Decreased serum levels of the inflammaging marker miR-146a are associated with clinical non-response to tocilizumab in COVID-19 patients

Sabbatinelli, Jacopo; Giuliani, Angelica; Matacchione, Giulia; Latini, Silvia; Laprovitera, Noemi; Pomponio, Giovanni; Ferrarini, Alessia; Baroni, Silvia; Pavani, Marianna; Moretti, Marco; Gabrielli, Armando; Procopio, Antonio Domenico; Ferracin, Manuela; Bonafè, Massimiliano; Olivieri, Fabiola

doi:10.1016/j.mad.2020.111413

Cited by 100 publications

(116 citation statements)

References 43 publications

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“…Among patients who did not respond to the treatment, the worst test results (the highest increase in plasma IL-6 concentration) occurred in those with the lowest serum miR-146a-5p levels. These data show that miR-146a-5p could serve as a biomarker and provide clues about the molecular relationship between inflammation and the clinical course of COVID-19 [237]. They concluded that blood biomarkers, such as miR-146a-5p, might represent a molecular relationship between inflammation and the clinical course of COVID-19 [237].…”

Section: Sensitivity and Specificity Of Microrna Profiling In Covid-19 Patientsmentioning

confidence: 72%

“…These data show that miR-146a-5p could serve as a biomarker and provide clues about the molecular relationship between inflammation and the clinical course of COVID-19 [237]. They concluded that blood biomarkers, such as miR-146a-5p, might represent a molecular relationship between inflammation and the clinical course of COVID-19 [237]. Sequencing the non-coding RNA and mRNA transcriptomes isolated from red blood cell-depleted whole blood of moderate to severe COVID-19 patients helped define four types of microRNAs with different expression trends that could serve as biomarkers of COVID-19 progression.…”

Section: Sensitivity and Specificity Of Microrna Profiling In Covid-19 Patientsmentioning

confidence: 76%

“…Sabbatinelli et al [237] examined the serum levels of miRNAs regulating inflammation, miR-21-5p, miR-146a-5p, and miR-126-3p in 29 COVID-19 patients receiving tocilizumab with a sex-and age-matched control group. Among patients who did not respond to the treatment, the worst test results (the highest increase in plasma IL-6 concentration) occurred in those with the lowest serum miR-146a-5p levels.…”

Section: Sensitivity and Specificity Of Microrna Profiling In Covid-19 Patientsmentioning

confidence: 99%

See 2 more Smart Citations

Anti-SARS-CoV-2 Strategies and the Potential Role of miRNA in the Assessment of COVID-19 Morbidity, Recurrence, and Therapy

Narożna

Rubiś

2021

IJMS

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Recently, we have experienced a serious pandemic. Despite significant technological advances in molecular technologies, it is very challenging to slow down the infection spread. It appeared that due to globalization, SARS-CoV-2 spread easily and adapted to new environments or geographical or weather zones. Additionally, new variants are emerging that show different infection potential and clinical outcomes. On the other hand, we have some experience with other pandemics and some solutions in virus elimination that could be adapted. This is of high importance since, as the latest reports demonstrate, vaccine technology might not follow the new, mutated virus outbreaks. Thus, identification of novel strategies and markers or diagnostic methods is highly necessary. For this reason, we present some of the latest views on SARS-CoV-2/COVID-19 therapeutic strategies and raise a solution based on miRNA. We believe that in the face of the rapidly increasing global situation and based on analogical studies of other viruses, the possibility of using the biological potential of miRNA technology is very promising. It could be used as a promising diagnostic and prognostic factor, as well as a therapeutic target and tool.

