For float glass, there is a correlation between the striae in end image and the manufacturing process. If clearly understood, the correlation helps to optimize and fine-tune the manufacturing process of float glass. This paper attempts to extract the striae from the end image of float glass with deep learning (DL) neural network (NN). For this purpose, an image segmentation model was established based on improved U-Net, a fully convolutional network (FCN), and used to accurately divide the glass liquid on the end image into different layers. Firstly, the improved U-Net model was constructed to extract the striae from each liquid layer on the end image. Next, the activation function and convolutional mode of the improved U-Net model were optimized to enhance the segmentation accuracy and shorten the training/prediction time. Finally, the proposed model was tested on the float glass production line of Hebei CSG Glass Co., Ltd. The test results show that our model achieved an accuracy of 94%. The research findings lay a solid basis for striae identification on end image of float glass, and provide guidance for optimization and fine-tuning of float glass manufacturing process.