2016
DOI: 10.3390/ijms17020200
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Deep Sequencing and Screening of Differentially Expressed MicroRNAs Related to Milk Fat Metabolism in Bovine Primary Mammary Epithelial Cells

Abstract: Milk fat is a key factor affecting milk quality and is also a major trait targeted in dairy cow breeding. To determine how the synthesis and the metabolism of lipids in bovine milk is regulated at the miRNA level, primary mammary epithelial cells (pMEC) derived from two Chinese Holstein dairy cows that produced extreme differences in milk fat percentage were cultured by the method of tissue nubbles culture. Small RNA libraries were constructed from each of the two pMEC groups, and Solexa sequencing and bioinfo… Show more

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Cited by 41 publications
(48 citation statements)
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“…These highly, commonly expressed miRNAs suggest their potential regulatory functions in mammary gland development and productivity. This notion is supported by biological process and pathway enrichment results since many basic cellular and molecular processes2728 and immunlogical pathways were enriched for these commonly highly expressed miRNAs (Table 2). …”
Section: Discussionmentioning
confidence: 68%
“…These highly, commonly expressed miRNAs suggest their potential regulatory functions in mammary gland development and productivity. This notion is supported by biological process and pathway enrichment results since many basic cellular and molecular processes2728 and immunlogical pathways were enriched for these commonly highly expressed miRNAs (Table 2). …”
Section: Discussionmentioning
confidence: 68%
“…A comparison of milk microRNA contents with mammary gland cell profiles by small RNA sequencing (Hou et al., ; Ju et al., ; Li et al., ; Luoreng, Wang, Mei, & Zan, ; Shen et al., ) supports the epithelial origin of these microRNAs because the most enriched microRNAs in milk are also highly enriched in mammary glands cells and are of high importance for lactation (Chen et al., ; Do et al., , ; Gu et al., ; Muroya et al., ).…”
Section: Cellular Origin Of Milk Micrornasmentioning
confidence: 98%
“…The majority of studies have only investigated this matter in silico using microRNA‐target identification tools and associated pathways/gene ontology analysis (Alsaweed et al., , ; Cai et al., , ; Carney et al., ; Chen et al., , , ; Chokeshaiusaha, Sananmuang, Puthier, & Nguyen, ; Do, Li, Dudemaine, & Ibeagha‐Awemu, ; Golan‐Gerstl, Elbaum Shiff, et al., ; Gu et al., , ; Hou et al., ; Kang et al., ; Li et al., , ; Liao et al., ; Liu et al., ; Lukasik & Zielenkiewicz, ; Modepalli et al., ; Munch et al., ; Shen et al., ; Simpson et al., ; Sun et al., ; Wang et al., ; Zhang et al., ; Zhou et al., ).…”
Section: Milk Micrornas In Health and Diseasementioning
confidence: 99%
“…According to our previous study, bta‐miR‐152 is down‐regulated in low milk fat breast tissue, and the target genes show up‐regulation trend are PRKAA1 (protein kinase, AMP‐activated, alpha 1 catalytic subunit) and UCP3 (uncoupling proteins 3) (Shen et al, ). PRKAA1 is an AMP‐induced protein kinase that plays an important regulatory role in energy metabolism, particularly in the metabolism of sugars and fats.…”
Section: Introductionmentioning
confidence: 93%
“…According to our previous study, bta-miR-152 is down-regulated in low milk fat breast tissue, and the target genes show up-regulation trend are PRKAA1(protein kinase, AMP-activated, alpha 1 catalytic subunit) and UCP3 (uncoupling proteins 3) (Shen et al, 2016).…”
mentioning
confidence: 90%