Deep-sea mercury resistant bacteria from the Central Indian Ocean: A potential candidate for mercury bioremediation

Joshi, Gajendra; Meena, Balakrishnan; Verma, Pankaj; Nayak, Jibananand; Vinithkumar, Nambali Valsalan; Dharani, G.

doi:10.1016/j.marpolbul.2021.112549

Cited by 15 publications

(15 citation statements)

References 47 publications

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“…To isolate and enumerate total culturable bacteria (TCB) and culturable mercury-resistant bacteria (CMRB) under aerobic conditions, the previously described serial dilution method was adopted (Joshi et al, 2021). In brief, the stock solution (1 g/L) of mercuric chloride (HgCl 2 , Merck) was prepared and the stability of the Hg stock solution was ensured by reducing the pH level below 2.0 using Suprapur elemental grade nitric acid (HNO 3 , Merck, India).…”

Section: Isolation and Screening Of Total Culturable Bacteria (Tcb) A...mentioning

confidence: 99%

“…A conserved region of merA gene encoding bacterial mercuric reductase enzyme was detected using the above isolated genomic DNA (Joshi et al, 2021). The PCR reactions were carried out using the primer set F1merA (5'-TCGTGATGTTCGACCGCT-3') and F2merA (5'-TACTCCCGCCGTTTCCAAT-3') containing 1 U/ml Taq polymerase (Sigma-Aldrich), 1 × Enzyme buffer, 200 mM of each dNTP (Sigma-Aldrich), 1.25 mM MgCl 2 , and 0.5 mM of each primer and 50 ng template DNA in a thermal cycler (Applied Biosystems).…”

Section: Genotyping Of Mera Genementioning

confidence: 99%

“…A simplified X-ray film method was adopted to look into the Hg reduction by bacterial volatilization in the presence of 50 and 100 mg/ L of Hg as HgCl 2 (Nakamura and Nakahara, 1988;Joshi et al, 2021).…”

Section: Confirmation Of Hg 2+ To Hg 0 Reduction By the Isolatesmentioning

confidence: 99%

“…The cultures were grown in a ZMB medium supplemented with 50 mg/L of HgCl 2 at 30°C and 150 rpm for 48 h. To collect the Hg associated with the bacterial cells, the cultures were centrifuged at 12,000 rpm for 10 min. To analyse the Hg concentration, the samples were processed as described by (Joshi et al 2021). The detailed procedure for Hg estimation is elaborated in the SI.…”

Section: Determination Of Hg(ii) Depletion Potential By Isolatesmentioning

confidence: 99%

“…In our recent study (Joshi et al, 2021), we isolated and characterized several MRBs from the deeper depth of the Central Indian Ocean and evaluated their ability to remove Hg from the culture media. However, no bacterial strain was observed from the surface seawater samples that were resistant to Hg up to 100 mg/L.…”

Section: Introductionmentioning

confidence: 99%

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Unraveling the potential of bacteria isolated from the equatorial region of Indian Ocean in mercury detoxification

et al. 2022

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The marine environment is most vital and flexible with continual variations in salinity, temperature, and pressure. As a result, bacteria living in such an environment maintain the adaption mechanisms that are inherent in unstable environmental conditions. The harboring of metal-resistant genes in marine bacteria contributes to their effectiveness in metal remediation relative to their terrestrial counterparts. A total of four mercury-resistant bacteria (MRB) i.e. NIOT-EQR_J7 (Alcanivorax xenomutans); NIOT-EQR_J248 and NIOT-EQR_J251 (Halomonas sp.); and NIOT-EQR_J258 (Marinobacter hydrocarbonoclasticus) were isolated from the equatorial region of the Indian Ocean (ERIO) and identified by analyzing the 16S rDNA sequence. The MRBs can reduce up to 70% of Hg(II). The mercuric reductase (merA) gene was amplified and the mercury (Hg) volatilization was confirmed by the X-ray film method. The outcomes obtained from ICP-MS validated that the Halomonas sp. NIOT-EQR_J251 was more proficient in removing the Hg from culture media than other isolates. Fourier transform infrared (FT-IR) spectroscopy results revealed alteration in several functional groups attributing to the Hg tolerance and reduction. The Gas Chromatography-Mass Spectrometry (GC-MS) analysis confirmed that strain Halomonas sp. (NIOT-EQR_J248 and NIOT-EQR_J251) released Isooctyl thioglycolate (IOTG) compound under mercury stress. The molecular docking results suggested that IOTG can efficiently bind with the glutathione S-transferase (GST) enzyme. A pathway has been hypothesized based on the GC-MS metabolic profile and molecular docking results, suggesting that the compound IOTG may mediate mercuric reduction via merA-GST related detoxification pathway.

