implications on optimising the treatment of the fracture complications including non-union.

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Decreased pool of mesenchymal stem cells is associated with altered chemokines serum levels in atrophic nonunion fractures

Cited by 53 publications

References 48 publications

Adenovirus-mediated bone morphogenetic protein-2 promotes osteogenic differentiation in human mesenchymal stem cells in vitro

Adenovirus-mediated bone morphogenetic protein-2 promotes osteogenic differentiation in human mesenchymal stem cells in vitro

The roles of immune cells in bone healing; what we know, do not know and future perspectives

Interactions Between Multipotential Stromal Cells (MSCs) and Immune Cells During Bone Healing

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