Decreased reactive oxygen species production and NOX1, NOX2, NOX4 expressions contribute to hyporeactivity to phenylephrine in aortas of pregnant SHR

Troiano, Jéssica A.; Potje, Simone R.; Graton, Murilo E.; Cavalari, Priscila; Pereira, Adriana Maria; Vale, Gabriel Tavares do; Nakamune, Ana Cláudia de Melo Stevanato; Sumida, Dóris Hissako; Tirapelli, Carlos Renato; Antoniali, Cristina

doi:10.1016/j.lfs.2015.12.011

Cited by 16 publications

(9 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We observed greater amount of NO in the aorta and mesenteric bed at the end of pregnancy, as indirectly measured by nitrite levels ( Figures 1A , B ). This increase in NO may be associated with increased total eNOS and p-eNOS Ser1177 expression in the aorta and higher NO concentration in aortic endothelial cells from P Wistar rats and P SHR, as already observed by our research team ( Zancheta et al, 2015 ; Troiano et al, 2016 ). Moreover, in the aorta, where NO is the main regulatory factor of vascular homeostasis, variation of NO levels is greater in NP SHR and P SHR than in NP Wistar rats and P Wistar rats, emphasizing the importance of this molecule for regulation of vascular mechanisms during pregnancy of hypertensive rats.…”

Section: Discussionsupporting

confidence: 81%

“…Pregnancy in normotensive Wistar rats and spontaneously hypertensive rats (SHR) is a physiological process characterized by significant reduction in mean arterial pressure despite increased plasma volume. This phenomenon stems from reduced peripheral vascular resistance associated with decreased pressor responses and reduced contractile response to vasoconstrictors ( Barron et al, 1984 ; Chu and Beilin, 1993a , b ; Ballejo et al, 2002 ; Elias et al, 2008 ; Stennett et al, 2009 ; Ognibene et al, 2012 ; Zancheta et al, 2015 ; Troiano et al, 2016 ). Compared to blood vessels from non-pregnant rats, eNOS activity is increased in systemic arteries from late pregnant normotensive and hypertensive rats.…”

Section: Introductionmentioning

confidence: 99%

“…Compared to blood vessels from non-pregnant rats, eNOS activity is increased in systemic arteries from late pregnant normotensive and hypertensive rats. Increased eNOS activity, and hence greater NO production, negatively modulates contraction of systemic blood vessels during pregnancy ( Zancheta et al, 2015 ; Troiano et al, 2016 ). In this study, we hypothesized that pregnancy changes cell signaling involving caveolae, Cav-1, CaM, Hsp90, and eNOS in arteries from normotensive and SHR.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Caveolin-1/Endothelial Nitric Oxide Synthase Interaction Is Reduced in Arteries From Pregnant Spontaneously Hypertensive Rats

et al. 2021

View full text Add to dashboard Cite

We have investigated the role caveolae/caveolin-1 (Cav-1) plays in endothelial nitric oxide synthase (eNOS) activation and how it impacts pregnancy-induced decreased vascular reactivity in normotensive (Wistar rats) and spontaneously hypertensive rats (SHR). Wistar rats and SHR were divided into non-pregnant (NP) and pregnant (P). Nitrite levels were assessed by the Griess method in the aorta and mesenteric vascular bed. In functional studies, arteries were incubated with methyl-β-cyclodextrin (dextrin, 10mmol/L), which disrupts caveolae by depleting cholesterol, and concentration-response curves to phenylephrine (PE) and acetylcholine (ACh) were constructed. Electronic microscopy was used to determine endothelial caveolae density in the aorta and resistance mesenteric artery in the presence of vehicle or dextrin (10mmol/L). Western blot was performed to evaluate Cav-1, p-Cav-1, calmodulin (CaM), and heat shock protein 90 (Hsp90) expression. Cav-1/eNOS interaction in the aorta and mesenteric vascular bed was assessed by co-immunoprecipitation. Nitric oxide (NO) generation was greater in arteries from P groups compared to NP groups. Dextrin did not change vascular responses in the aorta from P groups or the number of caveolae in P groups compared to NP groups. Compared to NP Wistar rats, NP SHR showed smaller number of caveolae and reduced Cav-1 expression. Pregnancy did not alter Cav-1, CaM, or Hsp90 expression in the aorta or mesenteric vascular bed from Wistar rats or SHR. These results suggest that pregnancy does not alter expression of the main eNOS regulatory proteins, but it decreases Cav-1/eNOS interaction. Reduced Cav-1/eNOS interaction in the aorta and mesenteric vascular bed seems to be an important mechanism to increase eNOS activity and nitric oxide production in pregnant normotensive and hypertensive rats.

show abstract

Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Caveolin-1/Endothelial Nitric Oxide Synthase Interaction Is Reduced in Arteries From Pregnant Spontaneously Hypertensive Rats

et al. 2021

View full text Add to dashboard Cite

show abstract

“…10A. In endothelial cells of normotensive and hypertensive male rats [32] and in pregnant and non-pregnant normotensive and hypertensive rats [38] were observed that apocynin stimulated an increase in NO concentration and decreased ROS levels. Moreover, we also observed that apocynin increased Ca +2 concentration ( Fig.…”