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Section: Isolation and Screening Of Total Culturable Bacteria (Tcb) A...mentioning

confidence: 99%

Section: Genotyping Of Mera Genementioning

confidence: 99%

Section: Confirmation Of Hg 2+ To Hg 0 Reduction By the Isolatesmentioning

confidence: 99%

Section: Determination Of Hg(ii) Depletion Potential By Isolatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Unraveling the potential of bacteria isolated from the equatorial region of Indian Ocean in mercury detoxification

et al. 2022

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Study of mercury resistance and Fourier transform infrared spectroscopy‐based metabolic profiling of a potent Bacillus tropicus strain from forest soil

Saha,

Roy,

Saha

et al. 2023

J Basic Microbiol

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Mercury (Hg) is a highly toxic heavy metal and Hg‐resistant indigenous bacterial isolates may offer a green and cost‐effective bioremediation strategy to counter Hg contamination. In this study, a potent Hg‐resistant bacterium was isolated from the forest soil of a bird sanctuary. Identification using matrix‐assisted laser desorption ionization‐time of flight mass spectrometry depicted the isolate as a strain of Bacillus tropicus, validated by morphological, biochemical, and molecular studies. The isolate demonstrated biological Hg removal efficiency and capacity of 50.67% and 19.76 mg g−1, respectively. The plasmid borne resistance determinant, merA, encoding mercuric reductase, was detected in the bacterium endowing it with effective Hg volatilization and resistance capability. A Fourier‐transform infrared spectroscopic comparative metabolic profiling revealed the involvement of various functional groups like –COOH, ‐CH2, ‐OH, PO4− and so on, resulting in differential spectral patterns of the bacterium both in control and Hg‐exposed situations. A temporal variance in metabolic signature was also observed during the early and mid‐log phase of growth in the presence of Hg. The bacterium described in this study is the first indigenous Hg‐resistant strain isolated from the Uttar Dinajpur region, which could be further explored and exploited as a potent bioresource for Hg remediation.

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Bioremediation of mercury contaminated soil and water: A review

Kumar,

Chawla

et al. 2023

Land Degrad Dev

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Mercury (Hg) pollution of soil and water environments is a major global threat to human health, agri‐food systems and ecosystems and industrial activities mainly coal combustion augmented their content in different environmental media. Bioremediation is a nature‐based solution involving microbial‐ and plant‐based (phytoremediation) technologies that clean‐up Hg contaminated sites. Here, we review Hg‐resistant bacteria and how latest insights in our understanding of the cellular and biochemical mechanisms of the mer operon genes responsible for Hg resistance and transformation have facilitated developments in microbial Hg‐bioremediation. We also review the phytoremediation mechanisms, including those of bacterial‐ and fungi‐assisted phytoremediation processes, which have shown promising results in reducing Hg2+ to Hg0. This review provides a detailed knowledge of novel Hg bioremediation mechanisms and methods. Consequently, microbial‐ and phyto‐based bioremediation technologies have a critical role in the reclamation of Hg‐contaminated environments and the protection of human health and ecosystems.

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Deep-sea mercury resistant bacteria from the Central Indian Ocean: A potential candidate for mercury bioremediation

Cited by 15 publications

References 47 publications

Unraveling the potential of bacteria isolated from the equatorial region of Indian Ocean in mercury detoxification

Unraveling the potential of bacteria isolated from the equatorial region of Indian Ocean in mercury detoxification

Study of mercury resistance and Fourier transform infrared spectroscopy‐based metabolic profiling of a potent Bacillus tropicus strain from forest soil

Bioremediation of mercury contaminated soil and water: A review

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