Deep sequencing detects human papillomavirus (HPV) in cervical cancers negative for HPV by PCR

Mühr, Laila Sara Arroyo; Lagheden, Camilla; Lei, Jiayao; Eklund, Carina; Kleppe, Sara Nordqvist; Sparén, Pär; Sundström, Karin; Dillner, Joakim

doi:10.1038/s41416-020-01111-0

Cited by 41 publications

(51 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is in agreement with a meta‐analysis by Li et al, who reported a proportion of 0.5% of HPV73 among 12 588 invasive cancers 27 . However, the prevalence of HPV73 in cancers appears to vary between regions and HPV73 was ranked as the fourth most common type in the Oceania region based on data published from 1990 to 2010, 27 and was the ninth most common type among cervical cancer in Sweden during 2002 to 2011 26 . Additionally, Wallin et al demonstrated that the population‐based HPV 73 seroprevalence in Sweden was 14% 28 …”

Section: Discussionsupporting

confidence: 90%

“…At present, HPV73 is classified as possibly carcinogenic 2 . A mean HPV73‐prevalence of 0.62% (76/16027, range 0.35%‐15%) was observed by compiled results from six studies of invasive cervical cancer 2,10,23‐26 . This is in agreement with a meta‐analysis by Li et al, who reported a proportion of 0.5% of HPV73 among 12 588 invasive cancers 27 .…”

Section: Discussionsupporting

confidence: 88%

“…Based on our Aptima HPV results of invasive cancer from 2016 to 2019, the proportion of HPV positive cancers would have been increased by at least 2.5% units (from 93.6% [193/206] to 96.1% [198/206]) if HPV73 had been detectable by the Aptima HPV assay. Furthermore, Arroyo Mühr et al recently reported that HPV73 was found in 5.8% (23/392) of HPV‐MGP‐PCR negative cervical cancers by the use of deep sequencing 26 . Overall, inclusion of HPV73 detection in commercial screening platforms will most likely reduce cervical cancer cases in a primary HPV screening setting, without significantly increased cytology evaluations since HPV73 is a relatively rare HPV type (mean 0.3% of 116 913 women) among women with normal cytology 29 .…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

HPV73 in cervical cancer and distribution of HPV73 variants in cervical dysplasia

Borgfeldt

Söderlund-Strand

Flygh

et al. 2021

Intl Journal of Cancer

View full text Add to dashboard Cite

HPV73 is classified as possibly oncogenic and is not recognized by most commercial primary HPV screening platforms. The aim was to determine the prevalence of HPV73 among invasive cervical cancers, formalin‐fixed paraffin embedded (FFPE) samples (N = 69), from southern Sweden during 2009 to 2010. Another aim was to determine proportions of HPV73 among Aptima HPV assay negative cervical cancers (N = 9, out of 206 cancers) and of high‐grade cytological cervical diagnosis (N = 75, out of 5807 high grade lesions) in liquid‐based cytology (LBC) samples collected between 2016 and 2019. We also investigated the distribution of HPV73 variants A1, A2 and B among HPV73‐positive cases. HPV73 was detected by multiplex MGP‐PCR and Luminex, and HPV73 variants were identified by sequencing PCR amplicons. HPV73 was detected in 2.9% (2/69, 95% CI: 0.18‐9.9) of the FFPE cervical cancer series. Among the Aptima HPV‐negative LBC samples, HPV73 was present in 55.5% (5/9) of the cancers and 29.3% (22/75) of the different grades of cervical diagnosis. The A1, A2 and B variants were present in 6.9% (2/29), 82.7% (24/29) and 10.3% (3/29) of the HPV73‐positive women, respectively. Among the seven HPV73 cancer cases (two FFPE samples and five LBC samples), six A2 and one A1 isolate were detected. In summary, the A2 variant of HPV73 was most common in our region. In addition, the observed prevalence of HPV73 (2.9%) in cervical cancers and its relative high occurrence (55.5%) among Aptima HPV‐negative cancers urge that detection of HPV73 should be included in future primary HPV screening programs.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

HPV73 in cervical cancer and distribution of HPV73 variants in cervical dysplasia

Borgfeldt

Söderlund-Strand

Flygh

et al. 2021

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…The remaining "other" histological types of cervical cancers that we did not identify in this study typically constitute <4% of cervical cancers. 24 Two hundred cases did not have a cervical sample with an HPV analysis prior to diagnosis and were excluded. Altogether 319 cases with 1424 samples were identified.…”

