Decursin fromAngelica gigasNakai Inhibits B16F10 Melanoma Growth Through Induction of Apoptosis

Abstract

Decursin, a bioactive phytochemical isolated from Angelica gigas Nakai (danggwi), has shown preclinical anticancer efficacy in various cancer models. However, the antitumor effect of decursin in melanoma models remains undefined. The antitumor activities of decursin were investigated in B16F10 cells in vitro and in vivo. In this study, we show that treatment with decursin inhibited cell proliferation in a dose-dependent manner in B16F10 cells, but not in normal cells. Decursin also induced apoptosis in B16F10 cells, as determined by annexin V-staining assay and transferase-mediated nick-end labeling (TUNEL) staining assay. Decursin increased the phosphorylation of p38 as well as the expression of Bax while decreasing the phosphorylation of extracellular signaling-regulated kinase (ERK) and the expression of Bcl-2 in B16F10 cells. Moreover, decursin activated caspase-3 in B16F10 cells and xenograft tumor tissue. Together, these findings support further investigations into the potential use of decursin in the treatment of melanoma cells.

SH003-induced G1 phase cell cycle arrest induces apoptosis in HeLa cervical cancer cells

“…SH003 comprises Am, Ag and Tk, which have previously been indicated as effective treatments for a variety of diseases, such as hematologic diseases, endocrine disorders, leukemia, hepatocellular carcinoma, colon cancer, non-small cell lung cancer and gastric cancer (14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25). Although the mechanism of action remains unclear, the mixture of Ag, Am and Tk may complement the individual properties of each component.…”

“…Our previous study developed a new herbal medicine, called SH003, which is composed of Astragalus membranaceus (Am), Angelica gigas (Ag) and Trichosanthes kirilowii Maximowicz (Tk), based on the principles of traditional Chinese medicine (12,13). Am and Ag have been reported to be effective treatments for a number of diseases, including hematologic diseases, endocrine disorders and cancers (14)(15)(16)(17)(18)(19). Therapeutic effects of Tk have also been indicated for various cancer types, such as leukemia, hepatocellular carcinoma, colon cancer, non-small cell lung cancer and gastric cancer (18,(20)(21)(22)(23)(24)(25).…”

“…Therapeutic effects of Tk have also been indicated for various cancer types, such as leukemia, hepatocellular carcinoma, colon cancer, non-small cell lung cancer and gastric cancer (18,(20)(21)(22)(23)(24)(25). Several previous studies have demonstrated that a mixture of those components named SH003 was better therapeutic effect than each component in other types of cancer (12,13,17,(26)(27)(28)(29)(30). Thus, SH003 is likely to be used for cancer treatment as novel herbal mixture.…”

Silencing of astrocyte elevated gene‐1 inhibits proliferation and migration of melanoma cells and induces apoptosis

“…Melanoma is a skin malignancy originating from melanocytes; it has a high mortality rate and a poor 5‐year survival rate . Although it accounts for approximately 4% of skin cancers, melanoma accounts for nearly 75% of skin cancer‐related deaths …”

Tanapoxvirus lacking a neuregulin-like gene regresses human melanoma tumors in nude mice

“…It accounts for approximately 4% of all skin cancers, but for nearly 80% of all skin-cancer related deaths [1–3]. If not detected at the early stage, metastatic melanoma is highly aggressive with the five-year survival rate of approximately 15% [4].…”