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Section: Sensitivity and Specificity Of Microrna Profiling In Covid-19 Patientsmentioning

confidence: 72%

Section: Sensitivity and Specificity Of Microrna Profiling In Covid-19 Patientsmentioning

confidence: 76%

Section: Sensitivity and Specificity Of Microrna Profiling In Covid-19 Patientsmentioning

confidence: 99%

See 1 more Smart Citation

Anti-SARS-CoV-2 Strategies and the Potential Role of miRNA in the Assessment of COVID-19 Morbidity, Recurrence, and Therapy

Narożna

Rubiś

2021

IJMS

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show abstract

“…111 miR-146a-5p transcription is also regulated by NF-κB, 112 and patients with COVID-19 have been shown to have higher levels of IL-6 and lower levels of miR-146a-5p than average, suggesting imbalances in the physiological axis of IL-6/miR-146a-5p in the pathogenesis of COVID-19 infections. 113 TQ treatment, however, controls miR146a expression and can therefore reduce inflammatory reactions by interfering with NF-kB. 114…”

Section: Epigenetic Modulatory Effectmentioning

confidence: 99%

Thymoquinone: A Promising Natural Compound with Potential Benefits for COVID-19 Prevention and Cure

Badary¹,

Hamza²,

Tikamdas³

2021

DDDT

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“…Several studies explored the interaction between miRNAs and SARS-CoV-2, which suggested potential therapeutic outcomes [ 19 ]. However, in COVID-19 patients with multifocal interstitial pneumonia, low serum levels of miRNAs related to the regulation of inflammation were associated with poor prognosis, high mortality rate, and subtherapeutic outcome [ 20 ]. On another note, miRNAs were explored in COVID-19 several organ complications.…”

Section: Introductionmentioning

confidence: 99%

Circulating Soluble ACE2 and Upstream microRNA Expressions in Serum of Type 2 Diabetes Mellitus Patients

Elemam

Hasswan

Aljaibeji

et al. 2021

IJMS

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The global coronavirus disease 2019 (COVID-19) pandemic was associated with multiple organ failure and comorbidities, such as type 2 diabetes mellitus (T2DM). Risk factors, such as age, gender, and obesity, were associated with COVID-19 infection. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is known to use several host receptors for viral entry, such as angiotensin-converting enzyme 2 (ACE2) and transmembrane protease serine 2 (TMPRSS2) in the lung and other organs. However, ACE2 could be shed from the surface to be soluble ACE2 (sACE2) in the circulation. The epigenetic factors affecting ACE2 expression include a type of small non-coding RNAs called microRNAs (miRNAs). In this study, we aimed at exploring the status of the sACE2 as well as serum levels of several upstream novel miRNAs as non-invasive biomarkers that might have a potential role in T2DM patients. Serum samples were collected from 50 T2DM patients and 50 healthy controls, and sACE2 levels were quantified using enzyme-linked immunosorbent assay (ELISA). Also, RNA was extracted, and TaqMan miRNA reverse transcription quantitative PCR (RT-qPCR) was performed to measure serum miRNA levels. Our results revealed that sACE2 is decreased in the T2DM patients and is affected by age, gender, and obesity level. Additionally, 4 miRNAs, which are revealed by in silico analysis to be potentially upstream of ACE2 were detectable in the serum. Among them, miR-421 level was found to be decreased in the serum of diabetic patients, regardless of the presence or absence of diabetic complications, as well as being differential in various body mass index (BMI) groups. The other 3 miRNAs (miR-3909, miR-212-5p, and miR-4677-3p) showed associations with multiple factors including age, gender, BMI, and serum markers, in addition to being correlated to each other. In conclusion, our study reveals a decline in the circulating serum levels of sACE2 in T2DM patients and identified 4 novel miRNAs that were associated with T2DM, which are influenced by different clinical and demographic factors.

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Decreased serum levels of the inflammaging marker miR-146a are associated with clinical non-response to tocilizumab in COVID-19 patients

Cited by 100 publications

References 43 publications

Anti-SARS-CoV-2 Strategies and the Potential Role of miRNA in the Assessment of COVID-19 Morbidity, Recurrence, and Therapy

Anti-SARS-CoV-2 Strategies and the Potential Role of miRNA in the Assessment of COVID-19 Morbidity, Recurrence, and Therapy

Thymoquinone: A Promising Natural Compound with Potential Benefits for COVID-19 Prevention and Cure

Circulating Soluble ACE2 and Upstream microRNA Expressions in Serum of Type 2 Diabetes Mellitus Patients

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