Section: Study Populationmentioning

confidence: 99%

Human papillomavirus load and genotype analysis improves the prediction of invasive cervical cancer

Hortlund

Mol

Pol

et al. 2021

Intl Journal of Cancer

Self Cite

View full text Add to dashboard Cite

Human papillomavirus (HPV)-based cervical screening is a globally recommended health policy. Different HPV types have different risk for cervical cancer. For optimal HPV screening, the sensitivity and specificity for each HPV type at different viral loads should be known in a screening setting. HPV test results in about 1 million cervical samples analyzed during 2006 to 2014 were compared for 319 women who had developed invasive cervical cancer up to 8.5 years later and for 1911 matched control women. Detection including low viral loads resulted in markedly increased sensitivity for cervical cancer only for HPV types 16 and 18. Testing for HPV types 31, 33, 45 and 52 also increased the sensitivity for prediction of cervical cancer, but for these viruses, detection of low viral load did not further increase sensitivity. HPV types 35, 39, 51, 56, 58, 59, 66 and 68 only predicted occasional additional cervical cancer cases.Testing for HPV16/18 at low viral load plus testing for HPV31, 33, 45 and 52 at >3000 copies/μL predicted 86.5% of cancers occurring within a year after testing, similar to the 89.4% that were predicted by testing for 14 HPV types. By contrast, the type and viral load-restricted testing greatly increased specificity: 6.3% of healthy women tested positive as compared to 11.7% of healthy women testing positive for the 14 HPV types commonly screened for today. Adequate HPV screening sensitivity, with considerable increase in specificity, can be obtained by testing only for HPV16/18/31/33/45/52, with detection of low viral load required only for HPV16/18.

show abstract

“…Based on the official PV nomenclature, PV’s taxonomic ranking is set according to the similarity of the highly conserved full-length L1 genes at the 60% nucleotide sequence similarity for genera, 70% for species and 90% for types [ 6 , 7 ]. With 224 human (HPV) and 215 animal PV types currently characterized and officially recognized, Papillomaviridae is one of the largest viral families infecting vertebrates [ 8 , 9 ]. In addition, complete PV genome sequences annotated with clinical and biochemical data that are readily available make PVs an attractive research model for study of the evolution of pathogen-host interactions [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

First Report of Phodopus sungorus Papillomavirus Type 1 Infection in Roborovski Hamsters (Phodopus roborovskii)

Domjanič

Hošnjak

Lunar

et al. 2021

Viruses

View full text Add to dashboard Cite

Papillomaviruses (PVs) are considered highly species-specific with cospeciation as the main driving force in their evolution. However, a recent increase in the available PV genome sequences has revealed inconsistencies in virus–host phylogenies, which could be explained by adaptive radiation, recombination, host-switching events and a broad PV host range. Unfortunately, with a relatively low number of animal PVs characterized, understanding these incongruities remains elusive. To improve knowledge of biology and the spread of animal PV, we collected 60 swabs of the anogenital and head and neck regions from a healthy colony of 30 Roborovski hamsters (Phodopus roborovskii) and detected PVs in 44/60 (73.3%) hamster samples. This is the first report of PV infection in Roborovski hamsters. Moreover, Phodopus sungorus papillomavirus type 1 (PsuPV1), previously characterized in Siberian hamsters (Phodopus sungorus), was the only PV detected in Roborovski hamsters. In addition, after a detailed literature search, review and summary of published evidence and construction of a tanglegram linking the cladograms of PVs and their hosts, our findings were discussed in the context of available knowledge on PVs described in at least two different host species.

show abstract

Deep sequencing detects human papillomavirus (HPV) in cervical cancers negative for HPV by PCR

Cited by 41 publications

References 26 publications

HPV73 in cervical cancer and distribution of HPV73 variants in cervical dysplasia

HPV73 in cervical cancer and distribution of HPV73 variants in cervical dysplasia

Human papillomavirus load and genotype analysis improves the prediction of invasive cervical cancer

First Report of Phodopus sungorus Papillomavirus Type 1 Infection in Roborovski Hamsters (Phodopus roborovskii)

Contact Info

Product

Resources

About