Deep eutectic solvents for biocatalytic transformations: focused lipase-catalyzed organic reactions

Tan, Jia‐Neng; Yuqing, Dou

doi:10.1007/s00253-019-10342-y

Cited by 71 publications

(33 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lipases are one of the most used enzymes in industry [9,79,81,[162][163][164]. They belong to the class of hydrolases (EC 3.1.1.1) and their natural function is the hydrolysis of triglycerides to fatty acids and glycerol.…”

Section: Lipases As Industrial Biocatalystsmentioning

confidence: 99%

One Pot Use of Combilipases for Full Modification of Oils and Fats: Multifunctional and Heterogeneous Substrates

et al. 2020

View full text Add to dashboard Cite

Lipases are among the most utilized enzymes in biocatalysis. In many instances, the main reason for their use is their high specificity or selectivity. However, when full modification of a multifunctional and heterogeneous substrate is pursued, enzyme selectivity and specificity become a problem. This is the case of hydrolysis of oils and fats to produce free fatty acids or their alcoholysis to produce biodiesel, which can be considered cascade reactions. In these cases, to the original heterogeneity of the substrate, the presence of intermediate products, such as diglycerides or monoglycerides, can be an additional drawback. Using these heterogeneous substrates, enzyme specificity can promote that some substrates (initial substrates or intermediate products) may not be recognized as such (in the worst case scenario they may be acting as inhibitors) by the enzyme, causing yields and reaction rates to drop. To solve this situation, a mixture of lipases with different specificity, selectivity and differently affected by the reaction conditions can offer much better results than the use of a single lipase exhibiting a very high initial activity or even the best global reaction course. This mixture of lipases from different sources has been called “combilipases” and is becoming increasingly popular. They include the use of liquid lipase formulations or immobilized lipases. In some instances, the lipases have been coimmobilized. Some discussion is offered regarding the problems that this coimmobilization may give rise to, and some strategies to solve some of these problems are proposed. The use of combilipases in the future may be extended to other processes and enzymes.

show abstract

Section: Lipases As Industrial Biocatalystsmentioning

confidence: 99%

One Pot Use of Combilipases for Full Modification of Oils and Fats: Multifunctional and Heterogeneous Substrates

et al. 2020

View full text Add to dashboard Cite

show abstract

“…To this point, hydrolases are the most studied enzymes in DESs, 12, 19 which is not surprising given that lipases are the most important industrial enzymes. Meanwhile, there are a limited number of papers dealing with synthetic reactions in DES involving other hydrolytic enzymes (epoxide hydrolases, phospholipases, proteases, and haloalkane dehalogenases), lyases, and dehydrogenases (usually as a part of a whole‐cell biocatalysis) 20, 21 . The most significant achievements in the area are summarized in Tables 2 and 3.…”

Section: The Role Of Dess In Biocatalysismentioning

confidence: 99%

Designing a biocatalytic process involving deep eutectic solvents

Panić

Bubalo

Redovniković

2020

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

During the last decade, deep eutectic solvents (DESs) have emerged as a promising alternative to traditional organic solvents, from both environmental and technological perspectives. The number of structural combinations encompassed by DESs is tremendous; thus, it is possible to design an optimal DES for each specific enzymatic reaction system. In (bio)catalytic processes, a DES can serve as solvent/co-solvent, as an extractive reagent for an enzymatic product, and as a pretreatment solvent of enzymatic biomass. To date, hydrolases are the most studied enzymes in DESs, which is not surprising given that lipases are the most important industrial enzymes. At the same time, there are a limited number of papers dealing with synthetic reactions in DESs involving other hydrolytic enzymes (epoxide hydrolases, phospholipase, proteases and haloalkane dehalogenases), lyases, and dehydrogenases (as a part of the whole Saccharomyces cerevisae and Escherichia coli cell biocatalysis). When designing efficient biocatalytic processes involving DESs, independent of the reaction type and enzyme used, the following steps should be included: (i) preparation and characterisation of the DES, (ii) screening of the DES for optimal enzyme performance, (iii) selection and optimisation of the biocatalytic protocol, and (iv) recovery of the product/DES and DES recycling with possible scale-up. In this paper, we will present some practical aspects that we experienced while working with these solvents, together with some major observations that are available in the literature.

show abstract

“…In some cases, the stability of enzymes can even be enhanced. 65 Nevertheless, in many cases the product recovery is still from water (since enzymes are most usually used in an aqueous environment). This further complicates separation because evaporation costs become significant at scale.…”

Section: Required Conditions For Industrial Operationmentioning

confidence: 99%

Advances in biological conversion technologies: new opportunities for reaction engineering

Woodley

2020

React. Chem. Eng.

View full text Add to dashboard Cite

show abstract

Deep eutectic solvents for biocatalytic transformations: focused lipase-catalyzed organic reactions

Cited by 71 publications

References 84 publications

One Pot Use of Combilipases for Full Modification of Oils and Fats: Multifunctional and Heterogeneous Substrates

One Pot Use of Combilipases for Full Modification of Oils and Fats: Multifunctional and Heterogeneous Substrates

Designing a biocatalytic process involving deep eutectic solvents

Advances in biological conversion technologies: new opportunities for reaction engineering

Contact Info

Product

Resources

About