Section: Discussionmentioning

confidence: 96%

“…Using a flow cytometer (Attune™ Acoustic Focusing Cytometer, Applied Biosystems, Vic, Australia), samples were excited with a blue laser at 488 nm and emission at 530/30 nm, to DAF-2/DA and FLUO-3AM experiments, and with a blue laser at 488 nm and emission at 585/ 42 nm, to DHE experiments. A software (Attune Cytometric Software, Applied Biosystem) analyzed the cytofluorographic tracings generated and the results were expressed as the mean values of the medians of fluorescence intensity (FI) from the cells stimulated with fluorescent probes in presence or absence of apocynin or diapocynin stimuli, according to Potje et al [37] and Troiano et al [38]. The obtained values were analyzed and compared between cells incubated with apocynin or diapocynin.…”

Section: Flow Cytometry Experiments: Aortic Endothelial Cellsmentioning

confidence: 99%

Hypotensive and vasorelaxant effect of Diapocynin in normotensive rats

Potje

Troiano

Graton

et al. 2017

Free Radical Biology and Medicine

View full text Add to dashboard Cite

Nicotinamide adenine dinucleotide phosphate oxidase (NAD(P)H-oxidase) is a multicomponent enzyme system that generates superoxide anion by one-electron reduction of molecular oxygen and represents the major source of reactive oxygen species (ROS) in the vascular cells. Apocynin has been extensively used as an inhibitor of NADPH oxidase (NOX) in phagocytic cells and as an antioxidant in non-phagocytic cells. In phagocytes cells, due to the presence of myeloperoxidase, apocynin can be the converted to diapocynin, which is supposed to be the active form of this phytochemical. Moreover, apocynin was shown to induce hypotension and vasodilatation in many experimental animal models. However, there are no studies showing the effects of diapocynin on blood pressure or in vascular cells. In this present study, we used chemically synthesized diapocynin and analyzed its antioxidant capacity, effect on blood pressure and vascular reactivity. Moreover, it was evaluated the levels of nitric oxide (NO), ROS and calcium in aortic endothelial cells stimulated by diapocynin. All results were compared to apocynin. We found that diapocynin showed higher antioxidant capacity than apocynin. Apocynin and diapocynin, promoted hypotensive effects without changing the heart rate, however the effects of diapocynin were reversed faster than the effects of apocynin, which was long lasting. Diapocynin and apocynin induced endothelium dependent and independent vasodilatation, but diapocynin was less potent than apocynin regarding the capacity of induction of vasodilatation in mesenteric resistance arteries and aorta from Wistar rats. The relaxation induced by apocynin or diapocynin involves sGC and potassium channels in vascular smooth muscle cells and NOS participates of relaxation induced by apocynin or diapocynin in intact mesenteric rings. Apocynin and diapocynin increased NO and decreased ROS levels in endothelial cells, however diapocynin did not alter calcium levels in these cells. In conclusion, these results demonstrated that, similarly to apocynin, diapocynin also induces hypotensive and vasodilator effects in rats and vascular endothelium improves the diapocynin vasodilator effects by increases NO bioavailability.

show abstract

Bisphenol A: Potential Factor of Miscarriage in Women in the Context of the Phenomenon of Neutrophil Extracellular Traps

Omeljaniuk

Charkiewicz

Garley

et al. 2022

Arch. Immunol. Ther. Exp.

View full text Add to dashboard Cite

Humans are exposed to a number of environmental pollutants every day. Among them, endocrine disruptors are particularly harmful to human health. Bisphenol A (BPA) is a xenoestrogen that has been shown to disrupt the endocrine system and cause reproductive toxicity. In this study, we aimed to verify the potential relationship between BPA and miscarriage involving the formation of neutrophil extracellular traps (NETs). Blood samples were collected from healthy women and women who had miscarriage in the first trimester of pregnancy. The serum levels of cytoplasmic anti-PR3 antibody and perinuclear anti-MPO antibody were determined using an immunoenzymatic method. The concentrations of key proinflammatory proteins TNF-α and MCP-1, as well as NADPH oxidase subunits NOX1 and NCF2, were also measured in the serum samples. The serum concentration of BPA was determined using gas chromatography. The results showed that the concentrations of BPA were significantly elevated in the serum of women who had miscarriage compared to the control group, with the highest concentration found in the “NETs-positive” group. The levels of MCP-1 and TNF-α were significantly higher in the “NETs-positive” group compared to the “NETs-negative” and control group. The levels of NOX1 and NCF2 were also higher in the “NETs-positive” group compared to the “NETs-negative” group. The study showed that BPA could play a role in the course of miscarriage through the formation of NETs. The results indicate the need to limit the exposure of women planning pregnancy to xenoestrogens, including BPA.

show abstract

Decreased reactive oxygen species production and NOX1, NOX2, NOX4 expressions contribute to hyporeactivity to phenylephrine in aortas of pregnant SHR

Abstract: Taken together, these results suggest that ROS production was decreased in the aortas of pregnant SHR and could contribute to higher NO bioavailability and hyporeactivity to PE in the aortas of pregnant SHR.

Cited by 16 publications

References 39 publications

Caveolin-1/Endothelial Nitric Oxide Synthase Interaction Is Reduced in Arteries From Pregnant Spontaneously Hypertensive Rats

Caveolin-1/Endothelial Nitric Oxide Synthase Interaction Is Reduced in Arteries From Pregnant Spontaneously Hypertensive Rats

Hypotensive and vasorelaxant effect of Diapocynin in normotensive rats

Bisphenol A: Potential Factor of Miscarriage in Women in the Context of the Phenomenon of Neutrophil Extracellular Traps

Contact Info

Product

Resources

About